pTrc99a

价格:800元

联系方式:I47-825O-882O

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pTrc 99a载体基本信息

出品公司: --
别名: pTrc99a, pTrc 99a
质粒类型: 大肠杆菌蛋白表达载体
拷贝水平: 高拷贝
启动子: Trc
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4176 bp
5' 测序引物及序列: RV-M: GAGCGGATAACAATTTCACACAGG
3' 测序引物及序列: pTrcHis-R: GATTTAATCTGTATCAGG
载体标签: none
载体抗性: 氨苄
备注:
宿主菌: E.coli. 相关载体: pBR322, pTrc97.
产品目录号: --
稳定性: 瞬时表达
组成型: 组成型
病毒/非病毒: 非病毒

pTrc 99a载体质粒图谱和多克隆位点信息

pTrc99a载体图谱

pTrc 99a载体简介

细菌表达,可诱导蛋白表达,使用IPTG进行诱导(1 mM IPTG)。

pTrc 99a载体序列

LOCUS       pTrc99A    4176 bp     DNA  circular    SYN
DEFINITION  pTrc99A
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="pTrc99A"
                     /mol_type="other DNA"
     promoter        193..266
                     /label="trc_promoter"
     misc_feature    235..257
                     /label="M13_pUC_rev_primer"
     misc_feature    complement(359..376)
                     /label="pTrcHis_rev_primer"
     misc_feature    complement(359..376)
                     /label="pBAD_rev_primer"
     terminator      409..566
                     /label="rrnB_terminator"
     terminator      532..575
                     /label="rrnB_T1_terminator"
     terminator      707..734
                     /label="rrnB_T2_terminator"
     promoter        776..804
                     /label="AmpR_promoter"
     gene            846..1706
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             846..1706
                     /label="ORF frame 3"
     rep_origin      1861..2480
                     /label="pBR322_origin"
     misc_feature    2877..2899
                     /label="pGEX_3_primer"
     misc_feature    3046..4137
                     /label="lacI"
     CDS             3178..4137
                     /label="ORF frame 1"
ORIGIN
    1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
   61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
  121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
  181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
  241 taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatcctcta
  301 gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc
  361 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
  421 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
  481 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
  541 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
  601 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
  661 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
  721 acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa
  781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
  841 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
  901 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
  961 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
 1021 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
 1081 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
 1141 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
 1201 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
 1261 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
 1321 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
 1381 cgatgcctac agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
 1441 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
 1501 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
 1561 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
 1621 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
 1681 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
 1741 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
 1801 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
 1861 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
 1921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
 1981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
 2041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
 2101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
 2161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
 2221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
 2281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
 2341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
 2401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
 2461 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
 2521 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
 2581 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
 2641 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
 2701 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
 2761 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
 2821 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
 2881 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
 2941 ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc
 3001 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
 3061 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
 3121 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
 3181 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
 3241 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
 3301 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
 3361 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
 3421 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
 3481 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
 3541 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
 3601 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
 3661 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
 3721 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
 3781 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
 3841 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
 3901 atcccgccgt caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
 3961 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
 4021 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
 4081 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
 4141 caacgcaatt aatgtgagtt agcgcgaatt gatctg
//

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pTrc 99a载体应用举例

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