| 质粒名称: | pcDNA4TO-mito-mCherry-24xGCN4_v1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60913 |
| 存储实验室: | Ron Vale |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA4TO |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mito-mCherry |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA4TO-mito-mCherry-24xGCN4_v1 was a gift from Ron Vale (Addgene plasmid # 60913 ; http://n2t.net/addgene:60913 ; RRID:Addgene_60913) |
| 参考文献部分: | A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA4TO-mito-mCherry-24xGCN4_v1 8025 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION A fusion protein of the SunTag 24xGCN4 v1 peptide array to a
mitochondrial targeting domain and mCherry.
ACCESSION .
VERSION .
KEYWORDS pcDNA4TO-mito-mCherry-24xGCN4_v1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8025)
AUTHORS Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
TITLE A Protein-Tagging System for Signal Amplification in Gene Expression
and Fluorescence Imaging.
JOURNAL Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi:
10.1016/j.cell.2014.09.039.
PUBMED 25307933
REFERENCE 2 (bases 1 to 8025)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8025
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 720..740
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
promoter 725..769
/label=CMVd3 promoter
/note="truncated human cytomegalovirus (CMV) immediate
early promoter that yields strongly reduced expression
relative to the full-length promoter"
protein_bind 771..789
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 792..810
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 820..844
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 1163..1867
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(1305..1323)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1573..1592
/label=mCherry-F
/note="mCherry, forward primer"
CDS 1955..2020
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2036..2101
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2117..2182
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2198..2263
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2279..2344
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2360..2425
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2441..2506
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2522..2587
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2603..2668
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2684..2749
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2765..2830
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 2846..2911
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
primer_bind 2927..2949
/label=pGEX 5'
/note="pGEX vectors, Glutathione-S-transferase, forward
primer"
primer_bind 2950..2972
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS 2975..3040
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3056..3121
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3137..3202
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3218..3283
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3299..3364
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3380..3445
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3461..3526
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3542..3607
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3623..3688
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3704..3769
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3785..3850
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
CDS 3866..3931
/codon_start=1
/product="GCN4 peptide that allows binding to an scFv-GFP
fusion protein (Tanenbaum et al., 2014)"
/label=GCN4_v1
/translation="LLPKNYHLENEVARLKKLVGER"
primer_bind complement(4036..4053)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4042..4266
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4312..4740
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4399..4418)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4609..4630
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4749..4769)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4754..5083
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4934..5069
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4996..5015
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5131..5178
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 5197..5571
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 5701..5822
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5738..5757)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5792..5811
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5871..5887)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5871..5887)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5884..5906)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5895..5911
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5919..5949)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5964..5985
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6102..6119)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6273..6858)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6353..6372)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7029..7889)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7652..7671
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7890..7994)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcag ctcagctttc tagaccgcgc
721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
781 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
841 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
901 cagcctccgg actctagcgt ttaaacttaa gcttggtacc gagctcggat ccgccaccat
961 gagtctgact tccagttcca gcgtacgagt tgaatggatc gcagcagtta ccattgctgc
1021 tgggacagct gcaattggtt atctagctta caaaagattt tatgttaaag atcatcgaaa
1081 taaagctatg ataaaccttc acatccagaa agacaacccc aagatagtac atgctggaag
1141 cttaccggtc gccgaattcg gtgtgagcaa gggcgaggag gataacatgg ccatcatcaa
1201 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
1261 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
1321 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
1381 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
1441 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
1501 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
1561 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
