| 质粒名称: | pSL580 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000649 |
| 产品用途: | Mammalian cAMP-specific 8xANR-NSP3 expression vector (PCRE-(ANR)8-NSP3-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | pCRE |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | (ANR)8-NSP3 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pSL580
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL580载体质粒基本信息
pSL580质粒图谱载体图谱和pSL580载体序列质粒序列多克隆位点信息
pSL580质粒载体简介
pSL580质粒序列载体序列
LOCUS pSL580 5520 bp DNA circular SYN 14-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5520)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Jul 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5520
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(169..237)
/label=LacZ alpha
primer_bind 281..297
/label=oMH18
misc_feature 298..451
/label=Transcription blocker
/label=Transcription\blocker
misc_feature 485..571
/label=CRE reg_Regulatory_Seq
misc_feature 496..503
/label=CRE
misc_feature 532..539
/label=CRE*
misc_feature 554..561
/label=CRE
promoter 578..724
/label=P_TAL
primer_bind 681..708
/label=180-WKG-592-F
regulatory 733..742
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 741
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 742..804
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 805..879
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 880..942
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 943..1017
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1024..1086
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1087..1161
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1162..1224
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1225..1299
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1306..1368
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1369..1443
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1444..1506
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1507..1581
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1588..1650
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1651..1725
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1726..1788
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1789..1863
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 1870..2811
/label=NSP3
misc_feature 1870..1893
/label=NSP3
primer_bind complement(1881..1900)
/label=179-WKG-592-R
primer_bind complement(2860..2869)
/label=oMH18
polyA_signal 2910..3134
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature 3299..4239
/label=ColE1
/note="ColE1"
CDS complement(4240..5100)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5101..5205)
/label=AmpR promoter
rep_origin join(5232..5520,1..167)
/label=M13 origin
ORIGIN
1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
61 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
121 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt
181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
241 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
301 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
361 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
421 agtgcaagtg caggtgccag aacatttctc tatcgatagg taccgagctc ttacgcgtgc
481 tagcgcacca gacagtgacg tcagctgcca gatcccatgg ccgtcatact gtgacgtctt
541 tcagacaccc cattgacgtc aatgggagaa cagatctgcc gccccgactg catctgcgtg
601 ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga actaaagaca
661 tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc cacggggatg
721 aagcagaagc ttgccaccat gggggagctc gacgagttgg tttatctctt agacggaccc
781 ggatatgacc ccattcattc agatggctcc ggaacgggca gcgggaccgg aagtggcacc
841 ggaaccactt ccggaaccgg tactggcggg agtaccggcg gtgagctaga cgaactcgtg
901 tacctgttgg atggccccgg ttacgaccca atccacagcg atggaagtgg aactgggtct
961 gggactgggt ccggcaccgg gactacttca ggcaccggca ctgggggctc cactggccat
1021 accggggagc tcgacgagtt ggtttatctc ttagacggac ccggatatga ccccattcat
1081 tcagatggct ccggaacggg cagcgggacc ggaagtggca ccggaaccac ttccggaacc
1141 ggtactggcg ggagtaccgg cggtgagcta gacgaactcg tgtacctgtt ggatggcccc
1201 ggttacgacc caatccacag cgatggaagt ggaactgggt ctgggactgg gtccggcacc
1261 gggactactt caggcaccgg cactgggggc tccactggcc ataccgggga gctcgacgag
1321 ttggtttatc tcttagacgg acccggatat gaccccattc attcagatgg ctccggaacg
1381 ggcagcggga ccggaagtgg caccggaacc acttccggaa ccggtactgg cgggagtacc
1441 ggcggtgagc tagacgaact cgtgtacctg ttggatggcc ccggttacga cccaatccac
1501 agcgatggaa gtggaactgg gtctgggact gggtccggca ccgggactac ttcaggcacc
1561 ggcactgggg gctccactgg ccataccggg gagctcgacg agttggttta tctcttagac
1621 ggacccggat atgaccccat tcattcagat ggctccggaa cgggcagcgg gaccggaagt
1681 ggcaccggaa ccacttccgg aaccggtact ggcgggagta ccggcggtga gctagacgaa
1741 ctcgtgtacc tgttggatgg ccccggttac gacccaatcc acagcgatgg aagtggaact
1801 gggtctggga ctgggtccgg caccgggact acttcaggca ccggcactgg gggctccact
1861 ggccatatga tgctcaagat ggagtctaca cagcagatgg ctagttcgat tattaacact
1921 tcttttgaag ccgcagtcgt tgctgccact tcaactcttg aacttatggg cattcaatat
1981 gattataatg aagtttatac tagagttaag agcaaatttg attacgtaat ggatgattca
2041 ggtgttaaaa acaatctgtt gggtaaagcg gcaactattg atcaggcact gaatggaaaa
2101 tttggttcag cagcaagaaa tagaaattgg atggctgata ctagaactac agctagacta
2161 gatgaagatg tgaacaaact tagaatgatg ctatcatcta aaggaattga tcaaaaaatg
2221 agagtactta atgcatgttt caatgtgaag cgagttccag gtaaatcatc atcaataatt
2281 aaatgtacta gattaatgag agataagatt gaacgtggtg aagtcgaagt ggatgattcg
2341 tttgttgaag aaaaaatgga agttgacaca attgattgga aatctagata tgaacaattg
2401 gaaaaacgat ttgaatcgct gaaacaaagg gtgaatgaaa aatatacaag ttgggtgcaa
2461 aaagcaaaaa aggtaaatga aaatatgtac tctctccaaa acgttatatc acaacagcag
2521 agccagattg ctgatttaca aaattactgt aataaattgg aagttgatct acaaaataaa
2581 attagttcac ttgtgtcttc agtcgaatgg tacctgaaat caatggaatt gcctgatgaa
2641 attaagacag atattgaaca acaactaaat tccattgatg taatcaatcc aattaatgct
2701 atagatgatt ttgaatcact gatcagaaat attatactcg actatgacag aatattttta
2761 atgtttaaag gattaatgag gcaatgcaac tatgaatata cgtatgaata ggatccacta
2821 gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
2881 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
2941 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
3001 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
3061 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
3121 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg tcgaccgatg
3181 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
3241 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
3301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
3361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
3421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
3481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
3541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
3601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
3661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
3721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
3781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
3841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
3901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
3961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
4021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
4081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
4141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
4201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
4261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
4321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
4381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
4441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
4501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
4561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
4621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
4681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
4741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
4801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
4861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
4921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
4981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
5041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
5101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
5161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
5221 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
5281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
5341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
5401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
5461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
//
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