| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pIB/V5-His |
| 质粒类型: | 昆虫表达载体 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 启动子: | OplE2 |
| 载体大小: | 3521 bp |
| 5' 测序引物及序列: | OpIE2 Forward：CGCAACGATCTGGTAAACAC |
| 3' 测序引物及序列: | 5'd[CGCAACGATCTGGTAAACAC]3' |
| 载体标签: | V5 Epitope Tag , His Tag (C-末端) |
| 载体抗性: | 氨苄青霉素 |
| 真核筛选标记: | Blasticidin |
| 克隆菌株: | TOP10、DH5 |
| 宿主细胞（系）: | Sf9 、High Five |
| 备注: | -- |
| 产品目录号: | V802001 |
| 瞬表达/稳表达: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
The InsectSelect BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate
expression, analysis, and detection of recombinant protein in insect cells:
• The OpIE2 promoter for constitutive expression
• The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
载体序列
LOCUS pIB/V5-His 3521 bp DNA SYN
DEFINITION pIB/V5-His
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3521
/organism="pIB/V5-His"
/mol_type="other DNA"
misc_feature 511..530
/label="OpIE2_fwd_primer"
rep_origin complement(987..1606)
/label="pBR322_origin"
promoter 1980..2047
/label="EM7_promoter"
gene 2048..2446
/label="blast (variant)"
/gene="blast (variant)"
gene 2566..3426
/label="Ampicillin"
/gene="Ampicillin"
CDS 2566..3426
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG
61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT
121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC
181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC
241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC
301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT
361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA
421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC
481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG
541 CATCTGTTCG AATTTAAAGC TTGGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA
601 TTCTGCAGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGC GGTTCGAAGG
661 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTC ATCATCACCA
721 TCACCATTGA GTTTATCTGA CTAAATCTTA GTTTGTATTG TCATGTTTTA ATACAATATG
781 TTATGTTTAA ATATGTTTTT AATAAATTTT ATAAAATAAT TTCAACTTTT ATTGTAACAA
841 CATTGTCCAT TTACACACTC CTTTCAAGCG CGTGGGATCG ATGCTCACTC AAAGGCGGTA
901 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
961 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
1021 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
1081 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
1141 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
1201 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
1261 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
1321 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
1381 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
1441 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
1501 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
1561 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
1621 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCGAAA CACGCACGGC
1681 GCGCGCACGC AGCTTAGCAC AAACGCGTCG TTGCACGCGC CCACCGCTAA CCGCAGGCCA
1741 ATCGGTCGGC CGGCCTCATA TCCGCTCACC AGCCGCGTCC TATCGGGCGC GGCTTCCGCG
1801 CCCATTTTGA ATAAATAAAC GATAACGCCG TTGGTGGCGT GAGGCATGTA AAAGGTTACA
1861 TCATTATCTT GTTCGCCATC CGGTTGGTAT AAATAGACGT TCATGTTGGT TTTTGTTTCA
1921 GTTGCAAGTT GGCTGCGGCG CGCGCAGCAC CTTTGCCGGG ATCTGCCGGG CTGCAGCACG
1981 TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA
2041 CTAAACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC
2101 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG
2161 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGCGCAGA
2221 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC
2281 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTTCTTCT
2341 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT
2401 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAAGCAC TTCGTGGCCG
2461 AGGAGCAGGA CTGACACGTC CCGGGAGATC TGCATGTCTA CTAAACTCAC AAATTAGAGC
2521 TTCAATTTAA TTATATCAGT TATTACCCAT TGAAAAAGGA AGAGTATGAG TATTCAACAT
2581 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
2641 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC
2701 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA
2761 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
2821 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA
2881 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
2941 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG
3001 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
3061 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
3121 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA
3181 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
3241 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA
3301 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
3361 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
3421 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT
3481 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC T
//
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