1621 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
1681 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
1741 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
1801 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
1861 gtacaagggt ggtggtggcg cggccgcaga tcgatcgatc acgcgtgcca ccatgactag
1921 ttttgtacaa agcggtccgg gaggttctgg aggattgttg cccaaaaact accacttgga
1981 gaacgaggtc gcaaggttga aaaaattggt cggtgagcgt ggcggctcag gcggccttct
2041 tccaaaaaat tatcatcttg aaaatgaagt tgctagactt aaaaaacttg ttggagaaag
2101 aggcgggagc ggcggcctgc tgcccaagaa ctaccacctg gagaacgagg tcgcccgcct
2161 gaagaagctg gtcggcgagc gcggtggttc tggaggtttg ctcccaaaga actaccactt
2221 ggaaaacgaa gtcgcaagat tgaagaagtt ggtcggtgaa agaggaggaa gcggaggatt
2281 attacctaaa aattatcatt tggaaaatga agttgctaga ttgaagaagt tggttggaga
2341 gagagggggg tctggaggac tactaccgaa aaactaccac ctagagaacg aggtggcccg
2401 actaaaaaag ctagtgggcg agcgcggcgg cagtggcggc ctcctcccca agaattacca
2461 cctcgaaaat gaagtagcaa ggctcaagaa gctcgtagga gaaagaggtg gttccggggg
2521 actccttcca aaaaactatc atcttgaaaa cgaagttgca cgtcttaaaa aacttgttgg
2581 cgaacgtggc ggctctggcg ggctgctgcc caagaattac cacctggaaa atgaagtggc
2641 ccgcctgaag aagctggtgg gcgaacgcgg aggcagtgga ggcctactac cgaagaacta
2701 ccacctagag aacgaggtgg cacgcctaaa gaagctagtg ggcgagcgcg gcggctcggg
2761 tggtttatta cctaagaatt atcatttaga aaatgaagta gctcgattaa agaagttagt
2821 aggtgaacgt ggtgggtctg gtggtcttct tccgaagaac taccaccttg agaacgaggt
2881 agctcgtctt aagaaacttg taggtgagcg tggtggaagc ggtggtgggc tggcaagcca
2941 cgtttggtgc cgggagctgc atgtgtcaga ggaacttttg ccaaagaatt atcatcttga
3001 gaacgaagtg gctcgtctta agaaactcgt aggagaaaga ggtggcagtg gaggtctgct
3061 tcccaagaat taccacctgg aaaatgaggt agctagactg aaaaagctcg taggcgagcg
3121 cgggggaagt gggggattgc tgccaaaaaa ttatcatttg gagaacgaag tagcacgact
3181 aaagaaatta gttggggaga gggggggatc gggtggctta ctcccgaaaa attatcatct
3241 cgaaaacgaa gtggctcggc taaaaaagtt agttggagag cgtggcggtt ctggaggcct
3301 attaccaaaa aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagc tggtcggaga
3361 acggggaggc agtggtggcc tactacccaa gaattatcat cttgagaacg aggtagcgcg
3421 tttgaagaag ctcgtcggtg aaagaggtgg ctcaggaggg ctgctcccaa agaactatca
3481 tcttgaaaat gaggtcgctc gattaaaaaa gttagtgggc gagaggggag gcagtggtgg
3541 gctacttcct aagaattacc acctcgaaaa cgaagtagct cgattaaaga aattggtcgg
3601 tgagagaggt gggtcgggtg gcttactgcc gaaaaattat catctggaaa atgaggtagc
3661 gagactaaaa aagctcgtag gtgagagagg gggttctggc ggcttgctac caaaaaatta
3721 tcatcttgag aacgaagttg ctaggctcaa aaagctggtt ggagagcgcg gcggctcagg
3781 cggtctgtta cccaaaaact accacttgga aaatgaggtt gccagattga agaaactggt
3841 cggcgagcgc ggtggaagtg gaggactact tcctaaaaat tatcatttgg agaacgaagt
3901 cgctagactg aaaaagttgg ttggggaacg aggagggtct ggcggcaagc tagcgatcgg
3961 atcgatcgcc gcggtaaaca tatggatcga tcataatact cgagtctaga gggcccgttt
4021 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
4081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
4141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
4201 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
4261 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
4681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4741 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
4801 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
4861 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
4921 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
4981 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
5041 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
5101 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
5161 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
5221 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
5281 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
5341 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
5401 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
5461 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
5521 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
5581 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
5641 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
5701 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
5761 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
5821 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
5881 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
5941 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
6001 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
6061 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
6121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
6181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
6241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
6301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
6361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
6421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
6481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
6541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
6601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
6661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
6721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
6781 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
6841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
6901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
6961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
7021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
7081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
7141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
7201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
7261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
7321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
7381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
7441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
7501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
7561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
7621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
7681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
7741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
7801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
7861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
7921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
7981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
