pYES2-NTB

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pYES2-NTB, pYES2/NT B
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6039 bp
5' 测序引物及序列: T7: TAATACGACTCACTATAGGG
3' 测序引物及序列: CYC1 Terminator: GTGACATAACTAATTACATGATG
载体标签: N-His, N-Xpress, C-His, C-V5
载体抗性: 氨苄
筛选标记: URA3
备注: 利用半乳糖诱导蛋白在酿酒酵母中表达。
产品目录号: V8252-20
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYES2-NTB载体图谱

pYES2-NTB多克隆位点

载体简介

pYES2 NT B的是一个6.0 kb的载体,设计用来在酿酒酵母(Saccharomyces cerevisiae)中诱导表达重组蛋白。

该载体包含以下元素:

1. 酵母GAL1启动子,能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白,同时能够被葡萄糖抑制表达

2.多克隆位点可以使用的很多限制酶切位点,便于基因插入。

3.CYC1终止子能够有效终止mRNA的转录。

4.能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。

5.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。

6. 载体本身带有N端His和Xpress标签,同时载体C端也带有V5抗原表位和His纯化标签。

8. 在酵母中,利用载体上的2μ复制子维持质粒的高拷贝。

载体序列

LOCUS       pYES2/NT\B	6039 bp 	DNA     circular	SYN
DEFINITION  pYES2/NT B
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="pYES2/NT B"
                     /mol_type="other DNA"
     promoter        1..451
                     /label="GAL1_promoter"
     misc_feature    414..437
                     /label="GAL1_primer"
     promoter        475..493
                     /label="T7_promoter"
     misc_feature    542..560
                     /label="Xpress_fwd_primer"
     misc_feature    543..575
                     /label="T7_leader"
     misc_feature    579..602
                     /label="Xpress_EK"
     terminator      792..1037
                     /label="CYC1_terminator"
     misc_feature    complement(801..819)
                     /label="CYC1_primer"
     misc_feature    complement(801..819)
                     /label="pYESTrp_rev_primer"
     rep_origin      complement(1265..1884)
                     /label="pBR322_origin"
     CDS             complement(2039..2899)
                     /label="ORF frame 3"
     CDS             complement(2995..3798)
                     /label="ORF frame 1"
     gene            complement(2998..3798)
                     /label="URA3"
                     /gene="URA3"
     CDS             3287..3763
                     /label="ORF frame 2"
     promoter        complement(3800..4025)
                     /label="URA3_promoter"
     rep_origin      4028..5499
                     /label="2micron_origin"
ORIGIN
    1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
   61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
  121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
  181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
  241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
  301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
  361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
  421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
  481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
  541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
  601 AGGTACCTAA GGATCCAGTG TGGTGGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT
  661 CGAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
  721 TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CCTAGAGGGC
  781 CGCATCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC CTCCCCCCAC ATCCGCTCTA
  841 ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT
  901 ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC
  961 GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG
 1021 GCTTTAATTT GCAAGCTGCG GCCCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
 1081 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
 1141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
 1201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGCCCAGG AACCGTAAAA
 1261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
 1321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
 1381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
 1441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
 1501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
 1561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
 1621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
 1681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
 1741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
 1801 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
 1861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
 1921 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
 1981 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
 2041 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
 2101 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GCGCTTACCA TCTGGCCCCA
 2161 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
