| 质粒名称: | pCMV-PEmax |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 174820 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 9795 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PEmax |
| 物种来源: | 合成的SpCas9来自化脓性链球菌；MMLV_RT来自莫罗尼小鼠白血病病毒 |
| 插入大小: | 6393 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | SV40 bpNLS（插入件上的N端子）SV40 bpNLS（插入件上的C端子）c-Myc NLS（插入件上的c端子） |
| 克隆方法: | Ligation Independent Cloning |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7-forward |
| 3’测序引物 | BGH-reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-PEmax was a gift from David Liu (Addgene plasmid # 174820 ; http://n2t.net/addgene:174820 ; RRID:Addgene_174820) |
| 参考文献部分: | Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, Liu DR. Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 10.1016/j.cell.2021.09.018. 10.1016/j.cell.2021.09.018 PubMed 34653350 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-PEmax 9795 bp ds-DNA circular SYN 23-JUN-2023
DEFINITION Mammalian expression of SpCas9 PEmax prime editor.
ACCESSION .
VERSION .
KEYWORDS pCMV-PEmax
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9795)
AUTHORS Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen
C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B,
Liu DR
TITLE Enhanced prime editing systems by manipulating cellular determinants
of editing outcomes.
JOURNAL Cell. 2021 Oct 28;184(22):5635-5652.e29. doi:
10.1016/j.cell.2021.09.018.
PUBMED 34653350
REFERENCE 2 (bases 1 to 9795)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 23, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9795
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 27..47
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 57..83
/codon_start=1
/product="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
/label=c-myc NLS
/translation="PAAKRVKLD"
CDS 92..109
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(132..149)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 138..362
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(433..449)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(433..449)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(446..468)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 457..473
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(481..511)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 526..547
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(664..681)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(835..1423)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(915..934)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1594..2454)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2217..2236
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2455..2559)
/gene="bla"
/label=AmpR promoter
primer_bind complement(2638..2657)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2829..3208
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3209..3412
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3363..3383
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3454..3473
/label=T7
/note="T7 promoter, forward primer"
promoter 3454..3472
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3522..3542
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3543..7643
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 7701..7721
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
ORIGIN
1 ccgccgacgg cagcgaattc gagtctccca agaagaagag gaaagtcggc tctggccctg
61 ccgctaagag agtgaagctg gactaaccgg tcatcatcac catcaccatt gagtttaaac
121 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
181 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagaa
241 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
301 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
361 ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt
421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
481 caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact
541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
1021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
1081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
1141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
1201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
1261 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
1321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
1381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
1441 ttctacgggg tctgacactc agtggaacga aaactcacgt taagggattt tggtcatgag
1501 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
1561 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
1621 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
1681 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
1741 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
1801 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
1861 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
1921 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
1981 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
2041 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
2101 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
2161 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
2221 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
2281 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
2341 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
2401 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
2461 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
2521 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
2581 acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac
2641 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
2701 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
2761 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
2821 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
2881 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
2941 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
3001 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
3061 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
3121 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
3181 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
3241 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
3301 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
3361 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
3421 gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg
3481 ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag
3541 tcgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
3601 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
3661 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg
3721 ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
3781 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
3841 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
3901 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
3961 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
4021 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
4081 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca
4141 tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagaa
4201 agctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc
4261 tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg
4321 atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc
4381 agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc
4441 tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta
4501 tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc
4561 agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg
4621 gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg
4681 aaaagatgga cggcaccgag gaactgctcg tgaagctgaa gagagaggac ctgctgcgga
4741 agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg
4801 ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg
4861 agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca
4921 gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag
4981 tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga
5041 acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt
5101 ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga
5161 gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg
5221 tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct
5281 ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta
5341 tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc
5401 tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc
5461 acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca
5521 ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg
5581 atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca
5641 gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc
5701 acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag
5761 tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga
5821 tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa
5881 tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg
5941 tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg
6001 atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat gtggacgcta
6061 tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg
6121 acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga
6181 actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga
6241 ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc
6301 tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca
6361 ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca
6421 agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact
6481 accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt
6541 accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga
6601 tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca
6661 acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc
6721 ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg
6781 ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc
6841 agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg
6901 ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct
6961 attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga
7021 aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact
7081 ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt
7141 actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc
7201 agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc
7261 actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac
7321 agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga
7381 tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc
7441 ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc
7501 ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag
7561 aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg
7621 acctgtctca gctgggaggt gactccggcg gaagctctgg tggcagcaag cggaccgccg
7681 acggctctga attcgagagc cctaagaaga aaagaaaggt gagcggaggc tctagcggcg
7741 gaagcaccct gaacattgaa gacgagtata gactgcatga aacaagcaag gaacccgacg
7801 tgtccctggg ctccacctgg ctgtccgact ttccccaggc ctgggccgag acaggaggaa
7861 tgggcctggc cgtgcggcag gcacccctga tcatccctct gaaggccacc tctacacccg
7921 tgagcatcaa gcagtaccct atgtctcagg aggccagact gggcatcaag cctcacatcc
7981 agaggctgct ggaccagggc atcctggtgc catgccagag cccctggaac acaccactgc
8041 tgcccgtgaa gaagccaggc accaatgact atagacccgt gcaggatctg agagaggtga
8101 acaagagggt ggaggatatc caccccaccg tgcccaaccc ttacaatctg ctgtccggcc
8161 tgcccccttc tcaccagtgg tatacagtgc tggacctgaa ggatgccttc ttttgtctga
8221 gactgcaccc taccagccag ccactgttcg cctttgagtg gagggaccct gagatgggca
8281 tctctggcca gctgacctgg acacgcctgc ctcagggctt caagaatagc ccaacactgt
8341 ttaacgaggc cctgcaccgc gacctggcag atttccggat ccagcaccca gatctgatcc
8401 tgctgcagta cgtggacgat ctgctgctgg ccgccaccag cgagctggat tgccagcagg
8461 gaacacgcgc cctgctgcag accctgggaa acctgggata tagggcatcc gccaagaagg
8521 cccagatctg tcagaagcag gtgaagtacc tgggctatct gctgaaggag ggccagagat
8581 ggctgacaga ggccaggaag gagacagtga tgggccagcc aacacccaag accccaagac
8641 agctgaggga gttcctgggc aaagcaggat tttgcaggct gttcatccca ggattcgcag
8701 agatggcagc acctctgtac ccactgacca agccgggcac cctgtttaat tggggccctg
8761 accagcagaa ggcctatcag gagatcaagc aggccctgct gacagcacca gccctgggcc
8821 tgccagacct gaccaagcct ttcgagctgt ttgtggatga gaagcagggc tacgccaagg
8881 gcgtgctgac ccagaagctg ggaccatgga gacggcccgt ggcctatctg tccaagaagc
8941 tggacccagt ggcagcagga tggccaccat gcctgaggat ggtggcagca atcgccgtgc
9001 tgacaaagga tgccggcaag ctgaccatgg gacagccact ggtcatcctg gcaccacacg
9061 cagtggaggc cctggtgaag cagcctccag atcgctggct gtctaacgcc cggatgacac
9121 actaccaggc cctgctgctg gacaccgatc gcgtgcagtt tggccctgtg gtggccctga
9181 atccagccac cctgctgcct ctgccagagg agggcctgca gcacaactgt ctggacatcc
9241 tggcagaggc acacggaaca aggccagacc tgaccgatca gcccctgcct gacgccgatc
9301 acacatggta taccgatgga agctccctgc tgcaggaggg ccagaggaag gcaggagcag
9361 cagtgaccac agagacagaa gtgatctggg ccaaggccct gccagcaggc acatccgccc
9421 agcgggccga gctgatcgcc ctgacccagg ccctgaagat ggccgagggc aagaagctga
9481 acgtgtacac agactccaga tatgccttcg ccaccgcaca catccacgga gagatctaca
9541 ggcgccgggg ctggctgacc tctgagggca aggagatcaa gaacaaggat gagatcctgg
9601 ccctgctgaa ggccctgttt ctgcccaagc ggctgagcat catccactgt cctggacacc
9661 agaagggaca ctccgccgag gcaaggggca atcggatggc cgaccaggcc gccagaaagg
9721 ctgctattac tgaaactccc gacacttcca ctctgctgat tgaaaactcc tccccttctg
9781 gcggctcaaa aagaa
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：RBD#3L
- 下一篇：pcDNA3-TORCAR
