pFB-hrGFP

价格:3000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Agilent
载体名称: pFB-hrGFP
质粒类型: 逆病毒载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 5838 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: DH5α 等
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 逆病毒载体pFB-hrGFP组成型表达hrGFP绿色荧光蛋白;hrGFP细胞毒性低
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pFB-hrGFP载体图谱



载体简介

pFB-hrGFP载体限制性酶切位点

pFB-hrGFP, 5838 bp			       version 013001


Enzymes with 1-10 cleavage sites:
           
              #sites   --  Bp position of recognition site --

  AatII          2      978,   5764
  Acc65I         3      647,   1846,   3364
  AccI           3     1967,   2057,   3724
  AflII          3      202,   1265,   2919
  AflIII         3      164,   2157,   3953
  AgeI           1     1840
  AhdI           6      687,    733,   1274,   3404,   3450
                       4841
  Alw44I         4     1054,   3769,   4267,   5513
  AlwNI          6      323,    398,   2084,   3040,   3115
                       4364
  ApaI           1     1238
  ApoI           3       87,   1128,   2063
  BanII          8      580,    593,    764,   1238,   2266
                       3297,   3310,   3481
  BbeI           4      615,   1656,   2234,   3332
  BbsI           3     2494,   2686,   5831
  BbvCI          2      453,   3170
  BciVI          5      656,   1986,   3373,   4162,   5689
  BglI           1     4960
  BglII          1     1678
  BpmI           5     1799,   2135,   2681,   2867,   4931
  Bpu10I         8      337,    412,    453,   1548,   2263
                       3054,   3129,   3170
  BsaAI          5     1816,   2000,   2156,   2693,   3705
  BsaHI          7      615,    978,   1656,   2234,   3332
                       5382,   5764
  BsaI          10      694,    715,    782,   1414,   1802
                       2726,   3411,   3432,   3499,   4913
  BsaWI          5      138,   1840,   4159,   4306,   5137
  BseRI          6      718,   1068,   1561,   1600,   2426
                       3435
  BsgI           2     2213,   2774
  BsiEI          8      961,   1034,   1838,   2801,   3866
                       4290,   5213,   5362
  BsiHKAI        7      580,   1054,   3297,   3769,   4267
                       5428,   5513
  BsmBI          6      976,   1093,   1337,   1396,   1582
                       3602
  Bsp120I        1     1238
  BspHI          4     2115,   4673,   5681,   5786
  BspLU11I       2      164,   3953
  BsrBI          5     1299,   2798,   2823,   3884,   5685
  BsrDI          2     4900,   5082
  BsrGI          2     1541,   2532
  BssHII         2      563,   3280
  BssSI          5     2723,   2777,   4126,   5510,   5817
  Bst1107I       1     3724
  BstAPI         5      322,    397,   3039,   3114,   3771
  BstEII         1     1346
  Bsu36I         1     1276
  Cfr10I         5     1840,   2099,   2348,   2504,   4926
  DraI           3     4710,   4729,   5421
  DraIII         1     1873
  DrdI           2     3642,   4055
  DsaI           4      151,    931,   2070,   2166
  EaeI           7      827,    961,   1368,   1389,   1668
                       2801,   5234
  EarI           5     1334,   1564,   2626,   3831,   5635
  Ecl136II       2      580,   3297
  Eco52I         2      961,   2801
  Eco57I         3     2090,   4480,   5528
  Eco72I         2     2000,   2156
  EcoNI          2     1647,   2419
  EcoO109I       5      499,   1477,   1924,   3216,   5821
  EcoRI          1     2063
  EcoRV          4      308,    383,   3025,   3100
  EheI           4      615,   1656,   2234,   3332
  FspI           2     2613,   5066
  HaeII          6      615,   1656,   2234,   3332,   3827
                       4197
  HgaI           6      889,   1166,   3659,   4055,   4633
                       5383
  HincII         2     2057,   5385
  HinfI         10      637,   1020,   1045,   1863,   2829
                       3354,   3853,   3928,   4324,   4841
  KasI           4      615,   1656,   2234,   3332
  KpnI           3      647,   1846,   3364
  MscI           3      827,   1368,   1668
  MslI           6      159,   2068,   2617,   5094,   5253
                       5612
  MunI           2       11,     21
  NaeI           1     2348
  NarI           4      615,   1656,   2234,   3332
  NcoI           2     2070,   2166
  NdeI           3     1664,   1672,   3775
  NgoMIV         1     2348
  NheI           5        6,     16,     26,    197,   2914
  NotI           1     2800
  NspI           4      164,   2610,   3588,   3953
  PleI           9      637,   1020,   1045,   1863,   2829
                       3354,   3853,   4324,   4841
  PpuMI          4      499,   1477,   1924,   3216
  PshAI          1     1015
  Psp1406I       2     5071,   5444
  PstI           5     1178,   1360,   2105,   2510,   5087
  PvuI           2     1034,   5213
  PvuII          7      286,    361,   2207,   2602,   2741
                       3003,   3078
  SacI           2      580,   3297
  SacII          1      151
  SalI           1     2057
  SapI           2     2626,   3830
  SbfI           1     2104
  ScaI           1     5324
  SchI           9      637,   1020,   1045,   1863,   2829
                       3354,   3853,   4324,   4841
  SexAI          1     1474
  SfcI          10      182,   1178,   1360,   2105,   2510
                       2599,   2899,   4218,   4409,   5087
  SmaI           4      643,   1241,   2018,   3360
  SmlI           8      202,   1265,   2795,   2919,   4059
                       4321,   4598,   5466
  SpeI           1      897
  SrfI           1     1240
  SspI           1     5648
  StyI           8      496,    706,   1504,   2070,   2166
                       2229,   3213,   3423
  TatI           5     1541,   2451,   2532,   3759,   5324
  TfiI           1     3928
  Tsp45I         7     1282,   1491,   2225,   3608,   3703
                       5103,   5314
  TspRI         10     1284,   1875,   3849,   4355,   4368
                       4639,   4788,   4893,   5240,   5267
  Tth111I        4      633,   1473,   3350,   3697
  VspI           1     5017
  XbaI           3      464,   2036,   3181
  XcmI           1     2517
  XhoI           1     2795
  XhoII         10     1678,   2085,   2217,   2400,   4594
                       4605,   4691,   4703,   5471,   5488
  XmaI           4      643,   1241,   2018,   3360
  XmnI           2     2644,   5441





Enzymes that do NOT cut molecule:

AccIII      AscI        BamHI       BclI        BlnI        
Bpu1102I    BsaBI       BsiWI       BsmI        BspMI       
BstBI       BstXI       ClaI        Eco47III    FseI        
HindIII     HpaI        MluI        NruI        NsiI        
PacI        PmeI        Ppu10I      RsrII       SanDI       
SfiI        SgfI        SgrAI       SnaBI       SphI        
StuI        SwaI        Van91I      

载体序列

载体质粒图谱pdf版和相关资料下载

载体应用举例

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