pLVX-DsRed-Monomer-C1

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pLVX-DsRed-Monomer-C1
质粒类型: 慢病毒表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8753 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: DsRed-Monomer(N-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系,293、CV-1、CHO等
备注: pLVX-DsRed-Monomer-C1载体表达N端DsRed-Monomer融合蛋白;
DsRed-Monomer是DsRed的单体突变体,与DsRed相比,编码序列经过了人工优化,适用于在哺乳动物细胞中的高水平表达;
CMV启动子是过表达启动子。
产品目录号: 632153
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-DsRed-Monomer-C1载体图谱



pLVX-DsRed-Monomer-C1多克隆位点

pLVX-DsRed-Monomer-C1载体特征

载体简介

Description
pLVX-DsRed-Monomer-C1 is an HIV-1-based, lentiviral expression vector that allows you to express your gene of interest fused to DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein. Genes cloned into the multiple cloning site (MCS), located at the C-terminal end of the DsRed-Monomer coding sequence, are expressed as C-terminal fusions of the DsRed-Monomer protein. Expression of the fusion protein is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE) located just upstream of the DsRed-Monomer coding sequence. Lentiviral particles derived from the vector allow the expression of DsRed-Monomer fusion proteins in virtually any cell type, including primary cells. The unmodified vector expresses DsRed-Monomer, and may be used to produce marker virus to optimize infection protocols.

pLVX-DsRed-Monomer-C1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (1), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-DsRed-Monomer-C1 also contains a central polypurine tract (cPPT) element that increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more efficient transduction (3).

In addition to lentiviral elements, pLVX-DsRed-Monomer-C1 contains a puromycin resistance gene (Puro) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Amp) for propagation and selection in bacteria.

Use
pLVX-DsRed-Monomer-C1 constitutively expresses your gene of interest from PCMV IE when transduced into target cells. Before the vector can be transduced into cells, however, it must be transfected into 293T packaging cells with our Lenti-X™ HTX Packaging System (Cat. Nos. 631247 and 631249). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).

载体序列

LOCUS       pLVX-DsRed-Monomer-C1   8753 bp    DNA   circular             9-NOV-2009
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     Cat. No. 632153
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4721..5312
                     /label=WPRE
     rep_origin      complement(6621..7294)
                     /label=pUC\origin
     CDS             complement(7439..8435)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5515..6151
                     /label=3'\LTR
     CDS             2815..3570
                     /label=DsRed-Monomer
     misc_feature    3564..3566
                     /label=Stop
     misc_feature    3568..3570
                     /label=Stop
     misc_feature    3572..3574
                     /label=Stop
     misc_feature    3503..3556
                     /label=MCS
     promoter        3579..4087
                     /label=PGK\Promoter
     CDS             4108..4707
                     /label=Puro(R)
BASE COUNT     2170 a      2278 c      2314 g      1991 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggtcgc caccatggac 
     2821 aacaccgagg acgtcatcaa ggagttcatg cagttcaagg tgcgcatgga gggctccgtg 
     2881 aacggccact acttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag 
     2941 accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc 
     3001 ccccagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac 
     3061 atgaagctgt ccttccccga gggcttcacc tgggagcgct ccatgaactt cgaggacggc 
     3121 ggcgtggtgg aggtgcagca ggactcctcc ctgcaggacg gcaccttcat ctacaaggtg 
     3181 aagttcaagg gcgtgaactt ccccgccgac ggccccgtaa tgcagaagaa gactgccggc 
     3241 tgggagccct ccaccgagaa gctgtacccc caggacggcg tgctgaaggg cgagatctcc 
     3301 cacgccctga agctgaagga cggcggccac tacacctgcg acttcaagac cgtgtacaag 
     3361 gccaagaagc ccgtgcagct gcccggcaac cactacgtgg actccaagct ggacatcacc 
     3421 aaccacaacg aggactacac cgtggtggag cagtacgagc acgccgaggc ccgccactcc 
     3481 ggctcccagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc 
     3541 gcgggcccgg gatccaccgg atctagataa ctgatcataa ttctaccggg taggggaggc 
     3601 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 
     3661 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 
     3721 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 
     3781 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 
     3841 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 
     3901 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 
     3961 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 
     4021 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 
     4081 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc 
     4141 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 
     4201 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 
     4261 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 
     4321 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 
     4381 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 
     4441 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 
     4501 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 
     4561 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 
     4621 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 
     4681 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa 
     4741 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     4801 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     4861 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     4921 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     4981 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 
     5041 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     5101 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 
     5161 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     5221 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     5281 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa 
     5341 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta 
     5401 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca 
     5461 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa 
     5521 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 
     5581 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc 
     5641 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa 
     5701 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 
     5761 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg 
     5821 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg 
     5881 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc 
     5941 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg 
     6001 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 
     6061 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 
     6121 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 
     6181 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt 
     6241 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     6301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     6361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     6421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     6481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     6541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     6601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     6661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     6721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     6781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     6841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     6901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     6961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     7021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     7081 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
     7141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     7201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     7261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     7321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     7381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     7441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     7501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     7561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     7621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     7681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     7741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     7801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     7861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     7921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     7981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     8041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     8101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     8161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     8221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     8281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     8341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     8401 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 
     8461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     8521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     8581 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 
     8641 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 
     8701 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt 
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng