pAAVLINK-EF1a-HA-5'-ARID2

LOCUS       pAAVLINK-EF1a-HA-5'-ARID2                7067 bp DNA     circular SYN 12-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..4229
                     /codon_start=1
                     /label=ARID2
                     /translation="ANSTGKAPPDERRKGLAFLDELRQFHHSRGSPFKKIPAVGGKELD
                     LHGLYTRVTTLGGFAKVSEKNQWGEIVEEFNFPRSCSNAAFALKQYYLRYLEKYEKVHH
                     FGEDDDEVPPGNPKPQLPIGAIPSSYNYQQHSVSDYLRQSYGLSMDFNSPNDYNKLVLS
                     LLSGLPNEVDFAINVCTLLSNESKHVMQLEKDPKIITLLLANAGVFDDTLGSFSTVFGE
                     EWKEKTDRDFVKFWKDIVDDNEVRDLISDRNKSHEGTSGEWIWESLFHPPRKLGINDIE
                     GQRVLQIAVILRNLSFEEGNVKLLAANRTCLRFLLLSAHSHFISLRQLGLDTLGNIAAE
                     LLLDPVDFKTTHLMFHTVTKCLMSRDRFLKMRGMEILGNLCKAEDNGVLICEYVDQDSY
                     REIICHLTLPDVLLVISTLEVLYMLTEMGDVACTKIAKVEKSIDMLVCLVSMDIQMFGP
                     DALAAVKLIEHPSSSHQMLSEIRPQAIEQVQTQTHVASAPASRAVVAQHVAPPPGIVEI
                     DSEKFACQWLNAHFEVNPDCSVSRAEMYSEYLSTCSKLARGGILTSTGFYKCLRTVFPN
                     HTVKRVEDSSSNGQAHIHVVGVKRRAIPLPIQMYYQQQPVSTSVVRVDSVPDVSPAPSP
                     AGIPHGSQTIGNHFQRTPVANQSSNLTATQMSFPVQGVHTVAQTVSRIPQNPSPHTHQQ
                     QNAPVTVIQSKAPIPCEVVKATVIQNSIPQTGVPVSIAVGGGPPQSSVVQNHSTGPQPV
                     TVVNSQTLLHHPSVIPQQSPLHTVVPGQIPSGTPVTVIQQAVPQSHMFGRVQNIPACTS
                     TVSQGQQLITTSPQPVQTSSQQTSAGSQSQDTVIIAPPQYVTTSASNIVSATSVQNFQV
                     ATGQMVTIAGVPSPQASRVGFQNIAPKPLPSQQVSSTVVQQPIQQPQQPTQQSVVIVSQ
                     PAQQGQTYAPAIHQIVLANPVALPAGQTVQLTGQPNITPSSSPSPVPATNNQVPTAMSS
                     SSTPQSQGPPPTVSQMLSVKRQQQQQHSPAPPPQQVQVQVQQPQQVQMQVQPQQSNAGV
                     GQPASGESSLIKQLLLPKRGPSTPGGKLILPAPQIPPPNNARAPSPQVVYQVASNQAAG
                     FGVQGQTPAQQLLVGQQNVQLVPSAMPPSGGVQTVPISNLQILPGPLISNSPATIFQ"
     misc_feature    1629..1634
                     /label=SDS
     misc_feature    2938..2943
                     /label=SDS
     misc_feature    3731..3736
                     /label=SDS
     intron          4230..4270
                     /label=Splicing Donor
     misc_feature    4274..4307
                     /label=lox2272-JT1571
     repeat_region   4352..4470
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4352..4459
                     /label=AAV2 ITR
     rep_origin      4545..5000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5282..5386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5387..6247
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6418..7006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctgcaa actcgacggg gaaggcgcct ccggacgagc ggagaaaggg
      781 actcgctttc ctggacgagc tgcggcagtt ccaccacagc agagggtcgc cttttaaaaa
      841 aatccctgcg gtgggtggga aggagctgga tcttcacggt ctctacacca gagtcactac
      901 tttaggcgga ttcgcgaagg tttctgagaa gaatcagtgg ggagaaattg ttgaagagtt
      961 caactttccc agaagttgtt ctaacgctgc ctttgcttta aaacagtatt acttgcgtta
     1021 cctagaaaag tacgagaaag ttcatcattt tggggaggat gatgatgagg taccaccagg
     1081 caatccaaag ccacagcttc ctattggtgc aattccatct tcctacaatt accagcaaca
     1141 cagtgtgtcg gattatctgc gtcaaagtta tgggctgtcc atggacttta attcgccaaa
     1201 tgattataat aaattggtgc tttcactgtt atctggactc ccaaatgaag tggactttgc
     1261 tattaacgta tgcactctcc tatcaaatga aagcaagcac gtcatgcaac ttgaaaaaga
     1321 tcctaaaatc atcactttac tacttgctaa tgccggggtg tttgacgaca ctttaggatc
     1381 cttttccact gtatttggag aagaatggaa agagaagact gatagagact tcgttaagtt
     1441 ttggaaagac atcgttgatg ataatgaagt tcgtgacctc atttctgaca gaaacaagtc
     1501 tcatgaaggt acatcaggag aatggatttg ggagtcttta tttcatccac ctcgaaagct
     1561 gggcattaac gatattgaag gacagcgggt acttcagatt gcagtgattt tgagaaatct
     1621 ttcctttgag gagggcaatg ttaagctctt ggcagctaat cgtacctgtc ttcgtttcct
     1681 attactttct gcacatagtc attttatttc tttaaggcaa ttaggccttg acacattagg
     1741 aaatattgca gctgagcttt tactggaccc tgttgatttc aaaactactc atctgatgtt
     1801 tcatactgtt acaaaatgtc taatgtcaag ggatagattt ttaaagatga gaggcatgga