 2221 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
 2281 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
 2341 TTGTTGGCAT TGCTACAGGC ATCGTGGTGT CACTCTCGTC GTTTGGTATG GCTTCATTCA
 2401 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
 2461 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
 2521 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
 2581 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
 2641 CTTGCCCGGC GTCAATACGG GATAATAGTG TATCACATAG CAGAACTTTA AAAGTGCTCA
 2701 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
 2761 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
 2821 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
 2881 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATGGGT AATAACTGAT ATAATTAAAT
 2941 TGAAGCTCTA ATTTGTGAGT TTAGTATACA TGCATTTACT TATAATACAG TTTTTTAGTT
 3001 TTGCTGGCCG CATCTTCTCA AATATGCTTC CCAGCCTGCT TTTCTGTAAC GTTCACCCTC
 3061 TACCTTAGCA TCCCTTCCCT TTGCAAATAG TCCTCTTCCA ACAATAATAA TGTCAGATCC
 3121 TGTAGAGACC ACATCATCCA CGGTTCTATA CTGTTGACCC AATGCGTCTC CCTTGTCATC
 3181 TAAACCCACA CCGGGTGTCA TAATCAACCA ATCGTAACCT TCATCTCTTC CACCCATGTC
 3241 TCTTTGAGCA ATAAAGCCGA TAACAAAATC TTTGTCGCTC TTCGCAATGT CAACAGTACC
 3301 CTTAGTATAT TCTCCAGTAG ATAGGGAGCC CTTGCATGAC AATTCTGCTA ACATCAAAAG
 3361 GCCTCTAGGT TCCTTTGTTA CTTCTTCTGC CGCCTGCTTC AAACCGCTAA CAATACCTGG
 3421 GCCCACCACA CCGTGTGCAT TCGTAATGTC TGCCCATTCT GCTATTCTGT ATACACCCGC
 3481 AGAGTACTGC AATTTGACTG TATTACCAAT GTCAGCAAAT TTTCTGTCTT CGAAGAGTAA
 3541 AAAATTGTAC TTGGCGGATA ATGCCTTTAG CGGCTTAACT GTGCCCTCCA TGGAAAAATC
 3601 AGTCAAGATA TCCACATGTG TTTTTAGTAA ACAAATTTTG GGACCTAATG CTTCAACTAA
 3661 CTCCAGTAAT TCCTTGGTGG TACGAACATC CAATGAAGCA CACAAGTTTG TTTGCTTTTC
 3721 GTGCATGATA TTAAATAGCT TGGCAGCAAC AGGACTAGGA TGAGTAGCAG CACGTTCCTT
 3781 ATATGTAGCT TTCGACATGA TTTATCTTCG TTTCCTGCAG GTTTTTGTTC TGTGCAGTTG
 3841 GGTTAAGAAT ACTGGGCAAT TTCATGTTTC TTCAACACTA CATATGCGTA TATATACCAA
 3901 TCTAAGTCTG TGCTCCTTCC TTCGTTCTTC CTTCTGTTCG GAGATTACCG AATCAAAAAA
 3961 ATTTCAAAGA AACCGAAATC AAAAAAAAGA ATAAAAAAAA AATGATGAAT TGAATTGAAA
 4021 AGCTAGCTTA TCGATGATAA GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA
 4081 TACTAGATAC TCCGTCTACT GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG
 4141 GCCTTACCAC TCTTTTGTTA CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA
 4201 TTCTATCTTC GCGATGTAGT AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG
 4261 GCACTTCTAC AATGGCTGCC ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT
 4321 ATTCGAATAC GCTTTGAGGA GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC
 4381 GATCGTACTT GTTACCCATC ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA
 4441 ACAGATAGTA TATTTGAACC TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT
 4501 GTATGTATTT CGGTTCCTGG AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC
 4561 GCATCCCCGG TTCATTTTCT GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC
 4621 GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA
 4681 ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC
 4741 CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA
 4801 TTTTTCAAAC AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC
 4861 TATTTTACCA ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC
 4921 GCTATTTTTC TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG
 4981 CAGTCTCTTG ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG
 5041 TGTCTATTTT CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA
 5101 GCGAAGCTGC GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT
 5161 GTGGATTGCG CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG
 5221 AAAATTATGA ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA
 5281 TTTTCGTATT GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA
 5341 GAGTAATACT AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG
 5401 AGCGAAAGGT GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA
 5461 TACTTTTGAG CAATGTTTGT GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT
 5521 AATCAGAAAA GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT
 5581 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG
 5641 CCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT
 5701 GTTCCAGTTT CCAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA
 5761 AAAAGGGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
 5821 GGGTCGAGGT GCCGTAAAGC AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT
 5881 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG
 5941 GCTAGGGCGG TGGGAAGTGT AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT
 6001 AATGGGGCGC TACAGGGCGC GTGGGGATGA TCCACTAGT
//

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载体应用举例

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