pAAVLINK-EF1a-5'-ADGRL1

LOCUS       pAAVLINK-EF1a-5'-ADGRL1              5489 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     regulatory      707..716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             713..2629
                     /codon_start=1
                     /label=ADGRL1
                     /translation="MARLAAVLWNLCVTAVLVTSATQGLSRAGLPFGLMRRELACEGYP
                     IELRCPGSDVIMVENANYGRTDDKICDADPFQMENVQCYLPDAFKIMSQRCNNRTQCVV
                     VAGSDAFPDPCPGTYKYLEVQYDCVPYIFVCPGTLQKVLEPTSTHESEHQSGAWCKDPL
                     QAGDRIYVMPWIPYRTDTLTEYASWEDYVAARHTTTYRLPNRVDGTGFVVYDGAVFYNK
                     ERTRNIVKYDLRTRIKSGETVINTANYHDTSPYRWGGKTDIDLAVDENGLWVIYATEGN
                     NGRLVVSQLNPYTLRFEGTWETGYDKRSASNAFMVCGVLYVLRSVYVDDDSEAAGNRVD
                     YAFNTNANREEPVSLTFPNPYQFISSVDYNPRDNQLYVWNNYFVVRYSLEFGPPDPSAG
                     PATSPPLSTTTTARPTPLTSTASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPVPS
                     TRRPPAPNLHVSPELFCEPREVRRVQWPATQQGMLVERPCPKGTRGIASFQCLPALGLW
                     NPRGPDLSNCTSPWVNQVAQKIKSGENAANIASELARHTRGSIYAGDVSSSVKLMEQLL
                     DILDAQLQALRPIERESAGKNYNKMHKRERTCKDYIKAVVETVDNLLRPEALESWKDMN
                     ATEQ"
     misc_feature    2229..2234
                     /label=SDS
     misc_feature    2409..2414
                     /label=SDS
     intron          2630..2670
                     /label=Splicing Donor
     misc_feature    2674..2707
                     /label=lox2272-JT1571
     repeat_region   2752..2892
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    2752..2881
                     /label=AAV2 ITR
     rep_origin      2967..3422
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3704..3808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3809..4669
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4840..5428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatggcccg
      721 cctagccgca gtgctctgga atctgtgtgt caccgccgtc ctggtcacct cggccaccca
      781 aggcctgagc cgggccgggc tcccgttcgg gctgatgcgc cgggagctgg cgtgtgaagg
      841 ctaccccatc gagctgcggt gccccggcag cgacgtcatc atggtggaga atgccaacta
      901 cgggcgcacg gacgacaaga tttgcgatgc tgaccctttc cagatggaga atgtgcagtg
      961 ctacctgccg gacgccttca agatcatgtc acagaggtgt aacaaccgca cccagtgcgt
     1021 ggtggtcgcc ggctcggatg cctttcctga cccctgtcct gggacctaca agtacctgga
     1081 ggtgcagtac gactgtgtcc cctacatctt cgtgtgccca gggaccctgc agaaggtgct
     1141 ggagcccacc tcgacacacg agtcagagca ccagtctggc gcatggtgca aggacccgct
     1201 gcaggcgggt gaccgcatct acgtgatgcc ctggatcccc taccgcacgg acacactgac
     1261 tgagtatgcc tcgtgggagg actacgtggc cgcccgccac accaccacct accgcctgcc
     1321 caaccgcgtg gatggcacag gctttgtggt ctacgatggt gccgtcttct acaacaagga
     1381 gcgcacgcgc aacatcgtca agtatgacct acggacgcgc atcaagagcg gggagacggt
     1441 catcaatacc gccaactacc atgacacctc gccctaccgc tggggcggaa agaccgacat
     1501 tgacctggcg gtggacgaga acgggctgtg ggtcatctac gccactgagg gcaacaacgg
     1561 gcggctggtg gtgagccagc tgaaccccta cacactgcgc tttgagggca cgtgggagac
     1621 gggttacgac aagcgctcgg catccaacgc cttcatggtg tgtggggtcc tgtacgtcct
     1681 gcgttccgtg tacgtggatg atgacagcga ggcggctggc aaccgcgtgg actatgcctt
     1741 caacaccaat gccaaccgcg aggagcctgt cagcctcacc ttccccaacc cctaccagtt
     1801 catctcctcc gttgactaca accctcgcga caaccagctg tacgtctgga acaactattt
     1861 cgtggtgcgc tacagcctgg agttcgggcc gcccgacccc agtgctggcc cagccacttc
     1921 cccacccctc agcacgacca ccacagccag gcccacgccc ctcaccagca cagcctcgcc
     1981 cgcagccacc accccgctcc gccgggcacc cctcaccacg cacccagtgg gtgccatcaa
     2041 ccagctggga cctgatctgc ctccagccac agccccagtc cccagcaccc ggcggccccc
     2101 agccccgaat ctacacgtgt cccctgagct cttctgcgag ccccgagagg tacggcgggt
     2161 ccagtggccg gccacccagc agggcatgct ggtggagagg ccctgcccca aggggactcg
     2221 aggaattgcc tccttccagt gtctaccagc cttggggctc tggaaccccc ggggccctga
     2281 cctcagcaac tgcacctccc cctgggtcaa ccaggtggcc cagaagatca agagtgggga
     2341 gaacgcggcc aacatcgcca gcgagctggc ccgacacacc cggggctcca tctacgcggg
     2401 ggacgtctcc tcctctgtga agctgatgga gcagctgctg gacatcctgg atgcccagct
     2461 gcaggccctg cggcccatcg agcgcgagtc agccggcaag aactacaaca agatgcacaa
     2521 gcgagagaga acttgtaagg attatatcaa ggccgtggtg gagacagtgg acaatctgct
     2581 ccggccagaa gctctggagt cctggaagga catgaatgcc acggagcagg taagtatcaa
     2641 ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat actttatacg
     2701 aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
     2761 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     2821 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     2881 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2941 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     3001 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     3061 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3121 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3181 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3241 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     3301 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     3361 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3421 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3481 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3541 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3601 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3661 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3781 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3841 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3901 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3961 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4021 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4081 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4141 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4201 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4261 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4321 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4381 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4441 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4501 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4561 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4621 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4681 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4741 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4981 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5461 ggccttttgc tggccttttg ctcacatgt
//