| 质粒名称: | pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 154223 |
| 存储实验室: | Arnold Driessen |
|
载体骨架基本信息 |
|
| 载体名称: | pAGM4673 |
| 载体出品公司: | MoClo Tool Kit of Sylvestre Marillonnet |
| 空载体大小: | 8097 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，合成生物学；真菌表达 |
| 筛选标记: | amdS fungal selection |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | p40S:QfDBD-VP16AD-GFP-NLS,P(various):DsRed-SKL,pGpdA:amdS |
| 物种来源: | 合成的真菌表达，红曲霉（原名产黄曲霉） |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | p40S（AN0465巢状按蚊） |
| 标签/融合蛋白: | GFP-NLS；DsRed SKL（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS was a gift from Arnold Driessen (Addgene plasmid # 154223 ; http://n2t.net/addgene:154223 ; RRID:Addgene_154223) |
| 参考文献部分: | Synthetic control devices for gene regulation in Penicillium chrysogenum. Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y. Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 10.1186/s12934-019-1253-3. 10.1186/s12934-019-1253-3 PubMed 31739777 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS 14353 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor,
activating DsRed-SKL with promoter 11xQUAS+CP_pcbC.
ACCESSION .
VERSION .
KEYWORDS pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14353)
AUTHORS Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
TITLE Synthetic control devices for gene regulation in Penicillium
chrysogenum.
JOURNAL Microb Cell Fact. 2019 Nov 18;18(1):203. doi:
10.1186/s12934-019-1253-3.
PUBMED 31739777
REFERENCE 2 (bases 1 to 14353)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14353
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2781..2891
/codon_start=1
/product="3 tandem repeats of the minimal activation domain
of herpes simplex virus VP16 (Cheng et al., 2013)"
/label=VP48
/translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
CDS 2904..3620
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2904..3620
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2949..2970)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3210..3229)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3557..3578
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3621..3641
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 4813..4928
/gene="gpdA"
/label=gpdA intron
/note="intron from the Aspergillus nidulans
glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
1990)"
CDS 7623..8297
/codon_start=1
/product="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
/label=DsRed-Express
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
LFL"
primer_bind complement(7753..7771)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind complement(7803..7823)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 8215..8238
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 10131..10155
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 10243..10953
/label=oriV
/note="incP origin of replication"
promoter 10974..11078
/gene="bla"
/label=AmpR promoter
CDS 11079..11873
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 12044..12632
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 12533..12552
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 12968..14116
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
misc_feature 14262..14286
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
6241 cataccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
7201 cgaattccca tggggagggg taatcgatta tcctcggata aacacttatc ccagggtaat
7261 cacttatccc tggataaaca attatcctcg ggtaatcgct tatcctacgg ggtaatcgct
7321 tatccttgcg ggtaatcgct tatcctcgga taaacaatta tcccagggta atcacttatc
7381 cctggataaa caattatcct cgggtaatcg cttatcccca tcgtataatg tctccaggtt
7441 gtctcagcat aaacaccccg cccccgctca ggcacacagg aagagagctc aggtcgtttc
7501 cattgcgtcc atactcttca ctcattgtca tctgcaggag aacttcccct gtccctttgc
7561 caagccctct cttcgtcgtt gtccacgcct tcaagttttc accattattt ttctagacac
7621 caatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
7681 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
7741 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
7801 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
7861 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
7921 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc tccttcatct
7981 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaaa
8041 ctatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
8101 agatccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca
8161 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac tccaagctgg
8221 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc gccgagggcc
8281 gccaccacct gttcctgact cacggtatgg acgaattgta caagtcgaag ctgtaagctt
8341 gtgcttctaa ggtatgagtc gcaaaattgt tttttatttt tggtcttgag tctaatatgc
8401 tcgcagctct tgcgttgtat atggtcgttg tcgcgtattt tctgttgtat taaaagatca
8461 aacgagatca agggatggct cgcgggctgt ctctcgcact aggaggaaga atgcctgaaa
8521 aaggaacttt gattttagct gtggaataga gatggcttgt ttgaggacgc ttgtcgcttg
8581 gcgcagggac ttgaatggca gcttgtggaa accgaaggcg agaaaagtcg acggatactg
8641 tacgtggttc tattgccagt gcggtggaag cttccttctc atatagttca atccttcttt
8701 gaatctgttt gtttcatatt tggactgttt cattctctgc ttgcgcattc tcatcttcga
8761 gaacgactgc agggattgtt ggttctgtgg agctgatgag cgcgccttga ccacccttgt
8821 tcttgttttg ctcttttgtt ctcatttaac ccgtttctcc cttccaaccc tttgaccttg
8881 caacattgtc tcccagcgcg ttgccaaagc gaacttgata tcagtatagt atgaccaagt
8941 agtctaccaa aataaatttt agtacagtat tgctagttat acaagataaa ttttgtacaa
9001 caatgattat gcagagctgg tattctgtaa ggagtaaatt catgcaggta cagagcgtcc
9061 acgtcaggca cacgcgcttt agtgctgggt acacattatg ctacctttgg ggcaaggtgc
9121 tattctaggt agggtatgcc tagcaatgcc atgatcttat gacctcagta atctgacctt
9181 ggatttaaga tcggagttct caaaccctgc gcgctcagag aattcgcatg cggagacttt
9241 agacatacct ctcgttgccg acaaatcggc attccgtttc ggtcacggac gtaagagttc
9301 aacaaagccg ctcacctgtc ccgttcggcg cttgagtttc cacttcaaaa gcacaaccgc
9361 tccgatattt ccattgccac cgaccaacaa aaggttcata tcgcgcaata gtcgtccaac
9421 tctcactccg cccgcactcg atggcgaagg atggaagaat atccgagcgg catcggataa
9481 agagatctgg ttcttttcgt gaagtaacgt agggaaccga cggaaactgt agagctatag
9541 gaatttagta tatcagaagt caattgagag aagtgtatag ttagtaactc acttgtgcca
9601 acaggtattt ccaaccgttc ttgttcaaat ttcgtcaagt caccatctct atgtgcaaaa
9661 ctggatcata caacagctct tattgtagct gcatctcgct ctcagagatc atcctatctt
9721 cgtcgtcatc caccaacaca ggtgggagtg ctagttgatc catgctgagg tcaagggaca
9781 cagacggaat gggtgtagaa cgcctcaaaa caggtaaggt atcaggatcg gttgtccagg
9841 gggaatccaa accaggttcc accttgattc agagaataag tatgacgtat tcttggcaat
9901 gtttaagctt gagatgagcc gatgtcaaac tgggagacat gatgaaggag gaaatgttgc
9961 tttggtgggg gagacactat aaataccaaa gagtgaatgg ttgataccat ttggggaagg
10021 agtgtctatc gcagtctctg cagacggcat atcggatagc ctgcaggcgc ttgtggagga
10081 tgcacatgtg accgagggac acgaagtgat ccgtttaaac tatcagtgtt tgacaggata
10141 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
10201 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca gccgcctttg cgacgctcac
10261 cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
10321 aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga
10381 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
10441 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
10501 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
10561 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
10621 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
10681 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
10741 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
10801 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
10861 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct
10921 cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc caattcccga gtgcgcggaa
10981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
11041 cctgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg agaatatcac
11101 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
11161 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
11221 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
11281 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
11341 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
11401 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
11461 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
11521 ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca tttaaagatc
11581 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
11641 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
11701 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcgc
11761 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
11821 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagctgtcag
11881 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
11941 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
12001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
12061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
12121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
12181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
12241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
12301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
12361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
12421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
12481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
12541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
12601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
12661 tcctggcaga tcctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt
12721 cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg
12781 gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac
12841 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg
12901 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg
12961 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg
13021 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga
13081 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag
13141 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg
13201 tcttgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat
13261 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa
13321 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt
13381 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc
13441 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt
13501 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga
13561 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat
13621 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta
13681 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga
13741 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg
13801 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg
13861 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg
13921 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg
13981 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc
14041 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt
14101 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagcccctgc
14161 tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc gagtggtgat
14221 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
14281 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg
14341 ggttgaacac tct
//
