| 质粒名称: | amilCP chromoprotein |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 117847 |
| 存储实验室: | Anthony Forster |
|
载体骨架基本信息 |
|
| 载体名称: | BioBrick pSB1C3 vector |
| 载体出品公司: | iGEM Registry |
| 载体修饰: | BioBrick sites removed |
| 载体类型: | 合成生物学；大肠杆菌 |
| 载体抗性: | 氯霉素，25μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | promoter, RBS, amilCP |
| 物种来源: | Synthetic |
|
文献引用方法 |
|
| 材料和方法部分: | amilCP chromoprotein was a gift from Anthony Forster (Addgene plasmid # 117847 ; http://n2t.net/addgene:117847 ; RRID:Addgene_117847) |
| 参考文献部分: | Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2018. 100 [pii] PubMed 29760772 |
买家导航
amilCP chromoprotein载体质粒基本信息
amilCP chromoprotein质粒图谱载体图谱和amilCP chromoprotein载体序列质粒序列多克隆位点信息
amilCP chromoprotein质粒载体简介
amilCP chromoprotein质粒序列载体序列
LOCUS amilCP chromoprotein 2800 bp DNA circular SYN 15-DEC-2025
DEFINITION BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP
chromoprotein in E. coli.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2800)
AUTHORS Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen
A, Lundin ER, Wistrand-Yuen E, Forster AC
TITLE Engineering a palette of eukaryotic chromoproteins for bacterial
synthetic biology.
JOURNAL J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0.
eCollection 2018.
PUBMED 29760772
REFERENCE 2 (bases 1 to 2800)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: "
10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT Sequence Label: amilCP chromoprotein
FEATURES Location/Qualifiers
source 1..2800
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 3..24
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
promoter 25..59
/label=BBa_J23110
/note="Anderson constitutive promoter
(https://parts.igem.org/Promoters/Catalog/Anderson).
Measured strength relative to BBa_J223100: 0.33"
RBS 68..79
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
misc_feature 755..775
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 776..833
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
primer_bind complement(857..874)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1028..1616)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1108..1127)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator complement(1799..1893)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(1914..2573)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 2488..2507
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
primer_bind 2735..2753
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
terminator complement(2757..2800)
/label=bacterial terminator
/note="putative bacterial transcription terminator"
ORIGIN
1 tggaattcgc ggccgcttct agagtttacg gctagctcag tcctaggtac aatgctagct
61 actagagaaa gaggagaaat actagatgag tgtgatcgct aaacaaatga cctacaaggt
121 ttatatgtca ggcacggtca atggacacta ctttgaggtc gaaggcgatg gaaaaggtaa
181 gccctacgag ggggagcaga cggtaaagct cactgtcacc aagggcggac ctctgccatt
241 tgcttgggat attttatcac cacagtgtca gtacggaagc ataccattca ccaagtaccc
301 tgaagacatc cctgactatg taaagcagtc attcccggag ggctatacat gggagaggat
361 catgaacttt gaagatggtg cagtgtgtac tgtcagcaat gattccagca tccaaggcaa
421 ctgtttcatc taccatgtca agttctctgg tttgaacttt cctcccaatg gacctgtcat
481 gcagaagaag acacagggct gggaacccaa cactgagcgt ctctttgcac gagatggaat
541 gctgctagga aacaacttta tggctctgaa gttagaagga ggcggtcact atttgtgtga
601 atttaaaact acttacaagg caaagaagcc tgtgaagatg ccagggtatc actatgttga
661 ccgcaaactg gatgtaacca atcacaacaa ggattacact tcggttgagc agtgtgaaat
721 ttccattgca cgcaaacctg tggtcgccta ataatactag tagcggccgc tgcagtccgg
781 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
841 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
1021 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
1081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
1141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
1201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
1261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
1321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
1381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
1441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
1501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
1561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
1621 atcctttggt cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
1681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
1741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
1801 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
1861 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
1921 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
1981 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
2041 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
2101 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
2161 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
2221 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
2281 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
2341 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
2401 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
2461 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
2521 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
2581 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
2641 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
2701 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
2761 cagataaaaa aaatccttag ctttcgctaa ggatgatttc
//
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