| 质粒名称: | pMaster1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 58525 |
| 存储实验室: | Mario Capecchi |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | 816 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMaster1 was a gift from Mario Capecchi (Addgene plasmid # 58525 ; http://n2t.net/addgene:58525 ; RRID:Addgene_58525) |
| 参考文献部分: | Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Wu S, Wu Y, Zhang X, Capecchi MR. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7. 10.1073/pnas.1409933111 PubMed 25002522 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMaster1 21149 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Reprogram somatic cells to iPS cells.
ACCESSION .
VERSION .
KEYWORDS pMaster1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 21149)
AUTHORS Wu S, Wu Y, Zhang X, Capecchi MR
TITLE Efficient germ-line transmission obtained with transgene-free
induced pluripotent stem cells.
JOURNAL Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi:
10.1073/pnas.1409933111. Epub 2014 Jul 7.
PUBMED 25002522
REFERENCE 2 (bases 1 to 21149)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..21149
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1978..3767
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
promoter 4243..5421
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 4474..5412
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 5369..5389
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 5429..5438
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6524..6589
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
CDS 8009..8062
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 9023..9082
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS 9083..10402
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
polyA_signal 10526..10647
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10563..10582)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10617..10636
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(10654..11109)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10791..10812)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 11003..11022
/label=F1ori-R
/note="F1 origin, reverse primer"
enhancer 11235..11538
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 11540..11817
/label=chicken beta-actin promoter
intron 11818..12834
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 12907..14037
/codon_start=1
/product="herpes simplex virus thymidine kinase"
/label=HSV TK
/note="confers sensitivity to nucleoside analogs such as
acyclovir or ganciclovir"
/translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
misc_feature 14053..14639
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(14220..14237)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 14447..14466
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 14640..15443
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(14703..14722)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 15313..15332
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(15631..15650)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 15675..15722
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
enhancer 15734..16113
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 16115..16392
/label=chicken beta-actin promoter
intron 16393..17409
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 18402..18467
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
rep_origin complement(19279..19867)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(19359..19378)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(20038..20898)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 20661..20680
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(20899..21003)
/gene="bla"
/label=AmpR promoter
primer_bind 21071..21089
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ggccgcaccc agcttttctt ccgttgcccc agtagcatct ctgtctggtg accttgaaga
61 ggaagaggag gggtcccgag aatccccatc cctaccgtcc agcaaaaagg gggacgagga
121 atttgaggcc tggcttgagg ctcaggacgc aaatcttgag gatgttcagc gggagttttc
181 cgggctgcga gtaattggtg atgaggacga ggatggttcg gaggatgggg aattttcaga
241 cctggatctg tctgacagcg accatgaagg ggatgagggt gggggggctg ttggaggggg
301 caggagtctg cactccctgt attcactgag cgtcgtctaa taaagatgtc tattgatctc
361 ttttagtgtg aatcatgtct gacgaggggc caggtacagg acctggaaat ggcctaggag
421 agaagggaga cacatctgga ccagaaggct ccggcggcag tggacctcaa agaagagggg
481 gtgataacca tggacgagga cggggaagag gacgaggacg aggaggcgga agaccaggag
541 ccccgggcgg ctcaggatca gggccaagac atagagatgg tgtccggaga ccccaaaaac
