pBTB-2

价格:3000元

联系方式:I47-825O-882O

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载体基本信息

出品人: Lynch,M.D. and Gill,R.T.
载体名称: pBTB-2
质粒类型: 广宿主载体;克隆载体;原核表达载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: araBAD
载体大小: 3740 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
克隆菌株: DH5α 等
宿主细胞(系): 多个菌属
备注: --
产品目录号: --
组成型/诱导型: 诱导型

载体质粒图谱和多克隆位点信息

pBTB-2载体图谱



载体简介

载体序列

LOCUS       DQ058730                3740 bp    DNA     circular SYN 06-FEB-2009
DEFINITION  Broad host range vector pBTB-2, complete sequence.
ACCESSION   DQ058730
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
REFERENCE   3  (bases 1 to 3740)
  AUTHORS   Godiska,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2009) Lucigen Corp., 2120 W. Greenview Dr., #9,
            Middleton, WI 53562, USA
  REMARK    Sequence update by submitter
COMMENT     On Feb 6, 2009 this sequence version replaced gi:69048318.
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Broad host range vector pBTB-2"
                     /mol_type="other DNA"
                     /db_xref="taxon:334962"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            872..1534
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             872..1534
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99720.1"
                     /db_xref="GI:69048319"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            1631..2446
                     /gene="kan"
                     /note="kanamycin resistance gene from TN903"
     CDS             1631..2446
                     /gene="kan"
                     /codon_start=1
                     /transl_table=11
                     /product="kanamycin kinase"
                     /protein_id="AAY99721.1"
                     /db_xref="GI:69048320"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGL
                     VGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     regulatory      2463..2494
                     /regulatory_class="terminator"
                     /note="TonB terminator"
     gene            complement(2522..3451)
                     /gene="araC"
     CDS             complement(2522..3451)
                     /gene="araC"
                     /note="regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC"
                     /protein_id="AAY99722.1"
                     /db_xref="GI:69048321"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     regulatory      3459..3726
                     /regulatory_class="promoter"
                     /note="pBAD promoter"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
     1681 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1741 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1801 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga
     1861 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1921 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1981 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2041 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2101 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2161 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
     2221 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2281 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     2341 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     2401 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctgagggc ggatccccct
     2461 caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt
     2521 tttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
     2581 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
     2641 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
     2701 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
     2761 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
     2821 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg
     2881 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
     2941 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
     3001 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa
     3061 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
     3121 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga
     3181 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct
     3241 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
     3301 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
     3361 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
     3421 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
     3481 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
     3541 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
     3601 acattgatta tttgcacggc gtcacacttt gctatgccat aacattttta tccataagat
     3661 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
     3721 tttgggctag agaattcgtc
//

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