pAAV-AscI-CAG-Igk-NeIle-TM

LOCUS       pAAV-AscI-CAG-Igk-NeIle-TM               6817 bp DNA     circular SYN 15-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Vax
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2661..2691
                     /label=AscI-OxE insert
                     /label=AscI-OxE\insert
                     /note="/vntifkey=21"
     misc_feature    2692..2935
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="/vntifkey=21"
     promoter        2942..3219
                     /label=chicken beta-actin promoter
     sig_peptide     3646..3708
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             3715..5499
                     /codon_start=1
                     /label=NeIle
                     /note="/vntifkey=21"
                     /translation="MEDIKVAVVGAMSAGADWRISKEACPNGKTLISTLKWSYTTGDGK
                     RYRSTARTTFTFAKPMAANYLKNQPMYVFRRTELKHSKTEMDFKEWQKAFTDVVGMDEL
                     YKVDGGSGGTGMVSEGEVDNMASLPATHELHIFGSINGVGFDMVGQGSGNPNVGYEELN
                     LKSTKGELQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTVRFEDGAS
                     LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAFHIGPVTQYGDQEFTGAEQAV
                     ADINARGGIKGNKLQIVKYDDACDPKQAVAVANRVVNDGIKYVIGHLCSSSAQPASDIY
                     EDAGILMITPAATAPELTARGYQLILRTTGLDSDQGLTAAKYILEKVKPQRIAIVHDKQ
                     QYGEGLARAVQGGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMG
                     QILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKHIVDAVK
                     AKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLK
                     GFEFGVFDWHANGTATDAK"
     CDS             5518..5547
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5551..5697
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    5719..6307
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    6350..6471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6817,1..940)
                     /label=ColE1
                     /note="ColE1"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
     2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     3241 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     3301 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     3361 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     3421 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
     3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     3601 gctgtctcat cattttggca aagaattgga tccaccggtg acaccatgga gacagacaca
     3661 ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacag atctatggaa
     3721 gatattaaag tcgcggtcgt gggcgcaatg tccgctggtg ctgactggcg tatatccaag
     3781 gaggcttgcc ccaatggtaa gactcttatt tccacactta agtggtctta taccaccggc
     3841 gacggcaaac gctaccggtc tactgctcgc accacattca cattcgccaa accaatggcc
     3901 gcaaattacc tcaaaaatca gccgatgtat gtcttccgga gaacagagct gaaacattct
     3961 aagaccgaaa tggattttaa agagtggcaa aaggccttta cggacgtggt gggcatggac
     4021 gagctgtaca aggtggatgg cggcagcggt ggcaccggca tggtctcaga gggagaggtg
     4081 gacaacatgg ctagtctgcc tgctacccac gaactgcata tttttggctc cataaatggc
     4141 gtcggtttcg acatggtagg ccaaggctcc ggtaatccaa acgtcggtta tgaggagctg
     4201 aacctgaagt ccaccaaggg cgaactgcag ttcagtccct ggatcctggt gccgcacatt
     4261 gggtatgggt ttcatcagta tcttccctac ccggacggga tgtcgccatt tcaggctgca
     4321 atggtcgacg ggtccggata tcaggtgcac cgcactgtgc ggtttgagga cggtgcttca
     4381 ctgaccgtga attacagata cacctacgaa ggatcacata tcaaagggga agctcaggtg
     4441 aagggcactg ggttcccggc agacggccct gtgatgacaa attccctgac agcctttcat
     4501 attggtccgg tgacccagta cggtgaccag gagtttaccg gcgcagagca ggcggttgcg
     4561 gatatcaacg ctagaggcgg cattaagggc aacaaactgc aaatcgtaaa atatgacgat
     4621 gcctgtgacc cgaaacaggc tgttgcggtg gcgaacagag tcgttaacga cggcattaaa
     4681 tatgtgattg gtcacctctg ttcttcatca gcgcagcctg cgtctgacat ctacgaagac
     4741 gcaggcattt taatgatcac cccagcggca accgcgccgg agctgaccgc ccgtggctat
     4801 cagctgatcc tgcgcaccac cggcctggac tccgaccagg ggctgacggc ggcgaaatat
     4861 attcttgaga aagtgaaacc gcagcgtatt gctatcgttc acgacaaaca gcagtacggc
     4921 gaaggtctgg cgcgagcggt gcagggtggc ctgaagaaag gcaatgcaaa cgtggtgttc
     4981 tttgatggca tcaccgccgg ggaaaaagat ttctcaacgc tggtggcgcg tctgaaaaaa
     5041 gagaatatcg acttcgttta ctacggcggt tatcacccgg aaatggggca aatcctgcgt
     5101 caggcacgcg cggcagggct gaaaactcag tttatggggc cggagggtgt ggctaacgtt
     5161 tcgctgtcta acattgcggg cgaatcagcg gaagggctgc tggtgaccaa gccgaagaac
     5221 tacgatcagg ttccggcgaa caaacacatt gttgacgcag tcaaagcgaa aaaacaggac
     5281 ccaagtggcg cattcgtttg gaccacctac gccgcgctgc aatctttgca ggcgggcctg
     5341 aatcagtctg acgatccggc tgaaatcgcc aaatacctga aagcgaactc cgtggatacc
     5401 gtaatgggac cgctgacctg ggatgagaaa ggcgatctga aaggctttga gttcggcgta
     5461 tttgactggc acgccaacgg cacggcgacc gatgcgaaga ctagtctgca ggtcgacgaa
     5521 caaaaactca tctcagaaga ggatctgaat gctgtgggcc aggacacgca ggaggtcatc
     5581 gtggtgccac actccttgcc ctttaaggtg gtggtgatct cagccatcct ggccctggtg
     5641 gtgctcacca tcatctccct tatcatcctc atcatgcttt ggcagaagaa gccacgttag
     5701 gaattcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5761 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5821 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5881 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5941 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6001 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6061 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6121 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6181 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6241 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6301 tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt
     6361 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     6421 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     6481 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
     6541 taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga
     6601 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     6661 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     6721 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc
     6781 caacgcgcgg ggagaggcgg tttgcgtatt gggcgct
//