| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pT7ts |
| GenBank ID: | AB255037 |
| 质粒类型: | 大肠杆菌表达载体 |
| 载体大小: | 3000 bp |
| 5' 测序引物及序列: | T7 |
| 3' 测序引物及序列: | SP6 |
| 载体抗性: | 氨苄青霉素 |
| 克隆方法: | 限制性内切酶 |
| 培养条件: | 37°C |
| 克隆菌株: | DH5α 等 |
买家导航
pT7ts载体质粒基本信息
pT7ts质粒图谱载体图谱和pT7ts载体序列质粒序列多克隆位点信息
pT7ts质粒载体简介
pT7ts质粒序列载体序列
LOCUS pT7ts 2965 bp ds-DNA circular SYN 03-OCT-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pT7TS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2965)
TITLE Krieg Lab plasmids
REFERENCE 2 (bases 1 to 2965)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 3, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2965
/organism="synthetic DNA construct"
/mol_type="other DNA"
3'UTR complement(62..187)
/label=Xenopus globin 3'-UTR
/note="3'-UTR of the major beta-globin gene of Xenopus
laevis"
5'UTR complement(233..275)
/label=Xenopus globin 5'-UTR
/note="translational enhancer from the 5'-UTR of the major
beta-globin gene of Xenopus laevis"
primer_bind complement(287..306)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(288..306)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(325..341)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(325..341)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(338..360)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 349..365
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(373..403)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 418..439
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(556..573)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(727..1315)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(807..826)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1486..2346)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2109..2128
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2347..2451)
/gene="bla"
/label=AmpR promoter
primer_bind 2519..2537
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2575..2597)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2697..2716
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2910..2932
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2924..2941
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2925..2941
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2949..2
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2949..1
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 gaatacgaat tcgagctcgc ccggggatcc tctagagtcg acctgcaggg ggggggggaa
61 tgtgaagaaa ctttcttttt attaggagca gatacgaatg gctacatttt gggggacaac
121 attttgtaaa gtgtaagttg gtattatgta gcttagagac tccattcggg tgttcttgag
181 gctggtttag tggtaaccag atcctagtca gtcactagtg atatcagatc tgccaaagtt
241 gagcgtttat tctgagcttc tgcaaaaaga acaagcaagc ttgtctccct atagtgagtc
301 gtattagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
361 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
421 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
481 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
541 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
1201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
1321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
1381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
1441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
1501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
1561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
1621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
1681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
1741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
1801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
1861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
1921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
1981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
2281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
2341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
2401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
2461 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
2521 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
2581 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
2641 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
2701 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
2761 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
2821 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
2881 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
2941 tgaattggat ttaggtgaca ctata
//
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