pLenti CMV/TO Puro empty (w175-1)

价格:1200元

联系方式:I47-825O-882O

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载体基本信息

质粒名称: pLenti CMV/TO Puro empty (w175-1) 
出品公司: Addgene
目录编号: 17482
存储实验室: Eric Campeau

载体骨架基本信息
载体名称: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
载体抗性: 氨苄霉素
空载体大小: 8007 bp
载体类型: 哺乳细胞表达
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
克隆方法: 限制性内切酶
5' 克隆位点: Bam HI(未破坏)
3' 克隆位点: Xba I(未破坏)
5' 测序引物 CMV-F
3' 测序引物  

文献引用方法
材料和方法部分: pLenti CMV/TO Puro empty (w175-1) was a gift from Eric Campeau (Addgene plasmid # 17482)
参考文献部分: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394

载体质粒图谱和多克隆位点信息

pLenti CMV/TO Puro empty (w175-1)载体图谱

载体简介

载体序列

LOCUS       pLenti CMV/TO Puro empty (w175-1)	8007 bp 	DNA	SYN
DEFINITION  pLenti CMV/TO Puro empty (w175-1)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="pLenti CMV/TO Puro empty (w175-1)"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             132..992
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     CDS             3168..3668
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /label="RRE"
     CDS             3629..4171
                     /label="ORF frame 2"
     misc_feature    4016..4031
                     /label="cPPT"
     promoter        4149..4653
                     /label="CMV_immearly_promoter"
     misc_feature    4156..4443
                     /label="CAG_enhancer"
     misc_feature    4610..4630
                     /label="CMV_fwd_primer"
     misc_feature    4661..4700
                     /label="tetO"
     misc_feature    4741..4765
                     /label="attB1"
     misc_feature    4834..4858
                     /label="attB2"
     misc_feature    4891..5466
                     /label="WPRE"
     CDS             4979..5608
                     /label="ORF frame 2"
     misc_feature    complement(5526..5549)
                     /label="MSCV_rev_primer"
                     /label="ORF frame 2"
     misc_feature    5923..5942
                     /label="mPGK_F_primer"
     CDS             complement(5936..6661)
                     /label="ORF frame 3"
     gene            6011..6610
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    6731..6746
                     /label="cPPT"
     misc_feature    6731..6752
                     /label="U3PPT"
     misc_feature    6801..6981
                     /label="HIV-1_5_LTR"
     misc_feature    6801..6981
                     /label="truncHIV-1_3_LTR"
     terminator      7056..7175
                     /label="SV40_PA_terminator"
     misc_feature    7144..7163
                     /label="EBV_rev_primer"
     rep_origin      7193..7270
                     /label="SV40_origin"
     misc_feature    7255..7274
                     /label="SV40pro_F_primer"
     promoter        complement(7361..7379)
                     /label="T7_promoter"
     promoter        complement(7389..7405)
                     /label="M13_forward20_primer"
     misc_feature    complement(7398..7420)
                     /label="M13_pUC_fwd_primer"
     misc_feature    7398..7541
                     /label="lacZ_a"
     rep_origin      7567..7873
                     /label="f1_origin"
ORIGIN
    1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
   61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
  121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
  181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
  241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
  301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
  361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
  421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
  481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
  541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
  601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
  661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
  721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
  781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
  841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
  901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
  961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
 4321 tatCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
 4381 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
 4441 ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT
 4501 GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC
 4561 CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC
 4621 GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCCCTATCAG TGATAGAGAT
 4681 CTCCCTATCA GTGATAGAGA TCGTCGACTA GTCCAGTGTG GTGGAATTCT GCAGATATCA
 4741 ACAAGTTTGT ACAAAAAAGC AGGCTCCACC ATGGGAACCA ATTCAGTCGA CTGGATCCGG
 4801 TACCGAATTC GCGGCCGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG
 4861 ATATCCAGCA CAGTGGCGGC CGCTCGAcaa tcaacctctg gattacaaaa tttgtgaaag
 4921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
 4981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
 5041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
 5101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
 5161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
 5221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
 5281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
 5341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
 5401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
 5461 ttgggccgcc tccccgcctg GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT
 5521 CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC
 5581 CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGGC TCCGTTCTTT GGTGGCCCCT
 5641 TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCGCCCCG CAGCTCGCGT
 5701 CGTGCAGGAC GTGACAAATG GAAGTAGCAC GTCTCACTAG TCTCGTGCAG ATGGACAGCA
 5761 CCGCTGAGCA ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG CCAATAGCAG CTTTGCTCCT
 5821 TCGCTTTCTG GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG GGGCGGGCTC AGGGGCGGGC
 5881 TCAGGGGCGG GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC GGCATTCTGC ACGCTTCAAA
 5941 AGCGCACGTC TGCCGCGCTG TTCTCCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCAGCC
 6001 CAAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC
 6061 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT
 6121 CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT
 6181 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC
 6241 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA
 6301 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG
 6361 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA
 6421 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC
 6481 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC
 6541 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC
 6601 CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG CACGACCCCA
 6661 TGCATCTCGA GGGCCCGGTA Cctttaagac caatgactta caaggcagct gtagatctta
 6721 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
 6781 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
 6841 ctggctgcct agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
 6901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
 6961 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
 7021 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
 7081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
 7141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
 7201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
 7261 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
 7321 tggaggccta ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac
 7381 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
 7441 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
 7501 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt
 7561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
 7621 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
 7681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
 7741 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
 7801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
 7861 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
 7921 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
 7981 aacaaaatat taacgtttac aatttcc
//

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