601 gtccaagttg cattggctgc aaagggaccc acggtggaac aggagcagga gcaggagcgg
661 gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag
721 caggaggtgg aggccggggt cgaggaggca gtggaggccg gggtcgagga ggtagtggag
781 gccggggtcg aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga gccagggggg
841 gaagtcgtga aagagccagg gggagaggtc gtggacgtgg agaaaagagg cccaggagtc
901 ccagtagtca gtcatcatca tccgggtctc caccgcgcag gccccctcca ggtagaaggc
961 catttttcca ccctgtaggg gaagccgatt attttgaata ccaccaagaa ggtggcccag
1021 atggtgagcc tgacgtgccc ccgggagcga tagagcaggg ccccgcagat gacccaggag
1081 aaggcccaag cactggaccc cggggtcagg gtgatggagg caggcgcaaa aaaggagggt
1141 ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa atttgagaac attgcagaag
1201 gtttaagagc tctcctggct aggagtcacg tagaaaggac taccgacgaa ggaacttggg
1261 tcgccggtgt gttcgtatat ggaggtagta agacctccct ttacaaccta aggcgaggaa
1321 ctgcccttgc tattccacaa tgtcgtctta caccattgag tcgtctcccc tttggaatgg
1381 cccctggacc cggcccacaa cctggcccgc taagggagtc cattgtctgt tatttcatgg
1441 tctttttaca aactcatata tttgctgagg ttttgaagga tgcgattaag gaccttgtta
1501 tgacaaagcc cgctcctacc tgcaatatca gggtgactgt gtgcagcttt gacgatggag
1561 tagatttgcc tccctggttt ccacctatgg tggaaggggc tgccgcggag ggtgatgacg
1621 gagatgacgg agatgaagga ggtgatggag atgagggtga ggaagggcag gagtgatgta
1681 acttgttagg agacgccctc aatcgtatta aaagccgtgt attcccccgc actaaagaat
1741 aaatccccag tagacatcat gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc
1801 attttcgtcc tcccaacatg gggcaattgg gcatacccat gttgtcacgt cactcagctc
1861 cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt gagcaatcag
1921 acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag caaccagcag
1981 gaaaaggaca agcagcgaaa attcacgccc ccttgggagg tggcggcata tgcaaaggat
2041 agcactccca ctctactact gggtatcata tgctgactgt atatgcatga ggatagcata
2101 tgctacccgg atacagatta ggatagcata tactacccag atatagatta ggatagcata
2161 tgctacccag atatagatta ggatagccta tgctacccag atataaatta ggatagcata
2221 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta
2281 tgctacccag atatagatta ggatagcata tgctacccag atatagatta ggatagcata
2341 tgctatccag atatttgggt agtatatgct acccagatat aaattaggat agcatatact
2401 accctaatct ctattaggat agcatatgct acccggatac agattaggat agcatatact
2461 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
2521 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct
2581 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct
2641 atccagatat ttgggtagta tatgctaccc atggcaacat tagcccaccg tgctctcagc
2701 gacctcgtga atatgaggac caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta
2761 atttgtcctc cagatcgcag caatcgcgcc cctatcttgg cccgcccacc tacttatgca
2821 ggtattcccc ggggtgccat tagtggtttt gtgggcaagt ggtttgaccg cagtggttag
2881 cggggttaca atcagccaag ttattacacc cttattttac agtccaaaac cgcagggcgg
2941 cgtgtggggg ctgacgcgtg cccccactcc acaatttcaa aaaaaagagt ggccacttgt
3001 ctttgtttat gggccccatt ggcgtggagc cccgtttaat tttcgggggt gttagagaca
3061 accagtggag tccgctgctg tcggcgtcca ctctctttcc ccttgttaca aatagagtgt
3121 aacaacatgg ttcacctgtc ttggtccctg cctgggacac atcttaataa ccccagtatc
3181 atattgcact aggattatgt gttgcccata gccataaatt cgtgtgagat ggacatccag
3241 tctttacggc ttgtccccac cccatggatt tctattgtta aagatattca gaatgtttca
3301 ttcctacact agtatttatt gcccaagggg tttgtgaggg ttatattggt gtcatagcac
3361 aatgccacca ctgaaccccc cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt
3421 tttcgagcac ctcacataca ccttactgtt cacaactcag cagttattct attagctaaa
3481 cgaaggagaa tgaagaagca ggcgaagatt caggagagtt cactgcccgc tccttgatct
3541 tcagccactg cccttgtgac taaaatggtt cactaccctc gtggaatcct gaccccatgt
3601 aaataaaacc gtgacagctc atggggtggg agatatcgct gttccttagg acccttttac
3661 taaccctaat tcgatagcat atgcttcccg ttgggtaaca tatgctattg aattagggtt
3721 agtctggata gtatatacta ctacccggga agcatatgct acccgtttag ggttaacaag
3781 ggggccttat aaacactatt gctaatgccc tcttgagggt ccgcttatcg gtagctacac
3841 aggcccctct gattgacgtt ggtgtagcct cccgtagtct tcctgggccc ctgggaggta
3901 catgtccccc agcattggtg taagagcttc agccaagagt tacacataaa ggcaatgttg
3961 tgttgcagtc cacagactgc aaagtctgct ccaggatgaa agccactcag tgttggcaaa
4021 tgtgcacatc catttataag gatgtcaact acagtcagag aacccctttg tgtttggtcc
4081 ccccccgtgt cacatgtgga acagggccca gttggcaagt tgtaccaacc aactgaaggg
4141 attacatgca ctgccccgcg aagaaggggc agagatgtcg tagtcaggtt tagttcgtcc
4201 ggggcgggga tcgatcctct agagtcgatt aattaatcgt gaggctccgg tgcccgtcag
4261 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
4321 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
4381 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
4441 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
