pSP73

价格:750元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Promega
载体名称: pSP73
质粒类型: 大肠杆菌克隆载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 2464bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: --
备注: --
产品目录号: P2221
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pSP73载体图谱



pSP73多克隆位点

pSP73载体特征

载体简介

The pSP73 Vector offers a wide range of restriction sites, providing greater versatility 
in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA 
polymerase promoters and a unique multiple cloning region, which includes restriction sites 
for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, 
PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation 
of the multiple cloning region.

载体序列

LOCUS       pSP73	2464 bp 	DNA	SYN
DEFINITION  pSP73
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="pSP73"
                     /mol_type="other DNA"
     promoter        complement(102..120)
                     /label="T7_promoter"
     rep_origin      complement(363..982)
                     /label="pBR322_origin"
     gene            complement(1137..1997)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1137..1997)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2039..2067)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(2226..2248)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2448..1
                     /label="Sp6_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gaaccagatc tgatatcatc gatgaattcg agctcggtac ccggggatcc tctagagtcg
   61 acctgcaggc atgcaagctt cagctgctcg aggccggtct ccctatagtg agtcgtatta
  121 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
  181 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
  241 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
  301 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
  361 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
  421 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
  481 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
  541 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
  601 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
  661 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
  721 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
  781 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
  841 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
  901 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
  961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
 1021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
 1081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
 1141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
 1201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
 1261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
 1321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
 1381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
 1441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
 1501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
 1561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
 1621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
 1681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
 1741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
 1801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
 1861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
 1921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
 1981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
 2041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
 2101 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
 2161 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
 2221 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
 2281 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
 2341 aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
 2401 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
 2461 tata
//

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