| 质粒名称: | pCMV-Bx |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 51552 |
| 存储实验室: | Michele Calos |
|
载体骨架基本信息 |
|
| 载体名称: | pCMVSPORTbetaGal |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Bxb1 integrase |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-Bx was a gift from Michele Calos (Addgene plasmid # 51552 ; http://n2t.net/addgene:51552 ; RRID:Addgene_51552) |
| 参考文献部分: | DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, Chen-Tsai Y, Calos MP. Nucleic Acids Res. 2013 Dec 4. 10.1093/nar/gkt1290 PubMed 24304893 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-Bx 5803 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses Bxb1 integrase in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS pCMV-Bx
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5803)
AUTHORS Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B,
Chen-Tsai Y, Calos MP
TITLE DICE, an efficient system for iterative genomic editing in human
pluripotent stem cells.
JOURNAL Nucleic Acids Res. 2013 Dec 4.
PUBMED 24304893
REFERENCE 2 (bases 1 to 5803)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5803
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 2..457
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(89..108)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 299..320
/label=F1ori-F
/note="F1 origin, forward primer"
CDS 745..1605
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(963..982)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
protein_bind complement(1707..1740)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin 1818..2406
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2307..2326
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2560..2577
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2907..2923
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2912..2934
/label=M13/pUC Reverse
/note="In lacZ gene"
enhancer 2953..3256
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3257..3460
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3411..3431
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3457..3481
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 3576..3594
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 3576..3593
/label=SP6
/note="SP6 promoter, forward primer"
polyA_signal 5546..5680
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5583..5602)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5637..5656
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 ccaggtcttg gactggtgag aacggcttgc tcggcagctt cgatgtgtgc tggagggaga
601 ataaaggtct aagatgtgcg atagagggaa gtcgcattga attatgtgct gtgtagggat
661 cgctggtatc aaatatgtgt gcccacccct ggcatgagac aataaccctg ataaatgctt
721 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
781 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
841 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
901 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
961 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
1021 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
1081 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
1141 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
1201 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
1261 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
1321 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
1381 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
1441 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
1501 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
1561 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
1621 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
1681 aagatccttt ttgataatct catgccataa cttcgtataa tgtatgctat acgaagttat
1741 ggcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
1801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
1861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
1921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
1981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
2041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
2101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
2161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
2221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
2281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
2341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
2401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
2461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
2521 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
2581 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
2641 agagcttgca attcgcgcgt ttttcaatat tattgaagca tttatcaggg ttattgtctc
2701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
2761 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
2821 aaaaataggc gtatacgagg ccctttcact cattaggcac cccaggcttt acactttata
2881 gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
2941 catcgtgcag gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
3001 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
3061 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
3121 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
3181 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
3241 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
3301 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
3361 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
3421 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
3481 cgcctggagg cgccatccac gctgttttga cctccataga agacaccggg accgatccag
3541 cctccggact ctagcctagg cttttgcaaa aagctattta ggtgacacta tagaaggtac
3601 gcctgcaggt accggtccgg aattcgccct tatgagagcc ctggtagtca tccgcctgtc
3661 ccgcgtcacc gatgctacga cttcaccgga gcgtcagctg gagtcttgcc agcagctctg
3721 cgcccagcgc ggctgggacg tcgtcggggt agcggaggat ctggacgtct ccggggcggt
3781 cgatccgttc gaccggaagc gcagaccgaa cctggcccgg tggctagcgt tcgaggagca
3841 accgttcgac gtgatcgtgg cgtaccgggt agaccggttg acccgatcga tccggcatct
3901 gcagcagctg gtccactggg ccgaggacca caagaagctg gtcgtctccg cgaccgaagc
3961 gcacttcgat acgacgacgc cgtttgcggc ggtcgtcatc gcgcttatgg gaacggtggc
4021 gcagatggaa ttagaagcga tcaaagagcg gaaccgttcg gctgcgcatt tcaatatccg
4081 cgccgggaaa taccgaggat ccctgccgcc gtggggatac ctgcctacgc gcgtggacgg
4141 ggagtggcgg ctggtgccgg accctgtgca gcgagagcgc atcctcgagg tgtatcaccg
4201 cgtcgtcgac aaccacgagc cgctgcacct ggtggcccac gacctgaacc ggcgtggtgt
4261 cctgtcgccg aaggactact tcgcgcagct gcaaggccgc gagccgcagg gccgggagtg
4321 gtcggctacc gcgctgaagc gatcgatgat ctccgaggcg atgctcgggt acgcgactct
4381 gaacggtaag accgtccgag acgacgacgg agccccgctg gtgcgggctg agccgatcct
4441 gacccgtgag cagctggagg cgctgcgcgc cgagctcgtg aagacctccc gggcgaagcc
4501 cgcggtgtct accccgtcgc tgctgctgcg ggtgttgttc tgcgcggtgt gcggggagcc
4561 cgcgtacaag ttcgccgggg gaggacgtaa gcacccgcgc taccgctgcc gctcgatggg
4621 gttcccgaag cactgcggga acggcacggt ggcgatggcc gagtgggacg cgttctgcga
4681 ggagcaggtg ctggatctgc tcggggacgc ggagcgtctg gagaaagtct gggtagccgg
4741 ctcggactcc gcggtcgaac tcgcggaggt gaacgcggag ctggtggacc tgacgtcgct
4801 gatcggctcc ccggcctacc gggccggctc tccgcagcga gaagcactgg atgcccgtat
4861 tgcggcgctg gccgcgcggc aagaggagct ggagggtcta gaggctcgcc cgtctggctg
4921 ggagtggcgc gagaccgggc agcggttcgg ggactggtgg cgggagcagg acaccgcggc
4981 aaagaacacc tggcttcggt cgatgaacgt tcggctgacg ttcgacgtcc gcggcgggct
5041 gactcgcacg atcgacttcg gggatctgca ggagtacgag cagcatctca ggctcggcag
5101 cgtggtcgaa cggctacaca ccgggatgtc gtagaagggc gaattcccgg gtcgacgagc
5161 tcactagctt gggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
5221 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
5281 taaactagct gcatatgctt gctgcttgag agttttgctt actgagtatg atttatgaaa
5341 atattataca caggagctag tgattctaat tgtttgtgta ttttagattc acagtcccaa
5401 ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca gccataccac
5461 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
5521 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
5581 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
5641 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatcctgcat taatgaatcg
5701 gccaacgcgc ggggagaggc ggtttgcgta ttggctggcg taatagcgaa gaggcccgca
5761 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atg
//