4501 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
4561 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
4621 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
4681 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
4741 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
4801 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
4861 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
4921 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
4981 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
5041 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
5101 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
5161 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
5221 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
5281 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
5341 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
5401 cttccatttc aggtgtcgtg aagctagcgc caccatggcg ggacacctgg cttcggattt
5461 cgccttctcg ccccctccag gtggtggagg tgatgggcca ggggggccgg agccgggctg
5521 ggttgatcct cggacctggc taagcttcca aggccctcct ggagggccag gaatcgggcc
5581 gggggttggg ccaggctctg aggtgtgggg gattccccca tgccccccgc cgtatgagtt
5641 ctgtgggggg atggcgtact gtgggcccca ggttggagtg gggctagtgc cccaaggcgg
5701 cttggagacc tctcagcctg agggcgaagc aggagtcggg gtggagagca actccgatgg
5761 ggcctccccg gagccctgca ccgtcacccc tggtgccgtg aagctggaga aggagaagct
5821 ggagcaaaac ccggaggagt cccaggacat caaagctctg cagaaagaac tcgagcaatt
5881 tgccaagctc ctgaagcaga agaggatcac cctgggatat acacaggccg atgtggggct
5941 caccctgggg gttctatttg ggaaggtatt cagccaaacg accatctgcc gctttgaggc
6001 tctgcagctt agcttcaaga acatgtgtaa gctgcggccc ttgctgcaga agtgggtgga
6061 ggaagctgac aacaatgaaa atcttcagga gatatgcaaa gcagaaaccc tcgtgcaggc
6121 ccgaaagaga aagcgaacca gtatcgagaa ccgagtgaga ggcaacctgg agaatttgtt
6181 cctgcagtgc ccgaaaccca cactgcagca gatcagccac atcgcccagc agcttgggct
6241 cgagaaggat gtggtccgag tgtggttctg taaccggcgc cagaagggca agcgatcaag
6301 cagcgactat gcacaacgag aggattttga ggctgctggg tctcctttct cagggggacc
6361 agtgtccttt cctctggccc cagggcccca ttttggtacc ccaggctatg ggagccctca
6421 cttcactgca ctgtactcct cggtcccttt ccctgagggg gaagcctttc cccctgtctc
6481 cgtcaccact ctgggctctc ccatgcattc aaacggaagt ggcgtgaaac agactttgaa
6541 ttttgacctt ctcaagttgg cgggagacgt ggagtccaac ccagggccca tggctgtcag
6601 cgacgcgctg ctcccatctt tctccacgtt cgcgtctggc ccggcgggaa gggagaagac
6661 actgcgtcaa gcaggtgccc cgaataaccg ctggcgggag gagctctccc acatgaagcg
6721 acttccccca gtgcttcccg gccgccccta tgacctggcg gcggcgaccg tggccacaga
6781 cctggagagc ggcggagccg gtgcggcttg cggcggtagc aacctggcgc ccctacctcg
6841 gagagagacc gaggagttca acgatctcct ggacctggac tttattctct ccaattcgct
6901 gacccatcct ccggagtcag tggccgccac cgtgtcctcg tcagcgtcag cctcctcttc
6961 gtcgtcgccg tcgagcagcg gccctgccag cgcgccctcc acctgcagct tcacctatcc
7021 gatccgggcc gggaacgacc cgggcgtggc gccgggcggc acgggcggag gcctcctcta
7081 tggcagggag tccgctcccc ctccgacggc tcccttcaac ctggcggaca tcaacgacgt
7141 gagcccctcg ggcggcttcg tggccgagct cctgcggcca gaattggacc cggtgtacat
7201 tccgccgcag cagccgcagc cgccaggtgg cgggctgatg ggcaagttcg tgctgaaggc
7261 gtcgctgagc gcccctggca gcgagtacgg cagcccgtcg gtcatcagcg tcagcaaagg
7321 cagccctgac ggcagccacc cggtggtggt ggcgccctac aacggcgggc cgccgcgcac
7381 gtgccccaag atcaagcagg aggcggtctc ttcgtgcacc cacttgggcg ctggaccccc
7441 tctcagcaat ggccaccggc cggctgcaca cgacttcccc ctggggcggc agctccccag
7501 caggactacc ccgaccctgg gtcttgagga agtgctgagc agcagggact gtcaccctgc
7561 cctgccgctt cctcccggct tccatcccca cccggggccc aattacccat ccttcctgcc
7621 cgatcagatg cagccgcaag tcccgccgct ccattaccaa gagctcatgc cacccggttc
7681 ctgcatgcca gaggagccca agccaaagag gggaagacga tcgtggcccc ggaaaaggac
7741 cgccacccac acttgtgatt acgcgggctg cggcaaaacc tacacaaaga gttcccatct
7801 caaggcacac ctgcgaaccc acacaggtga gaaaccttac cactgtgact gggacggctg
7861 tggatggaaa ttcgcccgct cagatgaact gaccaggcac taccgtaaac acacggggca
7921 ccgcccgttc cagtgccaaa aatgcgaccg agcattttcc aggtcggacc acctcgcctt
7981 acacatgaag aggcattttg gcagcggcga gggcaggggc agcctgctga cctgcggcga
8041 cgtggaggag aaccccggcc ccatgtacaa catgatggag acggagctga agccgccggg
8101 cccgcagcaa acttcggggg gcggcggcgg caactccacc gcggcggcgg ccggcggcaa
8161 ccagaaaaac agcccggacc gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg
8221 cgggcagcgg cgcaagatgg cccaggagaa ccccaagatg cacaactcgg agatcagcaa
8281 gcgcctgggc gccgagtgga aacttttgtc ggagacggag aagcggccgt tcatcgacga
8341 ggctaagcgg ctgcgagcgc tgcacatgaa ggagcacccg gattataaat accggccccg
8401 gcggaaaacc aagacgctca tgaagaagga taagtacacg ctgcccggcg ggctgctggc
8461 ccccggcggc aatagcatgg cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt
