| 质粒名称: | pAL149 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22275 |
| 存储实验室: | Christopher Voigt |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，合成生物学 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pCMV-PhyB(1-908)-10aaLinker-mCherry-Kras4BCT,phyB |
| 物种来源: | A. thaliana (mustard weed) |
| 插入大小: | 3542 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Kpn1(未破坏) |
| 3' 克隆位点: | Not1 (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pAL149 was a gift from Christopher Voigt (Addgene plasmid # 22275 ; http://n2t.net/addgene:22275 ; RRID:Addgene_22275) |
| 参考文献部分: | Spatiotemporal control of cell signalling using a light-switchable protein interaction. Levskaya A, Weiner OD, Lim WA, Voigt CA. Nature. 2009 Sep 13. ():. 10.1038/nature08446 PubMed 19749742 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAL149 8910 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAL149
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8910)
AUTHORS Levskaya A, Weiner OD, Lim WA, Voigt CA
TITLE Spatiotemporal control of cell signalling using a light-switchable
protein interaction.
JOURNAL Nature. 2009 Sep 13. ():.
PUBMED 19749742
REFERENCE 2 (bases 1 to 8910)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8910
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3667..4374
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(3812..3830)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 4080..4099
/label=mCherry-F
/note="mCherry, forward primer"
promoter complement(4463..4481)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4464..4481)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4501..4518)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4507..4731
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4777..5205
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4864..4883)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5074..5095
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5214..5234)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5219..5548
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5399..5534
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5461..5480
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5615..6409
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5669..5688)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6279..6298
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6583..6704
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6620..6639)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6674..6693
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6753..6769)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6753..6769)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6766..6788)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6777..6793
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6801..6831)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6846..6867
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6984..7001)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7155..7743)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7235..7254)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7914..8774)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8537..8556
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8775..8879)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 atggtttccg gagtcggggg tagtggcggt ggccgtggcg gtggccgtgg cggagaagaa
961 gaaccgtcgt caagtcacac tcctaataac cgaagaggag gagaacaagc tcaatcgtcg
1021 ggaacgaaat ctctcagacc aagaagcaac actgaatcaa tgagcaaagc aattcaacag
1081 tacaccgtcg acgcaagact ccacgccgtt ttcgaacaat ccggcgaatc agggaaatca
1141 ttcgactact cacaatcact caaaacgacg acgtacggtt cctctgtacc tgagcaacag
1201 atcacagctt atctctctcg aatccagcga ggtggttaca ttcagccttt cggatgtatg
1261 atcgccgtcg atgaatccag tttccggatc atcggttaca gtgaaaacgc cagagaaatg
1321 ttagggatta tgcctcaatc tgttcctact cttgagaaac ctgagattct agctatggga
1381 actgatgtga gatctttgtt cacttcttcg agctcgattc tactcgagcg tgctttcgtt
1441 gctcgagaga ttaccttgtt aaatccggtt tggatccatt ccaagaatac tggtaaaccg
1501 ttttacgcca ttcttcatag gattgatgtt ggtgttgtta ttgatttaga gccagctaga
1561 actgaagatc ctgcgctttc tattgctggt gctgttcaat cgcagaaact cgcggttcgt
1621 gcgatttctc agttacaggc tcttcctggt ggagatatta agcttttgtg tgacactgtc
1681 gtggaaagtg tgagggactt gactggttat gatcgtgtta tggtttataa gtttcatgaa
1741 gatgagcatg gagaagttgt agctgagagt aaacgagacg atttagagcc ttatattgga
1801 ctgcattatc ctgctactga tattcctcaa gcgtcaaggt tcttgtttaa gcagaaccgt
1861 gtccgaatga tagtagattg caatgccaca cctgttcttg tggtccagga cgataggcta
1921 actcagtcta tgtgcttggt tggttctact cttagggctc ctcatggttg tcactctcag
1981 tatatggcta acatgggatc tattgcgtct ttagcaatgg cggttataat caatggaaat
2041 gaagatgatg ggagcaatgt agctagtgga agaagctcga tgaggctttg gggtttggtt
2101 gtttgccatc acacttcttc tcgctgcata ccgtttccgc taaggtatgc ttgtgagttt
2161 ttgatgcagg ctttcggttt acagttaaac atggaattgc agttagcttt gcaaatgtca
2221 gagaaacgcg ttttgagaac gcagacactg ttatgtgata tgcttctgcg tgactcgcct
2281 gctggaattg ttacacagag tcccagtatc atggacttag tgaaatgtga cggtgcagca
2341 tttctttacc acgggaagta ttacccgttg ggtgttgctc ctagtgaagt tcagataaaa
2401 gatgttgtgg agtggttgct tgcgaatcat gcggattcaa ccggattaag cactgatagt
2461 ttaggcgatg cggggtatcc cggtgcagct gcgttagggg atgctgtgtg cggtatggca
2521 gttgcatata tcacaaaaag agactttctt ttttggtttc gatctcacac tgcgaaagaa
2581 atcaaatggg gaggcgctaa gcatcatccg gaggataaag atgatgggca acgaatgcat
2641 cctcgttcgt cctttcaggc ttttcttgaa gttgttaaga gccggagtca gccatgggaa
2701 actgcggaaa tggatgcgat tcactcgctc cagcttattc tgagagactc ttttaaagaa
2761 tctgaggcgg ctatgaactc taaagttgtg gatggtgtgg ttcagccatg tagggatatg
2821 gcgggggaac aggggattga tgagttaggt gcagttgcaa gagagatggt taggctcatt
2881 gagactgcaa ctgttcctat attcgctgtg gatgccggag gctgcatcaa tggatggaac
2941 gctaagattg cagagttgac aggtctctca gttgaagaag ctatggggaa gtctctggtt
3001 tctgatttaa tatacaaaga gaatgaagca actgtcaata agcttctttc tcgtgctttg
