| 质粒名称: | mEos3.2-Tubulin-C-18 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 57484 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mEos3.2-C1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tubulin,NM_006082.2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | mEos3.2（主干上的N终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mEos3.2-Tubulin-C-18 was a gift from Michael Davidson (Addgene plasmid # 57484 ; http://n2t.net/addgene:57484 ; RRID:Addgene_57484) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mEos3.2-Tubulin-C-18 6042 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Localization: Microtubules, Excitation: 507 / 572, Emission: 516 /
580.
ACCESSION .
VERSION .
KEYWORDS mEos3.2-Tubulin-C-18
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6042)
TITLE Davidson Photoactivatable Fluorescent Proteins
REFERENCE 2 (bases 1 to 6042)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6042
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 620..1297
/codon_start=1
/product="improved monomeric variant of green-to-red
photoswitchable fluorescent protein EosFP (Zhang et al.,
2012)"
/label=mEos3.2
/translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
NARR"
polyA_signal 2837..2958
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2874..2893)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2928..2947
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2965..3420)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3102..3123)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3314..3333
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3447..3551
/gene="bla"
/label=AmpR promoter
promoter 3553..3910
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3576..3596)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3761..3896
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3823..3842
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3945..4739
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3999..4018)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4609..4628
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4927..4946)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4971..5018
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5347..5935
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5836..5855
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gtcgccacca tgagtgcgat taagccagac atgaagatca aactccgtat
661 ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt
721 tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt
781 tgatatcctg accactgcat tccattacgg caacagggta ttcgccaaat atccagacaa
841 catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac
901 tttcgaagac gggggcattt gcaacgccag aaacgacata acaatggaag gggacacttt
961 ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa
1021 gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac
1081 gggtgatatt gagatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag
1141 aactacttac aaagctaagg agaagggtgt caagttacca ggcgcccact ttgtggacca
1201 ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc
1261 tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggactcagat ctggcagcgg
1321 tggaggcagc gcatccggcg gaagcggaag cgtgcgtgag tgcatctcca tccacgttgg
1381 ccaggctggt gtccagattg gcaatgcctg ctgggagctc tactgcctgg aacacggcat
1441 ccagcccgat ggccagatgc caagtgacaa gaccattggg ggaggagatg actccttcaa
1501 caccttcttc agtgagacgg gcgctggcaa gcacgtgccc cgggctgtgt ttgtagactt
1561 ggaacccaca gtcattgatg aagttcgcac tggcacctac cgccagctct tccaccctga
1621 gcagctcatc acaggcaagg aagatgctgc caataactat gcccgagggc actacaccat
1681 tggcaaggag atcattgacc ttgtgttgga ccgaattcgc aagctggctg accagtgcac
1741 cggtcttcag ggcttcttgg ttttccacag ctttggtggg ggaactggtt ctgggttcac
1801 ctccctgctc atggaacgtc tctcagttga ttatggcaag aagtccaagc tggagttctc
1861 catttaccca gcaccccagg tttccacagc tgtagttgag ccctacaact ccatcctcac
1921 cacccacacc accctggagc actctgattg tgccttcatg gtagacaatg aggccatcta
1981 tgacatctgt cgtagaaacc tcgatatcga gcgcccaacc tacactaacc ttaaccgcct
2041 tattagccag attgtgtcct ccatcactgc ttccctgaga tttgatggag ccctgaatgt
2101 tgacctgaca gaattccaga ccaacctggt gccctacccc cgcatccact tccctctggc
2161 cacatatgcc cctgtcatct ctgctgagaa agcctaccat gaacagcttt ctgtagcaga
2221 gatcaccaat gcttgctttg agccagccaa ccagatggtg aaatgtgacc ctcgccatgg
2281 taaatacatg gcttgctgcc tgttgtaccg tggtgacgtg gttcccaaag atgtcaatgc
2341 tgccattgcc accatcaaaa ccaagcgcag catccagttt gtggattggt gccccactgg
2401 cttcaaggtt ggcatcaact accagcctcc cactgtggtg cctggtggag acctggccaa
2461 ggtacagaga gctgtgtgca tgctgagcaa caccacagcc attgctgagg cctgggctcg
2521 cctggaccac aagtttgacc tgatgtatgc caagcgtgcc tttgttcact ggtacgtggg
2581 tgaggggatg gaggaaggcg agttttcaga ggcccgtgaa gatatggctg cccttgagaa
2641 ggattatgag gaggttggtg tggattctgt tgaaggagag ggtgaggaag aaggagagga
2701 atactaagga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
2761 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
2821 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
2881 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
2941 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
3001 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
3061 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
3121 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
3181 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
3241 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
3301 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
3361 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
3421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
3481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
3541 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
3601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
3661 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3721 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
3781 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
3841 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
3901 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
3961 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
4021 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
4081 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
4141 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
4201 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
4261 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
4321 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
4381 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
4441 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
4501 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
4561 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
4621 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
4681 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
4741 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
4801 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
4861 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
4921 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
4981 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
5041 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
5101 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
5161 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
5221 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
5281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
5341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
5401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
5461 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
5521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
5581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
5641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
5701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
5761 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
5821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
5881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
5941 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
6001 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gc
//
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