| 出品公司: | Clontech |
|---|---|
| 载体名称: | pCMV-(DYKDDDDK)-C |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: |
3766 dp
|
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | FLAG C-term |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: |
-- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 哺乳动物细胞中表达C-末端标记蛋白的载体。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
-C载体图谱.png)
载体简介
载体序列
LOCUS pCMV-(DYKDDDDK)-C 3766 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for expressing a C-terminally FLAG-tagged protein in
mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-(DYKDDDDK)-C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3766)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, November 30, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3766
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
misc_feature 820..863
/note="MCS"
/note="multiple cloning site"
CDS 867..890
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
polyA_signal 905..1039
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1150..1166)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1174..1190
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1198..1228)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(1552..2140)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2311..3171)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3172..3276)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt
841 cgaccgagat ctctcgaggt accggcgact acaaggacga cgatgacaag taagcggccg
901 cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
961 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
1021 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
1081 ggaggtgtgg gaggtttttt cggatcctct agagtcgatc tgcaggcatg ctagcttggc
1141 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
1201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
1261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
1321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
1381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
1441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
1501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
1561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
1621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
1681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
1741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
1801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
1861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
1921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
1981 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
2041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
2101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
2161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
2221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
2281 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
2341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
2401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
2461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
2521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
2581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
2641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
2701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
2761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
2821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
2881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
2941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
3001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
3061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
3121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
3181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
3241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
3301 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
3361 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
3421 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
3481 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
3541 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
3601 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
3661 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
3721 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagt
//
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