pLJM1-EGFP

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

载体名称: pLJM1-EGFP 
出品公司: Addgene
目录编号: 19319
存储实验室: David Sabatini

载体骨架基本信息
载体名称: pLJM1
载体抗性: 氨苄霉素
载体类型: 哺乳细胞表达
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
克隆方法: 限制性内切酶
5' 克隆位点: 未知(未破坏)
3' 克隆位点: 未知 (未破坏)
5' 测序引物 CMV-F
3' 测序引物  

文献引用方法
材料和方法部分: pLJM1-EGFP was a gift from David Sabatini (Addgene plasmid # 19319)
参考文献部分: The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L, Sabatini DM. Science. 2008 Jun 13. 320(5882):1496-501. 10.1126/science.1157535 PubMed 18497260

载体质粒图谱和多克隆位点信息

pLJM1-EGFP载体图谱

载体简介

载体序列

LOCUS       pLJM1-EGFP	8083 bp 	DNA	SYN
DEFINITION  pLJM1-EGFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="pLJM1-EGFP"
                     /mol_type="other DNA"
     misc_feature    285..305
                     /label="CMV_fwd_primer"
     promoter        286..355
                     /label="CMV_promoter"
     CDS             379..1215
                     /label="ORF frame 1"
     gene            382..1095
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(424..445)
                     /label="EGFP_N_primer"
     misc_feature    562..591
                     /label="Y66 (EGFP)"
     misc_feature    1032..1053
                     /label="EGFP_C_primer"
     misc_feature    1170..1185
                     /label="cPPT"
     CDS             complement(1251..1865)
                     /label="ORF frame 3"
     CDS             1624..2463
                     /label="ORF frame 1"
     CDS             complement(1669..2514)
                     /label="ORF frame 2"
     gene            1864..2463
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    2574..2589
                     /label="cPPT"
     misc_feature    2574..2595
                     /label="U3PPT"
     misc_feature    2591..2643
                     /label="delta_U3"
     misc_feature    2644..2824
                     /label="HIV-1_5_LTR"
     misc_feature    2644..2824
                     /label="truncHIV-1_3_LTR"
     terminator      2899..3018
                     /label="SV40_PA_terminator"
     misc_feature    2987..3006
                     /label="EBV_rev_primer"
     rep_origin      3036..3134
                     /label="SV40_origin"
     misc_feature    3119..3138
                     /label="SV40pro_F_primer"
     promoter        complement(3213..3231)
                     /label="T7_promoter"
     promoter        complement(3241..3257)
                     /label="M13_forward20_primer"
     misc_feature    complement(3250..3272)
                     /label="M13_pUC_fwd_primer"
     misc_feature    3250..3398
                     /label="lacZ_a"
     rep_origin      3416..3722
                     /label="f1_origin"
     promoter        3915..3943
                     /label="AmpR_promoter"
     gene            3985..4845
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             3985..4845
                     /label="ORF frame 1"
     rep_origin      5000..5619
                     /label="pBR322_origin"
     promoter        5928..5957
                     /label="lac_promoter"
     misc_feature    5971..5993
                     /label="M13_pUC_rev_primer"
     promoter        5992..6010
                     /label="M13_reverse_primer"
     promoter        6027..6046
                     /label="T3_promoter"
     misc_feature    6300..6480
                     /label="HIV-1_5_LTR"
     misc_feature    6300..6480
                     /label="truncHIV-1_3_LTR"
     misc_feature    6591..6635
                     /label="HIV-1_psi_pack"
     CDS             7023..7880
                     /label="ORF frame 3"
     misc_feature    7145..7378
                     /label="RRE"
ORIGIN
    1 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC
   61 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC
  121 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC
  181 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA
  241 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG
  301 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA
  361 GCGCTACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC
  421 ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC
  481 GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG
  541 CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC
  601 TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC
  661 CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG
  721 TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC
  781 GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG
  841 GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC
  901 GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
  961 CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG
 1021 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG
 1081 GACGAGCTGT ACAAGTCCGG ACTCAGATCT CGAGCTCAAG CTTCGAATTC TCGACCTCGA
 1141 GACAAATGGC AGTATTCATC CACAATTTTA AAAGAAAAGG GGGGATTGGG GGGTACAGTG
 1201 CAGGGGAAAG AATAGTAGAC ATAATAGCAA CAGACATACA AACTAAAGAA TTACAAAAAC
 1261 AAATTACAAA AATTCAAAAT TTTCGGGTTT ATTACAGGGA CAGCAGAGAT CCACTTTGGC
 1321 CGCGGCTCGA GGGGGTTGGG GTTGCGCCTT TTCCAAGGCA GCCCTGGGTT TGCGCAGGGA
 1381 CGCGGCTGCT CTGGGCGTGG TTCCGGGAAA CGCAGCGGCG CCGACCCTGG GTCTCGCACA
 1441 TTCTTCACGT CCGTTCGCAG CGTCACCCGG ATCTTCGCCG CTACCCTTGT GGGCCCCCCG
 1501 GCGACGCTTC CTGCTCCGCC CCTAAGTCGG GAAGGTTCCT TGCGGTTCGC GGCGTGCCGG
 1561 ACGTGACAAA CGGAAGCCGC ACGTCTCACT AGTACCCTCG CAGACGGACA GCGCCAGGGA
 1621 GCAATGGCAG CGCGCCGACC GCGATGGGCT GTGGCCAATA GCGGCTGCTC AGCAGGGCGC
 1681 GCCGAGAGCA GCGGCCGGGA AGGGGCGGTG CGGGAGGCGG GGTGTGGGGC GGTAGTGTGG
 1741 GCCCTGTTCC TGCCCGCGCG GTGTTCCGCA TTCTGCAAGC CTCCGGAGCG CACGTCGGCA
 1801 GTCGGCTCCC TCGTTGACCG AATCACCGAC CTCTCTCCCC AGGGGGATCC ACCGGAGCTT
 1861 ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC
 1921 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG
 1981 GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC
 2041 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG
 2101 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC
 2161 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG
 2221 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG
 2281 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC
 2341 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC
 2401 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC
 2461 TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG
 2521 GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA
 2581 AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATCTGCT TTTTGCTTGT
 2641 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC
 2701 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG
 2761 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT
 2821 AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA
 2881 TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
 2941 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
 3001 CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCATCC
 3061 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA
 3121 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT
 3181 TTTTTGGAGG CCTAGGGACG TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC
 3241 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG
 3301 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG
 3361 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT
 3421 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC
 3481 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
 3541 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
 3601 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT
 3661 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC
 3721 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC
 3781 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
 3841 AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA
 3901 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT
 3961 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC
 4021 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA
 4081 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT
 4141 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT
 4201 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC
 4261 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG
 4321 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG
 4381 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC
 4441 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA
 4501 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA
 4561 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT
 4621 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA
 4681 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG
 4741 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT
 4801 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT
 4861 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG
 4921 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA
 4981 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA
 5041 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA
 5101 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT
 5161 GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA
 5221 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
 5281 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG
 5341 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
 5401 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA
 5461 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT
 5521 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG
 5581 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
 5641 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
 5701 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
 5761 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
 5821 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
 5881 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
 5941 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
 6001 TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG
 6061 CTGCAAGCTT AATGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA
 6121 GTTAGCAACA TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG GTGGAAGTAA
 6181 GGTGGTACGA TCGTGCCTTA TTAGGAAGGC AACAGACGGG TCTGACATGG ATTGGACGAA
 6241 CCACTGAATT GCCGCATTGC AGAGATATTG TATTTAAGTG CCTAGCTCGA TACATAAACG
 6301 GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC
 6361 TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT
 6421 GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA
 6481 GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG
 6541 GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC
 6601 AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA
 6661 GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA
 6721 ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC
 6781 TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT
 6841 TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT
 6901 GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA
 6961 AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT CTTCAGACCT GGAGGAGGAG
 7021 ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT
 7081 TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG
 7141 GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT
 7201 CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA
 7261 ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA
 7321 AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG
 7381 GGATTTGGGG TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT
 7441 GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG
 7501 AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG
 7561 AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA
 7621 ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG
 7681 GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC
 7741 CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG
 7801 AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC
 7861 GGTATCGATC ACGAGACTAG CCTCGAGCGG CCGCCCCCTT CACCGAGGGC CTATTTCCCA
 7921 TGATTCCTTC ATATTTGCAT ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT
 7981 TGACTGTAAA CACAAAGATA TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG
 8041 GGTAGTTTGC AGTTTTAAAA TTATGTTTTA AAATGGACTA TCA
//

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