8521 gaaccagcgc atggacagtt acgcgcacat gaacggctgg agcaacggca gctacagcat
8581 gatgcaggac cagctgggct acccgcagca cccgggcctc aatgcgcacg gcgcagcgca
8641 gatgcagccc atgcaccgct acgacgtgag cgccctgcag tacaactcca tgaccagctc
8701 gcagacctac atgaacggct cgcccaccta cagcatgtcc tactcgcagc agggcacccc
8761 tggcatggct cttggctcca tgggttcggt ggtcaagtcc gaggccagct ccagcccccc
8821 tgtggttacc tcttcctccc actccagggc gccctgccag gccggggacc tccgggacat
8881 gatcagcatg tatctccccg gcgccgaggt gccggaaccc gccgccccca gcagacttca
8941 catgtcccag cactaccaga gcggcccggt gcccggcacg gccattaacg gcacactgcc
9001 cctctcacac atgggtagtg ggcaatgtac taactacgct ttgttgaaac tcgctggcga
9061 tgttgaaagt aaccccggtc ctatgcccct caacgttagc ttcaccaaca ggaactatga
9121 cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga acttctacca
9181 gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct ggaagaaatt
9241 cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct gctcgccctc
9301 ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg gcgggagctt
9361 ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca tggtgaacca
9421 gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca tccaggactg
9481 tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg cctcctacca
9541 ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg tctgctccac
9601 ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg acccctcggt
9661 ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct cgcaagactc
9721 cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct ccccgcaggg
9781 cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca gcgactctga
9841 ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga ggcaggctcc
9901 tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac ctcctcacag
9961 cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg cagcgcctcc
10021 ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg tcagagtcct
10081 gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca ccgaggagaa
10141 tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag
10201 cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt
10261 agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct
10321 catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca
10381 gctacggaac tcttgtgcgt aatagcggcc gcgactctag atcataatca gccataccac
10441 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
10501 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
10561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
10621 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
10681 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
10741 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
10801 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
10861 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
10921 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
10981 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
11041 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
11101 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
11161 tctaaataca gcgctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
11221 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
11281 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
11341 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
11401 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
11461 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
11521 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
11581 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
11641 ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
11701 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
11761 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
11821 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
11881 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
11941 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
12001 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
12061 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
12121 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
12181 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
12241 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
12301 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
12361 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
12421 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
12481 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
12541 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
12601 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
