| 质粒名称: | pT7-VP3SA11 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 89164 |
| 存储实验室: | Takeshi Kobayashi |
|
载体骨架基本信息 |
|
| 载体名称: | p3E5 |
| 载体出品公司: | -- |
| 空载体大小: | 3074 bp |
| 完整质粒大小: | 5665 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rotavirus SA11 VP3 |
| 物种来源: | Rotavirus SA11 |
| 插入大小: | 2591 bp |
| 基因突变: | -- |
| GenBank编号 | LC178566 |
| 启动子: | T7 promotor |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 forward CTGTGGATAACCGTATTACCG |
| 3’测序引物 | T7 terminal GCTAGTTATTGCTCAGCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pT7-VP3SA11 was a gift from Takeshi Kobayashi (Addgene plasmid # 89164 ; http://n2t.net/addgene:89164 ; RRID:Addgene_89164) |
| 参考文献部分: | Entirely plasmid-based reverse genetics system for rotaviruses. Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T. Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 10.1073/pnas.1618424114. 10.1073/pnas.1618424114 PubMed 28137864 |
买家导航
pT7VP3SA11载体基本信息
pT7VP3SA11载体图谱质粒图谱和多克隆位点信息
pT7VP3SA11载体简介
pT7VP3SA11载体序列
LOCUS pT7-VP3SA11 5664 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express Rotavirus SA11 VP3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pT7-VP3SA11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5664)
AUTHORS Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M,
Matsuura Y, Taniguchi K, Kobayashi T
TITLE Entirely plasmid-based reverse genetics system for rotaviruses.
JOURNAL Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi:
10.1073/pnas.1618424114.
PUBMED 28137864
REFERENCE 2 (bases 1 to 5664)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5664
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(167..755)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(247..266)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(926..1786)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1549..1568
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1787..1891)
/gene="bla"
/label=AmpR promoter
primer_bind 1959..1977
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2015..2037)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2137..2156
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2366..2385
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 2675..2693
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2675..2692
/label=SP6
/note="SP6 promoter, forward primer"
terminator 2761..2808
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 2804..2822
/label=T7 Term
/note="T7 terminator, reverse primer"
promoter complement(5558..5576)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5660..13)
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
61 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
361 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
601 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
1021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
1081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
1141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
1201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
1261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
1321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
1381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
1441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
1501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
1561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
1621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
1681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
1741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
1801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
1861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
1921 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
1981 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
2041 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
2101 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
2161 gagtgcacca ttcgacgctc tcccttatgc gactcctgca ttaagaagca gcccagtagt
2221 aggttgaggc cgttgagcac cgccgccgca aggaatggtg caaggagatg gcgcccaaca