     1861 aattttggga aatctttgca aagcagaaga taatggtgtt ttaatttgtg aatatgtgga
     1921 tcaggattcc tacagagaga tcatttgtca tctcacttta cctgatgtgc tgcttgtaat
     1981 ctcaacactc gaggtgctat acatgctcac ggaaatggga gatgttgctt gcacaaaaat
     2041 tgcaaaagta gaaaagagca tagacatgtt agtgtgtctg gtttctatgg atattcagat
     2101 gtttggccct gatgcactag ctgcggtaaa actcattgaa cacccaagtt ccagtcatca
     2161 aatgttatct gaaattaggc cacaagctat agagcaagtc caaacccaga ctcatgtagc
     2221 atctgcccca gcttccagag cagttgtagc gcagcatgtt gctccacctc caggaatagt
     2281 ggaaatagat agtgagaagt ttgcttgtca gtggctaaat gctcattttg aagtaaatcc
     2341 agattgttct gtttctcgag cagaaatgta ttctgaatac ctctcgactt gcagtaaatt
     2401 agctcgtggt ggaatcctaa catcaactgg attttataaa tgtcttagaa cggtctttcc
     2461 aaatcataca gtgaagagag tggaggattc cagtagcaat gggcaggcac atattcatgt
     2521 ggtaggagta aaacggaggg ctataccact tcccattcag atgtactatc agcagcaacc
     2581 agtttctact tctgttgttc gtgttgattc tgttcctgat gtatctcctg ctccttcacc
     2641 tgcaggaatc cctcatggat cacaaaccat aggaaaccat tttcagagga ctcctgttgc
     2701 caaccaatct tcaaatctga ctgcaacaca aatgtctttt cctgtacaag gtgttcatac
     2761 tgtggcacaa actgtttcaa gaattccaca aaatccttca cctcataccc accagcaaca
     2821 aaatgctcca gtgactgtca ttcaaagtaa agctccaatt ccttgtgaag ttgttaaggc
     2881 tacagttatc cagaattcca taccccagac aggagttcct gttagtattg ctgttggagg
     2941 aggacctcca cagagttctg ttgttcagaa tcatagtaca gggccacaac ctgttacagt
     3001 tgtgaattct cagacattgc ttcaccatcc atctgtaatt ccacagcagt ctccattaca
     3061 cacagtggta ccaggacaga tcccttcagg cactcctgtt acagtaattc aacaagctgt
     3121 cccacagagt catatgtttg gcagagtaca gaacatacca gcatgtactt ctacagtttc
     3181 acagggtcaa cagttaatca ccacatcacc ccaacctgtg caaacttcat ctcaacagac
     3241 atcagctggt agccagtcac aagatactgt tatcatagca cccccacagt atgtaacaac
     3301 ttctgcatcc aatattgtct cagcaacttc agtacagaat tttcaggtag ctacaggaca
     3361 aatggttact attgctggtg tcccaagtcc acaagcctca agggtagggt ttcagaacat
     3421 tgcaccaaaa cctctccctt ctcagcaagt ttcatctaca gtggtacagc agcctattca
     3481 acaaccacag cagccaaccc aacaaagcgt agtgattgta agccagccag ctcaacaagg
     3541 tcaaacttat gcaccagcca ttcaccaaat tgttcttgct aatccagtag ctcttccagc
     3601 tggtcagaca gttcagctaa ctggacaacc taacataact ccatcttctt caccatcacc
     3661 tgtcccagct actaataacc aagtccctac tgccatgtcg tcgtcctcta cccctcaatc
     3721 acagggacca cctcctactg tcagtcaaat gttatctgtg aaaaggcagc aacagcagca
     3781 acattcacca gcacccccac cacagcaggt acaagtacaa gttcagcagc cccaacaagt
     3841 acagatgcaa gttcaacctc aacagtcgaa tgcaggagtt ggtcagcctg cctctggtga
     3901 gtcgagtctg attaaacagc ttctgcttcc gaaacgtggt ccttcaacac caggtggtaa
     3961 gcttattctc ccagctccac agattcctcc ccctaataat gcaagagctc ctagccctca
     4021 ggtggtctat caggtggcca gtaaccaagc cgcaggtttt ggagtgcagg ggcaaactcc
     4081 agctcagcag ctattggttg ggcagcaaaa tgttcagttg gtcccaagtg caatgccacc
     4141 ctcaggggga gtacaaactg tgcccatttc gaacttacaa atattgccag gtccactgat
     4201 ctcaaatagc ccagcaacca ttttccaagg taagtatcaa ggttacaaga caggtttaag
     4261 gagaccaata agctattatt cgtataggat actttatacg aagttattaa gttatggaga
     4321 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4381 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccggcctcag
     4441 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     4501 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4561 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4621 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4681 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4741 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4801 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4861 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     4921 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4981 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     5041 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5101 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5161 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//