pCS2-

价格:2500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: --
载体名称: pCS2(-)
质粒类型: 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: simian CMV IE94、lac、T3、SP6
载体大小: 4095 bp
5' 测序引物及序列: SP6 fwd(MCS1)、T3 fwd(MCS2)
3' 测序引物及序列: EBV rev(MCS1)、SV40 polyA rev(MCS2)
载体标签:
载体抗性: 氨苄青霉素
克隆菌株: DH10B
表达细胞: 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。
备注: pCS2(-)载体能够在多种细胞中进行高水平的瞬表达。pCS2(-)载体同时也能进行体外转录和翻译。
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCS2(-)载体图谱



pCS2(-) 多克隆位点1

pCS2(-) 多克隆位点2

载体简介

载体序列

LOCUS       pCS2-	4095 bp 	DNA	SYN
DEFINITION  pCS2-
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCS2(-)|
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="pCS2-"
                     /mol_type="other DNA"
     misc_feature    35..52
                     /label="Sp6_primer"
     terminator      complement(154..273)
                     /label="SV40_PA_terminator"
     misc_feature    complement(168..187)
                     /label="EBV_rev_primer"
     promoter        complement(390..409)
                     /label="T3_promoter"
     promoter        complement(426..444)
                     /label="M13_reverse_primer"
     misc_feature    complement(443..465)
                     /label="M13_pUC_rev_primer"
     promoter        complement(479..508)
                     /label="lac_promoter"
     gene            complement(1591..2451)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1591..2451)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2493..2521)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2600..2906
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4013..4054
                     /label="CMV_fwd_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
   61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCA AGGCCTCTCG AGCCTCTAGA
  121 ACTATAGTGA GTCGTATTAC GTAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
  181 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
  241 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
  301 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA ATTCGCGGCC GCGGCGCCAA
  361 TGCATTGGGC CCGGTACCCA GCTTTTGTTC CCTTTAGTGA GGGTTAATTG CGCGCTTGGC
  421 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
  481 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
  541 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
  601 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC
  661 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
  721 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
  781 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
  841 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
  901 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
  961 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
 1021 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
 1081 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
 1141 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
 1201 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
 1261 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
 1321 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
 1381 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
 1441 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
 1501 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
 1561 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
 1621 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
 1681 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
 1741 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
 1801 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
 1861 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
 1921 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
 1981 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
 2041 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
 2101 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
 2161 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
 2221 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
 2281 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
 2341 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
 2401 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
 2461 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
 2521 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
 2581 TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC
 2641 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC
 2701 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT
 2761 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG
 2821 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG
 2881 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT
 2941 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT
 3001 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC ATTCGCCATT CAGGCTGCGC
 3061 AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGTC GACCATAGCC
 3121 AATTCAATAT GGCGTATATG GACTCATGCC AATTCAATAT GGTGGATCTG GACCTGTGCC
 3181 AATTCAATAT GGCGTATATG GACTCGTGCC AATTCAATAT GGTGGATCTG GACCCCAGCC
 3241 AATTCAATAT GGCGGACTTG GCACCATGCC AATTCAATAT GGCGGACTTG GCACTGTGCC
 3301 AACTGGGGAG GGGTCTACTT GGCACGGTGC CAAGTTTGAG GAGGGGTCTT GGCCCTGTGC
 3361 CAAGTCCGCC ATATTGAATT GGCATGGTGC CAATAATGGC GGCCATATTG GCTATATGCC
 3421 AGGATCAATA TATAGGCAAT ATCCAATATG GCCCTATGCC AATATGGCTA TTGGCCAGGT
 3481 TCAATACTAT GTATTGGCCC TATGCCATAT AGTATTCCAT ATATGGGTTT TCCTATTGAC
 3541 GTAGATAGCC CCTCCCAATG GGCGGTCCCA TATACCATAT ATGGGGCTTC CTAATACCGC
 3601 CCATAGCCAC TCCCCCATTG ACGTCAATGG TCTCTATATA TGGTCTTTCC TATTGACGTC
 3661 ATATGGGCGG TCCTATTGAC GTATATGGCG CCTCCCCCAT TGACGTCAAT TACGGTAAAT
 3721 GGCCCGCCTG GCTCAATGCC CATTGACGTC AATAGGACCA CCCACCATTG ACGTCAATGG
 3781 GATGGCTCAT TGCCCATTCA TATCCGTTCT CACGCCCCCT ATTGACGTCA ATGACGGTAA
 3841 ATGGCCCACT TGGCAGTACA TCAATATCTA TTAATAGTAA CTTGGCAAGT ACATTACTAT
 3901 TGGAAGGACG CCAGGGTACA TTGGCAGTAC TCCCATTGAC GTCAATGGCG GTAAATGGCC
 3961 CGCGATGGCT GCCAAGTACA TCCCCATTGA CGTCAATGGG GAGGGGCAAT GACGCAAATG
 4021 GGCGTTCCAT TGACGTAAAT GGGCGGTAGG CGTGCCTAAT GGGAGGTCTA TATAAGCAAT
 4081 GCTCGTTTAG GGAAC
//

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载体应用举例

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