2281 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
2341 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
2401 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg ctagcgatga
2461 ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca gaaactcaga
2521 aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct
2581 tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca
2641 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaatacaag
2701 cttgcatgcc tgcaggtcga ctctagagga tctcgatccg gatatagttc ctcctttcag
2761 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctagtt attgctcagc
2821 ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat cctttttttt
2881 gagctctccc ttagccatcc gagtggacga cgtcctcctt cggatgccca ggtcggaccg
2941 cgaggaggtg gagatgccat gccgacccgg tcacatcatg actagtgtgt taagttttta
3001 gctcactcaa ccatatcaaa cactgtatca cccttaatct ttcttaaata aatggattta
3061 atttgtaatt tagtagtctc tatccttact gttctcatgt tattgaagct taatgttatg
3121 tgcggaataa acattctgga ttggccaaaa gttatttgat ttttataaaa tacgttatag
3181 attttaaagt atgtagataa tactccacta acatttgcta cgtagattcc atctgataac
3241 atatatgtat aactggtcgt aatatccatt ttaaataatt cattcttcat tatctttagt
3301 atgtcaatca cgtctggcac catgttatca gaaatatgta atactccgaa ggttaaatgc
3361 ggtattttaa catcatattt atatggtata tttctaaatt gtattccgat aaagtaattc
3421 ggatcatcag cgtatgttat actatatact gtattaatat aattctctat ttcgtttgaa
3481 tatctcaata cagttaagtc atcctgcaat gtagcaaatt cttttgctga tctacatgca
3541 taaattaatt ctattggtgc gtgttcataa tactttcctc cttctatatc aactttatct
3601 atagaatgta aataaatcca gcgtttaaga tcaaatgaat aattatatct ataataaatt
3661 aaagcattat atacatgacc tgatactgaa ttctctatat tagtaccaat tagattaaaa
3721 ttattattgg acaaatctct aaatatataa cctgaatatg aagtagcaga ttccgaaaac
3781 ctaatttggt gtgatcttct cgatatactg gtgtgattag caaattgatc caatttataa
3841 tacttatctg taccacttaa aatgaataaa tgattattcc ctgttggttt tgatgctaac
3901 gatatagaca aaccaaagag tcctaaacaa ccgtcgtatg aagtaattat agcttctttg
3961 gtatcaaagt cggttggaag aaaggtccaa tcatagatat ttttgaaccc aacttttggt
4021 tcatccttaa acgtattatt tattctaaca gttattagag attgtttctg attattaatt
4081 aatttaatta cttctgatct attattaaaa tcatttgata atgcatataa cgctacaata
4141 taactatcat tcagtacttt tactttaaat ggttgttgaa taaacacatt ttcagttatt
4201 atattgttta taaatgaata taacacgcct ttaggaatga acctcctaat gttagttaaa
4261 tcccaagtat ctagtaataa ataaaattct gatcttattt ccgtagttgg gtggtgcagt
4321 aatttagctg aaattggcag ttccaaatcc atagctgtca tcttaacaca acacaccttc
4381 gctttacccg ttcgtaaata ttcataagct atgtccaaat tattaatagt ttgttcttct
4441 actgtctttc tccatttttt ccaatcagca ttgcccctat cggttcttat atcaatatac
4501 aatattgagt tatccttcag tttcttcaca tcatttataa aaaataattc tttgtgatgg
4561 attgatgaat atggtgtgtc caatggatcc caagtttcaa agaaaaaatc ggaataatgc
4621 ctaacgtcat atatccaata agatggagca gatccgatca tatatataat tggtttactt
4681 ttatataaat tatagtaata ataaattgct gtaagaacat tcagcatatt tttatactga
4741 ccaatatcaa actgagatat ccttttacct aaccatcgtt cttgttttaa ttttatagta
4801 tcttttacta aatacgcacc atttgaaaat tgttttatag ttggataatt taaaaatgta
4861 aacatagatt cattacgcgg accaacgttc actacattct tatgttcttt caataatgat
4921 gggaataact tatccctaaa tactctgtta gataatctag ctataggcaa cgatgtagtc
4981 attctttcct ttatcttttt taacataaaa ttttggaagt ccaacgcttc tgaataaatg
5041 tattcaatta ttagatcatc atctgttgca gcgttttgac atttaaatga aaagttataa
5101 tgtcctctag tttcacttcc attataacca acatatgtta atttccatcc aggtagtaaa
5161 tattcttcaa tatttgtttt tgttatccaa tgttggtctg ttctaatttc attattggta
5221 taatatgaat aatcatgtaa atatattata tcaagttcat aatcataagt gaaattacac
5281 cttattaatg tgaataattc ttcaagtgat tgtaaagcaa ttgcagctat tcctgactta
5341 tttaatattt ctaatgtttt aatgctataa tttaagtata aaatattatg ggtcgtaaga
5401 ttagagatta aaaaagcgtt ttcataacta tcttttgtat catcatgaac gtagacttga
5461 gtgtctgcat acacttgagc cacactgtgt cttaaagcta gtactttcat gtttacacca
5521 tcagaggtaa aacacactac tggtactgct ttaatagcct atagtgagtc gtattaaccg
5581 ggatcgatat ccccctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
5641 ttgggcgctc ttccgcttcc tcgc
//
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