| 质粒名称: | FUW-tetO-lox-hKLF4 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20727 |
| 存储实验室: | Rudolf Jaenisch |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | Carlos Lois |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6454 bp |
| 完整质粒大小: | 7976 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | KLF4 |
| 物种来源: | 人源 |
| 插入大小: | 1522 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | LNCX |
|
文献引用方法 |
|
| 材料和方法部分: | FUW-tetO-lox-hKLF4 was a gift from Rudolf Jaenisch (爱迪基因 plasmid # 20727) |
| 参考文献部分: | Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors. Frank Soldner, Dirk Hockemeyer, Caroline Beard, Qing Gao, George W. Bell, Elizabeth G. Cook, Gunnar Hargus, Alexandra Blak, Oliver Cooper, Maisam Mitalipova, Ole Isacson, Rudolf Jaenisch. Cell, Volume 136, Issue 5, 964-977, 6 March 2009 10.1016/j.cell.2009.02.013 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUW-tetO-lox-hKLF4 7976 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FUW-tetO-lox-hKLF4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7976)
AUTHORS Frank Soldner, Dirk Hockemeyer, Caroline Beard, Qing Gao, George W.
Bell, Elizabeth G. Cook, Gunnar Hargus, Alexandra Blak, Oliver
Cooper, Maisam Mitalipova, Ole Isacson, Rudolf Jaenisch
TITLE Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells
Free of Viral Reprogramming Factors
JOURNAL Cell, Volume 136, Issue 5, 964-977, 6 March 2009
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|FUW-tetO-lox-hKLF4|
FEATURES Location/Qualifiers
source 1..7976
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 7..386
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 388..586
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 541..561
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 604..784
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 831..956
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1449..1682
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2209..2326
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2385..2655
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2688..2725
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2722..2746
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 4365..4953
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4418..4438)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4836..4847)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4956..4972)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4957..4973)
/label=pBluescriptKS
/note="For pBluescript vector"
protein_bind complement(5477..5510)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 5524..5704
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5730..5747)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5736..5847
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(5993..6581)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6073..6092)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6752..7612)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7375..7394
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7613..7717)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7792..7811)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
2341 ggacagcaga gatccagttt ggttaatgat ctcgagttta ccactcccta tcagtgatag
2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
2641 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgagta ggcgtgtacg
2701 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
2761 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
2821 attccttctc ttcgttgact ttggggttca ggtgccccag ctgcttcggg ctgccgagga
2881 ccttctgggc ccccacatta atgaggcagc cacctggcga gtctgacatg gctgtcagcg
2941 acgcgctgct cccatctttc tccacgttcg cgtctggccc ggcgggaagg gagaagacac
3001 tgcgtcaagc aggtgccccg aataaccgct ggcgggagga gctctcccac atgaagcgac
3061 ttcccccagt gcttcccggc cgcccctatg acctggcggc ggcgaccgtg gccacagacc
3121 tggagagcgg cggagccggt gcggcttgcg gcggtagcaa cctggcgccc ctacctcgga
3181 gagagaccga ggagttcaac gatctcctgg acctggactt tattctctcc aattcgctga
3241 cccatcctcc ggagtcagtg gccgccaccg tgtcctcgtc agcgtcagcc tcctcttcgt
3301 cgtcgccgtc gagcagcggc cctgccagcg cgccctccac ctgcagcttc acctatccga
3361 tccgggccgg gaacgacccg ggcgtggcgc cgggcggcac gggcggaggc ctcctctatg
3421 gcagggagtc cgctccccct ccgacggctc ccttcaacct ggcggacatc aacgacgtga
3481 gcccctcggg cggcttcgtg gccgagctcc tgcggccaga attggacccg gtgtacattc
3541 cgccgcagca gccgcagccg ccaggtggcg ggctgatggg caagttcgtg ctgaaggcgt
3601 cgctgagcgc ccctggcagc gagtacggca gcccgtcggt catcagcgtc agcaaaggca
3661 gccctgacgg cagccacccg gtggtggtgg cgccctacaa cggcgggccg ccgcgcacgt
3721 gccccaagat caagcaggag gcggtctctt cgtgcaccca cttgggcgct ggaccccctc
3781 tcagcaatgg ccaccggccg gctgcacacg acttccccct ggggcggcag ctccccagca
3841 ggactacccc gaccctgggt cttgaggaag tgctgagcag cagggactgt caccctgccc
3901 tgccgcttcc tcccggcttc catccccacc cggggcccaa ttacccatcc ttcctgcccg
3961 atcagatgca gccgcaagtc ccgccgctcc attaccaaga gctcatgcca cccggttcct
4021 gcatgccaga ggagcccaag ccaaagaggg gaagacgatc gtggccccgg aaaaggaccg
4081 ccacccacac ttgtgattac gcgggctgcg gcaaaaccta cacaaagagt tcccatctca
4141 aggcacacct gcgaacccac acaggtgaga aaccttacca ctgtgactgg gacggctgtg
4201 gatggaaatt cgcccgctca gatgaactga ccaggcacta ccgtaaacac acggggcacc
4261 gcccgttcca gtgccaaaaa tgcgaccgag cattttccag gtcggaccac ctcgccttac
4321 acatgaagag gcatttttaa gaattcgata tcaagcttat cgataatcaa cctctggatt
4381 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
4441 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
4501 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
4561 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
4621 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
4681 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
4741 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
4801 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
4861 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
4921 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
4981 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
5041 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
5101 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
5161 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
5221 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
5281 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
5341 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
5401 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
5461 tgcatccgga ctcgagataa cttcgtataa tgtatgctat acgaagttat tccggactgt
5521 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
5581 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
5641 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
5701 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
5761 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
5821 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
5881 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgtgag
5941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
6001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
6061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
6121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
6181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
6241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
6301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
6361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
6421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
6481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
6541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
6601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
6661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
6721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
6781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
6841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
6901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
6961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
7021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
7081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
7141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
7201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
7261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
7321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
7381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
7441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
7501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
7561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
7621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
7681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
7741 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
7801 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
7861 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
7921 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatata
//