12661 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
12721 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
12781 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
12841 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcta gggataacag
12901 ggtaatatgg cttcgtaccc cggccatcaa cacgcgtctg cgttcgacca ggctgcgcgt
12961 tctcgcggcc atagcaaccg acgtacggcg ttgcgccctc gccggcagca agaagccacg
13021 gaagtccgcc cggagcagaa aatgcccacg ctactgcggg tttatataga cggtccccac
13081 gggatgggga aaaccaccac cacgcaactg ctggtggccc tgggttcgcg cgacgatatc
13141 gtctacgtac ccgagccgat gacttactgg cgggtgctgg gggcttccga gacaatcgcg
13201 aacatctaca ccacacaaca ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg
13261 gtggtaatga caagcgccca gataacaatg ggcatgcctt atgccgtgac cgacgccgtt
13321 ctggctcctc atatcggggg ggaggctggg agctcacatg ccccgccccc ggccctcacc
13381 ctcatcttcg accgccatcc catcgccgcc ctcctgtgct acccggccgc gcggtacctt
13441 atgggcagca tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg
13501 cccggcacca acatcgtgct tggggccctt ccggaggaca gacacatcga ccgcctggcc
13561 aaacgccagc gccccggcga gcggctggac ctggctatgc tggctgcgat tcgccgcgtt
13621 tacgggctac ttgccaatac ggtgcggtat ctgcagtgcg gcgggtcgtg gcgggaggac
13681 tggggacagc tttcggggac ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg
13741 ggcccacgac cccatatcgg ggacacgtta tttaccctgt ttcgggcccc cgagttgctg
13801 gcccccaacg gcgacctgta taacgtgttt gcctgggcct tggacgtctt ggccaaacgc
13861 ctccgttcca tgcacgtctt tatcctggat tacgaccaat cgcccgccgg ctgccgggac
13921 gccctgctgc aacttacctc cgggatggtc cagacccacg tcaccacccc cggctccata
13981 ccgacgatat gcgacctggc gcgcacgttt gcccgggaga tgggggaggc taactgagcg
14041 gccgcggatc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
14101 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
14161 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
14221 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
14281 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
14341 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
14401 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
14461 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
14521 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
14581 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca
14641 tgggatccgc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
14701 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
14761 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
14821 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
14881 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
14941 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
15001 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
15061 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
15121 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
15181 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
15241 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
15301 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
15361 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
15421 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
15481 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
15541 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
15601 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
15661 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
15721 tcggcgcgcc ctcgacattg attattgact agttattaat agtaatcaat tacggggtca
15781 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
15841 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
15901 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
15961 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
16021 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
16081 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
16141 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
16201 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
16261 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
16321 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
16381 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
16441 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
16501 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
16561 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
16621 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
16681 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
16741 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
16801 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
16861 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
16921 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
16981 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
17041 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
17101 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
17161 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
17221 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
17281 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
17341 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
17401 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
17461 ttcgcgatta agccaccatg agtgtggatc cagcttgtcc ccaaagcttg ccttgctttg
17521 aagcatccga ctgtaaagaa tcttcaccta tgcctgtgat ttgtgggcct gaagaaaact
17581 atccatcctt gcaaatgtct tctgctgaga tgcctcacac ggagactgtc tctcctcttc
17641 cttcctccat ggatctgctt attcaggaca gccctgattc ttccaccagt cccaaaggca
17701 aacaacccac ttctgcagag aagagtgtcg caaaaaagga agacaaggtc ccggtcaaga
17761 aacagaagac cagaactgtg ttctcttcca cccagctgtg tgtactcaat gatagatttc
17821 agagacagaa atacctcagc ctccagcaga tgcaagaact ctccaacatc ctgaacctca
17881 gctacaaaca ggtgaagacc tggttccaga accagagaat gaaatctaag aggtggcaga
17941 aaaacaactg gccgaagaat agcaatggtg tgacgcagaa ggcctcagca cctacctacc
18001 ccagccttta ctcttcctac caccagggat gcctggtgaa cccgactggg aaccttccaa
18061 tgtggagcaa ccagacctgg aacaattcaa cctggagcaa ccagacccag aacatccagt
18121 cctggagcaa ccactcctgg aacactcaga cctggtgcac ccaatcctgg aacaatcagg
18181 cctggaacag tcccttctat aactgtggag aggaatctct gcagtcctgc atgcagttcc
18241 agccaaattc tcctgccagt gacttggagg ctgccttgga agctgctggg gaaggcctta
18301 atgtaataca gcagaccact aggtatttta gtactccaca aaccatggat ttattcctaa
18361 actactccat gaacatgcaa cctgaagacg tgggaagtgg cgtgaaacag actttgaatt
18421 ttgaccttct caagttggcg ggagacgtgg agtccaaccc agggcccatg ggctccgtgt
18481 ccaaccagca gtttgcaggt ggctgcgcca aggcggcaga agaggcgccc gaggaggcgc
18541 cggaggacgc ggcccgggcg gcggacgagc ctcagctgct gcacggtgcg ggcatctgta
18601 agtggttcaa cgtgcgcatg gggttcggct tcctgtccat gaccgcccgc gccggggtcg
18661 cgctcgaccc cccagtggat gtctttgtgc accagagtaa gctgcacatg gaagggttcc
18721 ggagcttgaa ggagggtgag gcagtggagt tcacctttaa gaagtcagcc aagggtctgg
18781 aatccatccg tgtcaccgga cctggtggag tattctgtat tgggagtgag aggcggccaa
18841 aaggaaagag catgcagaag cgcagatcaa aaggagacag gtgctacaac tgtggaggtc
18901 tagatcatca tgccaaggaa tgcaagctgc caccccagcc caagaagtgc cacttctgcc
18961 agagcatcag ccatatggta gcctcatgtc cgctgaaggc ccagcagggc cctagtgcac
19021 agggaaagcc aacctacttt cgagaggaag aagaagaaat ccacagccct accctgctcc
19081 cggaggcaca gaattgataa gataaacgtc gacgcgaggc tggatggcct tccccattat
19141 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
19201 ggtagatgac gaccatcagg gacagcttca aggccagcaa aaggccagga accgtaaaaa
19261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
19321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
19381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
19441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
19501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
19561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
19621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
19681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
19741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
19801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
19861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
19921 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
19981 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
20041 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
20101 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
20161 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
20221 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
20281 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
20341 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
20401 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
20461 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
20521 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
20581 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
20641 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
20701 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
20761 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
20821 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
20881 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
20941 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
21001 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
21061 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattct catgtttgac
21121 agcttatcat cgataagctg atcctcaca
//
