pRetroX-TRE3G-Luc

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pRetroX-TRE3G-Luc
质粒类型: 逆病毒载体;四环素调控载体;荧光素酶报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: TRE3GV
载体大小: 8233 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签:
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: DH5α
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 逆病毒载体pRetroX-TRE3G-Luc是pRetroX-TRE3G的阳性参照载体,受四环素诱导。
产品目录号: 631192
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pRetroX-TRE3G-Luc载体图谱



pRetroX-TRE3G-Luc 载体特征

载体简介

载体序列

LOCUS       pRetroX-TRE3G-Luc Control   8233 bp    DNA   circular            25-MAY-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT	    631192, 631194, 631188, 631190
FEATURES             Location/Qualifiers
     LTR             1..728
                     /label=5'\LTR(CMV/MSV)
     misc_feature    759..1568
                     /label=Packaging\Signal
     CDS             1967..3619
                     /label=Luciferace
     promoter        3635..4138
                     /label=pPGK
     CDS             4159..4758
                     /label=Puro(R)
     LTR             4939..5364
                     /label=3'\LTR
     polyA_signal    5266..5271
                     /label=SV40\polyA
     promoter        5644..5911
                     /label=pSV40
     rep_origin      5865..5930
                     /label=SV40\ori
     rep_origin      6221..6864
                     /label=pUC\ori
     misc_feature    complement(7011..7871)
                     /label=Amp(R)
     misc_feature    1611..1957
                     /label=pTRE3G
BASE COUNT     1958 a      2210 c      2093 g      1972 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc 
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 
      481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact 
      541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct 
      601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 
      661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg 
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt 
      781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact 
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc 
      901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt 
      961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac 
     1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc 
     1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt 
     1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag 
     1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct 
     1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc 
     1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag 
     1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca 
     1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc 
     1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc 
     1561 taggcgccgg aattgaagat ctcgatgagg ccctttcgtc ttcactcgag tttactccct 
     1621 atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag 
     1681 actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga 
     1741 gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct 
     1801 atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga acgtatgtcg 
     1861 aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc 
     1921 gcctggagca attccacaac acttttgtct tatacttgga tccaccatgg aagacgccaa 
     1981 aaacataaag aaaggcccgg cgccattcta tccgctagag gatggaaccg ctggagagca 
     2041 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc 
     2101 acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc ggttggcaga 
     2161 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc 
     2221 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc 
     2281 gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc agcctaccgt 
     2341 agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat 
     2401 aatccagaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta 
     2461 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg taccagagtc 
     2521 ctttgatcgt gacaaaacaa ttgcactgat aatgaactcc tctggatcta ctgggttacc 
     2581 taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc 
     2641 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca 
     2701 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat 
     2761 gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa ttcaaagtgc 
     2821 gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg acaaatacga 
     2881 tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag aagtcgggga 
     2941 agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc tcactgagac 
     3001 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt 
     3061 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa 
     3121 tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg taaacaatcc 
     3181 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta 
     3241 ctgggacgaa gacgaacact tcttcatagt tgaccgcttg aagtctttaa ttaaatacaa 
     3301 aggataccag gtggcccccg ctgaattgga gtcgatattg ttacaacacc ccaacatctt 
     3361 cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt 
     3421 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca 
     3481 agtaacaacc gccaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg 
     3541 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg 
     3601 cggaaagtcc aaattgtaaa atacgcgtga attctaccgg gtaggggagg cgcttttccc 
     3661 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 
     3721 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 
     3781 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 
     3841 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 
     3901 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 
     3961 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 
     4021 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 
     4081 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 
     4141 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 
     4201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 
     4261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 
     4321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 
     4381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 
     4441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 
     4501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 
     4561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 
     4621 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 
     4681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 
     4741 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca 
     4801 cgaccccatg catcggcgtc tcgagatatc agtggtccag gctctagttt tgactcaaca 
     4861 atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag 
     4921 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt 
     4981 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag 
     5041 gtcaggaaca gatggaacag ggtcgaccct agagaaccat cagatgtttc cagggtgccc 
     5101 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt 
     5161 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg 
     5221 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc 
     5281 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact 
     5341 acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag 
     5401 ggaccaccga cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg 
     5461 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 
     5521 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 
     5581 catgacccag tcacgtagcg atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt 
     5641 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     5701 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     5761 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 
     5821 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     5881 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 
     5941 aggcctaggc ttttgcaaaa agcttactgg cttaactatg cggcatcaga gcagattgta 
     6001 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 
     6061 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     6121 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     6181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     6241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     6301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     6361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     6421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     6481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     6541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     6601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     6661 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     6721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     6781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     6841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     6901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     6961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     7021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     7081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     7141 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     7201 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     7261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     7321 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     7381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     7441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     7501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     7561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     7621 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     7681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     7741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     7801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     7861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     7921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     7981 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 
     8041 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tagcttggcc attgcatacg 
     8101 ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt accgccatgt 
     8161 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 
     8221 ccatatatgg agt 
//

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