pGL4.84[hRlucCP/Puro]

价格:1500元

联系方式:I47-825O-882O

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pGL4.84载体基本信息

出品公司: Promega
载体名称: pGL4.84 ; pGL4.84[hRlucCP/Puro]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 4866 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRlucCP
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.84[hRlucCP/Puro]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRlucCP。
产品目录号: E7521
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.84载体质粒图谱和多克隆位点信息

pGL4.84载体图谱



pGL4.84 多克隆位点

pGL4.84 载体特征

pGL4.84载体简介

pGL4.84载体简介
The pGL4.84[hRlucCP/Puro] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for puromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. It has also been engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.84[hRlucCP/Puro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

pGL4.84载体序列

LOCUS       DQ188848                4866 bp    DNA     circular SYN 16-NOV-2005
DEFINITION  Synthetic Renilla luciferase reporter vector pGL4.84[hRlucCP/Puro],
            complete sequence.
ACCESSION   DQ188848
VERSION     DQ188848.1  GI:76364322
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.84[hRlucCP/Puro]
  ORGANISM  Renilla luciferase reporter vector pGL4.84[hRlucCP/Puro]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Renilla luciferase reporter vector
                     pGL4.84[hRlucCP/Puro]"
                     /mol_type="other DNA"
                     /db_xref="taxon:346095"
     misc_feature    1..70
                     /note="multiple cloning region"
     CDS             100..1212
                     /note="synthetic Renilla luciferase; contains hPEST and
                     hCL1 destabilization sequences"
                     /codon_start=1
                     /product="hRlucCP"
                     /protein_id="ABA41686.1"
                     /db_xref="GI:76364323"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF
                     PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     regulatory      1249..1470
                     /regulatory_class="other"
                     /note="SV40 late poly(A) signal"
     regulatory      1518..1936
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter"
     CDS             1961..2560
                     /note="puromycin resistance"
                     /codon_start=1
                     /product="puromycin-N-acetyltransferase"
                     /protein_id="ABA41687.1"
                     /db_xref="GI:76364324"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     regulatory      2585..2633
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal"
     primer_bind     2700..2719
                     /note="Reporter Vector primer 4 (RVprimer4) binding
                     region"
     rep_origin      2957
                     /note="ColEI-derived plasmid replication origin"
     gene            complement(3748..4608)
                     /gene="bla"
     CDS             complement(3748..4608)
                     /gene="bla"
                     /note="ampicillin resistance"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="ABA41688.1"
                     /db_xref="GI:76364325"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4713..4866
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal/transcriptional pause
                     site"
     primer_bind     4815..4834
                     /note="Reporter Vector primer 3 (RVprimer3) binding
                     region"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
     1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
     1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
     1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
     1501 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     1561 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1921 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgaccgagt acaagcctac
     1981 cgtgcgcctg gccactcgcg atgatgtgcc ccgcgccgtc cgcactctgg ccgccgcttt
     2041 cgccgactac cccgctaccc ggcacaccgt ggaccccgac cggcacatcg agcgtgtgac
     2101 agagttgcag gagctgttcc tgacccgcgt cgggctggac atcggcaagg tgtgggtagc
     2161 cgacgacggc gcggccgtgg ccgtgtggac tacccccgag agcgttgagg ccggcgccgt
     2221 gttcgccgag atcggccccc gaatggccga gctgagcggc agccgcctgg ccgcccagca
     2281 gcaaatggag ggcctgcttg ccccccatcg tcccaaggag cctgcctggt ttctggccac
     2341 tgtaggagtg agccccgacc accagggcaa gggcttgggc agcgccgtcg tgttgcccgg
     2401 cgtagaggcc gccgaacgcg ccggtgtgcc cgcctttctc gaaacaagcg caccaagaaa
     2461 ccttccattc tacgagcgcc tgggcttcac cgtgaccgcc gatgtcgagg tgcccgaggg
     2521 acctaggacc tggtgtatga cacgaaaacc tggcgcctaa tgatctagaa ccggtcatgg
     2581 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac
     2641 tagatgctgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     2701 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     2761 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     3721 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     3781 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     3841 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     3901 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     3961 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     4021 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     4081 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     4141 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     4201 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     4261 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     4321 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     4381 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     4441 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     4501 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     4561 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     4621 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     4681 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     4741 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     4801 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     4861 ttctct
//

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