3061 agaggggacg aggaaaagaa tgtggaggtt aagctgaaaa ctttcagccc cgaactacaa
3121 gggaaagcag tttttgtggt tgtgaatgct tgttccagca aggactactt gaacaacatt
3181 gtcggcgttt gttttgttgg acaagacgtt actagtcaga aaatcgtaat ggataagttc
3241 atcaacatac aaggagatta caaggctatt gtacatagcc caaaccctct aatcccgcca
3301 atttttgctg ctgacgagaa cacgtgctgc ctggaatgga acatggcgat ggaaaagctt
3361 acgggttggt ctcgcagtga agtgattggg aaaatgattg tcggggaagt gtttgggagc
3421 tgttgcatgc taaagggtcc tgatgcttta accaagttca tgattgtatt gcataatgcg
3481 attggtggcc aagatacgga taagttccct ttcccattct ttgaccgcaa tgggaagttt
3541 gttcaggctc tattgactgc aaacaagcgg gttagcctcg agggaaaggt tattggggct
3601 ttctgtttct tgcaaatccc gagcgaattc gatagtgctg gtagtgctgg tagtgctggt
3661 actagtatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
3721 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
3781 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
3841 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
3901 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
3961 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
4021 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
4081 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
4141 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
4201 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
4261 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
4321 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggctagc
4381 ggtaaaaaga agaaaaagaa gtcaaagaca aagtgtgtaa ttatgtaagc ggccgctcga
4441 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
4501 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
4561 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
4621 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
4681 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
4741 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
4801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
4861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
4921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
4981 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
5041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
5101 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
5161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
5221 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
5281 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
5341 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
5401 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
5461 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
5521 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
5581 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
5641 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
5701 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
5761 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
5821 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
5881 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
5941 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
6001 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
6061 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
6121 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
6181 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
6241 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
6301 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
6361 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
6421 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
6481 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
6541 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
6601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
6661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
6721 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
6781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
6841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
6901 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
6961 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
7021 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
7081 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
7141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
7201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
7261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
7321 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
7381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
7441 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
7501 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
7561 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
7621 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
7681 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
7741 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
7801 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
7861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
7921 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
7981 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
8041 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
8101 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
8161 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
8221 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
8281 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
8341 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
8401 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
8461 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
8521 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
8581 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
8641 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
8701 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
8761 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
8821 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
8881 acatttcccc gaaaagtgcc acctgacgtc
//
