pBABE-Puro SV40LT

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Cell Biolabs, Inc.
载体名称: pBABE-Puro SV40LT, pBABEpuro SV40LT, pBABE Puro SV40 Large T
质粒类型: 哺乳动物细胞逆病毒基因表达载体
插入基因: SV40 LT, SV40gp6
插入基因Genbank ID: AF316141
插入位点: BamH I (5’端)/ BamH I (3'端)
高拷贝/低拷贝: 低拷贝
启动子: PGK
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7302 bp
5' 测序引物及序列: pBABE 5‘: CTTTATCCAGCCCTCAC
3' 测序引物及序列: pBABE 3’: ACCCTAACTGACACACATTCC
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素(Puromycin)
备注: 含有嘌呤霉素筛选基因,可以用来筛选感染稳定的细胞系。
产品目录号: --
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

pBABE-Puro SV40LT载体图谱

载体简介

载体序列

LOCUS       pBABE-puro-SV40LT         7302 bp    DNA     circular     21-SEP-2014
DEFINITION  pBABE-puro.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|709679084|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBABE-puro-SV40LT	5169|
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    12..479
                     /vntifkey="21"
                     /label=5_LTR2
     misc_feature    18..479
                     /vntifkey="21"
                     /label=3MoMuLV_LTR
     misc_feature    549..1350
                     /vntifkey="21"
                     /label=psi_plus_pack
     misc_feature    1318..1334
                     /vntifkey="21"
                     /label=pBABE_5_primer
     misc_feature    complement(3541..3561)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3547..3762)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3559..3827
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3726..3803
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3788..3807
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             complement(3861..4535)
                     /vntifkey="4"
                     /label=ORF\frame\2
     gene            3885..4484
                     /gene="puro (variant)"
                     /vntifkey="60"
     CDS             3885..4484
                     /vntifkey="4"
                     /label=ORF\frame\3
     misc_feature    4583..5176
                     /vntifkey="21"
                     /label=3MoMuLV_LTR
     misc_feature    4625..5176
                     /vntifkey="21"
                     /label=5_LTR2
     gene            complement(6314..7174)
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             complement(6314..7174)
                     /vntifkey="4"
                     /label=ORF\frame\3
     promoter        complement(7216..7244)
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    1361..3487
                     /vntifkey="21"
                     /label=SV40LT
                     /note="SV40 Large T antigen"
BASE COUNT     1862 a      1815 c      1819 g      1806 t 
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc atggataaag ttttaaacag 
     1381 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct gggggaatat 
     1441 tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg ataaaggagg 
     1501 agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag atggagtaaa 
     1561 atatgctcat caacctgact ttggaggctt ctgggatgca actgagattc caacctatgg 
     1621 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga 
     1681 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa 
     1741 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag 
     1801 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa 
     1861 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca 
     1921 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc 
     1981 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa 
     2041 taaggaatat ttgatgtata gtgccttgac tagagatcca ttttctgtta ttgaggaaag 
     2101 tttgccaggt gggttaaagg agcatgattt taatccagaa gaagcagagg aaactaaaca 
     2161 agtgtcctgg aagcttgtaa cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt 
     2221 attgcttggg atgtacttgg aatttcagta cagttttgaa atgtgtttaa aatgtattaa 
     2281 aaaagaacag cccagccact ataagtacca tgaaaagcat tatgcaaatg ctgctatatt 
     2341 tgctgacagc aaaaaccaaa aaaccatatg ccaacaggct gttgatactg ttttagctaa 
     2401 aaagcgggtt gatagcctac aattaactag agaacaaatg ttaacaaaca gatttaatga 
     2461 tcttttggat aggatggata taatgtttgg ttctacaggc tctgctgaca tagaagaatg 
     2521 gatggctgga gttgcttggc tacactgttt gttgcccaaa atggattcag tggtgtatga 
     2581 ctttttaaaa tgcatggtgt acaacattcc taaaaaaaga tactggctgt ttaaaggacc 
     2641 aattgatagt ggtaaaacta cattagcagc tgctttgctt gaattatgtg gggggaaagc 
     2701 tttaaatgtt aatttgccct tggacaggct gaactttgag ctaggagtag ctattgacca 
     2761 gtttttagta gtttttgagg atgtaaaggg cactggaggg gagtccagag atttgccttc 
     2821 aggtcaggga attaataacc tggacaattt aagggattat ttggatggca gtgttaaggt 
     2881 aaacttagaa aagaaacacc taaataaaag aactcaaata tttccccctg gaatagtcac 
     2941 catgaatgag tacagtgtgc ctaaaacact gcaggccaga tttgtaaaac aaatagattt 
     3001 taggcccaaa gattatttaa agcattgcct ggaacgcagt gagtttttgt tagaaaagag 
     3061 aataattcaa agtggcattg ctttgcttct tatgttaatt tggtacagac ctgtggctga 
     3121 gtttgctcaa agtattcaga gcagaattgt ggagtggaaa gagagattgg acaaagagtt 
     3181 tagtttgtca gtgtatcaaa aaatgaagtt taatgtggct atgggaattg gagttttaga 
     3241 ttggctaaga aacagtgatg atgatgatga agacagccag gaaaatgctg ataaaaatga 
     3301 agatggtggg gagaagaaca tggaagactc agggcatgaa acaggcattg attcacagtc 
     3361 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata 
     3421 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 
     3481 aacataagga tcccagtgtg gtggtacgta ggaattcgcc agcacagtgg tcgaccctgt 
     3541 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     3601 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 
     3661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 
     3721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     3781 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     3841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc 
     3901 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 
     3961 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 
     4021 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 
     4081 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 
     4141 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 
     4201 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 
     4261 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 
     4321 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 
     4381 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gagtgcccga 
     4441 aggaccgcgc gacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc 
     4501 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gataaaataa aagattttat 
     4561 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt 
     4621 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat 
     4681 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca 
     4741 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga 
     4801 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc 
     4861 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc 
     4921 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc 
     4981 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc 
     5041 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat 
     5101 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca 
     5161 gcgggggtct ttcatttccg acttgtggtc tcgctgcctt gggagggtct cctctgagtg 
     5221 attgactacc cgtcagcggg ggtcttcaca tgcagcatgt atcaaaatta atttggtttt 
     5281 ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg 
     5341 ggagaggcgg tttgcgtatt ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     5401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     5461 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     5521 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     5581 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     5641 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     5701 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     5761 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     5821 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     5881 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     5941 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     6001 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     6061 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     6121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     6181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     6241 ccttttaaat taaaaatgaa gtttgcggcc gcaaatcaat ctaaagtata tatgagtaaa 
     6301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     6361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     6421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     6481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     6541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     6601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     6661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     6721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     6781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     6841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     6901 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa 
     6961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     7021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     7081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     7141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     7201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     7261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac 
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng