| 质粒名称: | pT3-EF1a-c-Met |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31784 |
| 存储实验室: | Xin Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pT3-EF1a |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Met |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4173 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EF1alpha |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | EF-1a-Fwd |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pT3-EF1a-c-Met was a gift from Xin Chen (Addgene plasmid # 31784 ; http://n2t.net/addgene:31784 ; RRID:Addgene_31784) |
| 参考文献部分: | Distinct pathways of genomic progression to benign and malignant tumors of the liver. Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang R, Bishop JM. Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep 4. 10.1073/pnas.0706578104 PubMed 17785413 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pT3-EF1a-c-Met 9743 bp ds-DNA circular SYN 19-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pT3-EF1a-c-Met
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9743)
AUTHORS Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang
R, Bishop JM
TITLE Distinct pathways of genomic progression to benign and malignant
tumors of the liver.
JOURNAL Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep
4.
PUBMED 17785413
REFERENCE 2 (bases 1 to 9743)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9743
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 174..193
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 427..444
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 561..582
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 597..627
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 635..651
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 640..662
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 659..675
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 659..675
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1101..1120)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind complement(1173..1206)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter 1347..2525
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1578..2516
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 2473..2493
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 2542..2561
/label=T7
/note="T7 promoter, forward primer"
promoter 2542..2560
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 2639..2663
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(6855..6879)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 6932..6973
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
primer_bind complement(7011..7028)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7017..7241
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind complement(7311..7344)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 7400..7420
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
primer_bind complement(7802..7819)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7802..7818)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7811..7833)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(8027..8046)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8146..8168
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(8206..8224)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8292..8396
/gene="bla"
/label=AmpR promoter
CDS 8397..9257
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8615..8634)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9428..273
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
61 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
121 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
181 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
241 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
301 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
361 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
421 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
481 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
541 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
601 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
661 ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagacct
721 atacagttga agtcggaagt ttacatacac ttaagttgga gtcattaaaa ctcgtttttc
781 aactactcca caaatttctt gttaacaaac aatagttttg gcaagtcagt taggacatct
841 actttgtgca tgacacaagt catttttcca acaattgttt acagacagat tatttcactt
901 ataattcact gtatcacaat tccagtgggt cagaagttta catacactaa gttgactgtg
961 cctttaaaca gcttggaaaa ttccagaaaa tgatgtcatg gctttagaag cttctgatag
1021 actaattgac atcatttgag tcaattggag gtgtacctgt ggatgtattt caaggggatc
1081 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagcggccgc
1141 tagctcgaga tcttaattaa actagtatcg atataacttc gtataatgta tgctatacga
1201 agttatatgt acgggccaga tatacgcgtt gacattgatt attgactagg cttttgcaaa
1261 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
1321 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
1381 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
1441 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
1501 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
1561 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
1621 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
1681 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
1741 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
1801 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
1861 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
1921 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
1981 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
2041 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
2101 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
2161 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
2221 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
2281 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
2341 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
2401 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
2461 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
2521 cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt
2581 aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatcaac
2641 aagtttgtac aaaaaagcag gcttcaaaga taaacctctc ataatgaagg cccccgctgt
2701 gcttgcacct ggcatcctcg tgctcctgtt taccttggtg cagaggagca atggggagtg
2761 taaagaggca ctagcaaagt ccgagatgaa tgtgaatatg aagtatcagc ttcccaactt
2821 caccgcggaa acacccatcc agaatgtcat tctacatgag catcacattt tccttggtgc
2881 cactaactac atttatgttt taaatgagga agaccttcag aaggttgctg agtacaagac
2941 tgggcctgtg ctggaacacc cagattgttt cccatgtcag gactgcagca gcaaagccaa
3001 tttatcagga ggtgtttgga aagataacat caacatggct ctagttgtcg acacctacta
3061 tgatgatcaa ctcattagct gtggcagcgt caacagaggg acctgccagc gacatgtctt
3121 tccccacaat catactgctg acatacagtc ggaggttcac tgcatattct ccccacagat
3181 agaagagccc agccagtgtc ctgactgtgt ggtgagcgcc ctgggagcca aagtcctttc
3241 atctgtaaag gaccggttca tcaacttctt tgtaggcaat accataaatt cttcttattt
3301 cccagatcat ccattgcatt cgatatcagt gagaaggcta aaggaaacga aagatggttt
3361 tatgtttttg acggaccagt cctacattga tgttttacct gagttcagag attcttaccc
3421 cattaagtat gtccatgcct ttgaaagcaa caattttatt tacttcttga cggtccaaag
3481 ggaaactcta gatgctcaga cttttcacac aagaataatc aggttctgtt ccataaactc
3541 tggattgcat tcctacatgg aaatgcctct ggagtgtatt ctcacagaaa agagaaaaaa
3601 gagatccaca aagaaggaag tgtttaatat acttcaggct gcgtatgtca gcaagcctgg
3661 ggcccagctt gctagacaaa taggagccag cctgaatgat gacattcttt tcggggtgtt
3721 cgcacaaagc aagccagatt ctgccgaacc aatggatcga tctgccatgt gtgcattccc
3781 tatcaaatat gtcaacgact tcttcaacaa gatcgtcaac aaaaacaatg tgagatgtct
3841 ccagcatttt tacggaccca atcatgagca ctgctttaat aggacacttc tgagaaattc
3901 atcaggctgt gaagcgcgcc gtgatgaata tcgaacagag tttaccacag ctttgcagcg
3961 cgttgactta ttcatgggtc aattcagcga agtcctctta acatctatat ccaccttcat
4021 taaaggagac ctcaccatag ctaatcttgg gacatcagag ggtcgcttca tgcaggttgt
4081 ggtttctcga tcaggaccat caacccctca tgtgaatttt ctcctggact cccatccagt
4141 gtctccagaa gtgattgtgg agcatacatt aaaccaaaat ggctacacac tggttatcac
4201 tgggaagaag atcacgaaga tcccattgaa tggcttgggc tgcagacatt tccagtcctg
4261 cagtcaatgc ctctctgccc caccctttgt tcagtgtggc tggtgccacg acaaatgtgt
4321 gcgatcggag gaatgcctga gcgggacatg gactcaacag atctgtctgc ctgcaatcta
4381 caaggttttc ccaaatagtg caccccttga aggagggaca aggctgacca tatgtggctg
4441 ggactttgga tttcggagga ataataaatt tgatttaaag aaaactagag ttctccttgg
4501 aaatgagagc tgcaccttga ctttaagtga gagcacgatg aatacattga aatgcacagt
4561 tggtcctgcc atgaataagc atttcaatat gtccataatt atttcaaatg gccacgggac
4621 aacacaatac agtacattct cctatgtgga tcctgtaata acaagtattt cgccgaaata
4681 cggtcctatg gctggtggca ctttacttac tttaactgga aattacctaa acagtgggaa
4741 ttctagacac atttcaattg gtggaaaaac atgtacttta aaaagtgtgt caaacagtat
4801 tcttgaatgt tataccccag cccaaaccat ttcaactgag tttgctgtta aattgaaaat
4861 tgacttagcc aaccgagaga caagcatctt cagttaccgt gaagatccca ttgtctatga
4921 aattcatcca accaaatctt ttattagtgg tgggagcaca ataacaggtg ttgggaaaaa
4981 cctgaattca gttagtgtcc cgagaatggt cataaatgtg catgaagcag gaaggaactt
5041 tacagtggca tgtcaacatc gctctaattc agagataatc tgttgtacca ctccttccct
5101 gcaacagctg aatctgcaac tccccctgaa aaccaaagcc tttttcatgt tagatgggat
5161 cctttccaaa tactttgatc tcatttatgt acataatcct gtgtttaagc cttttgaaaa
5221 gccagtgatg atctcaatgg gcaatgaaaa tgtactggaa attaagggaa atgatattga
5281 ccctgaagca gttaaaggtg aagtgttaaa agttggaaat aagagctgtg agaatataca
5341 cttacattct gaagccgttt tatgcacggt ccccaatgac ctgctgaaat tgaacagcga
5401 gctaaatata gagtggaagc aagcaatttc ttcaaccgtc cttggaaaag taatagttca
5461 accagatcag aatttcacag gattgattgc tggtgttgtc tcaatatcaa cagcactgtt
5521 attactactt gggtttttcc tgtggctgaa aaagagaaag caaattaaag atctgggcag
5581 tgaattagtt cgctacgatg caagagtaca cactcctcat ttggataggc ttgtaagtgc
5641 ccgaagtgta agcccaacta cagaaatggt ttcaaatgaa tctgtagact accgagctac
5701 ttttccagaa gatcagtttc ctaattcatc tcagaacggt tcatgccgac aagtgcagta
5761 tcctctgaca gacatgtccc ccatcctaac tagtggggac tctgatatat ccagtccatt
5821 actgcaaaat actgtccaca ttgacctcag tgctctaaat ccagagctgg tccaggcagt
5881 gcagcatgta gtgattgggc ccagtagcct gattgtgcat ttcaatgaag tcataggaag
5941 agggcatttt ggttgtgtat atcatgggac tttgttggac aatgatggca agaaaattca
6001 ctgtgctgtg aaatccttga acagaatcac tgacatagga gaagtttccc aatttctgac
6061 cgagggaatc atcatgaaag attttagtca tcccaatgtc ctctcgctcc tgggaatctg
6121 cctgcgaagt gaagggtctc cgctggtggt cctaccatac atgaaacatg gagatcttcg
6181 aaatttcatt cgaaatgaga ctcataatcc aactgtaaaa gatcttattg gctttggtct
6241 tcaagtagcc aaaggcatga aatatcttgc aagcaaaaag tttgtccaca gagacttggc
6301 tgcaagaaac tgtatgctgg atgaaaaatt cacagtcaag gttgctgatt ttggtcttgc
6361 cagagacatg tatgataaag aatactatag tgtacacaac aaaacaggtg caaagctgcc
6421 agtgaagtgg atggctttgg aaagtctgca aactcaaaag tttaccacca agtcagatgt
6481 gtggtccttt ggcgtgctcc tctgggagct gatgacaaga ggagccccac cttatcctga
6541 cgtaaacacc tttgatataa ctgtttactt gttgcaaggg agaagactcc tacaacccga
6601 atactgccca gaccccttat atgaagtaat gctaaaatgc tggcacccta aagccgaaat
6661 gcgcccatcc ttttctgaac tggtgtcccg gatatcagcg atcttctcta ctttcattgg
6721 ggagcactat gtccatgtga acgctactta tgtgaacgta aaatgtgtcg ctccgtatcc
6781 ttctctgttg tcatcagaag ataacgctga tgatgaggtg gacacacgac cagcctcctt
6841 ctgggagaca tcagacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
6901 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
6961 tctcgattct acgcgtaccg gttagtaatg agtttaaacc cgctgatcag cctcgactgt
7021 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
7081 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
7141 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
7201 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
7261 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa ataacttcgt
7321 ataatgtatg ctatacgaag ttatatcgta tgcatgcttt gcatacttct gcctgctggg
7381 gagcctgggg actttccaca ccctaactga cacacattcc acagaattcc ttgaaataca
7441 tccacaggta cacctccaat tgactcaaat gatgtcaatt agtctatcag aagcttctaa
7501 agccatgaca tcattttctg gaattttcca agctgtttaa aggcacagtc aacttagtgt
7561 atgtaaactt ctgacccact ggaattgtga tacagtgaat tataagtgaa ataatctgtc
7621 tgtaaacaat tgttggaaaa atgacttgtg tcatgcacaa agtagatgtc ctaactgact
7681 tgccaaaact attgtttgtt aacaagaaat ttgtggagta gttgaaaaac gagttttaat
7741 gactccaact taagtgtatg taaacttccg acttcaactg tagggtaccg agctcgaatt
7801 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
7861 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
7921 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
7981 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
8041 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
8101 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
8161 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
8221 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
8281 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
8341 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
8401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
8461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
8521 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
8581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
8641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
8701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
8761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
8821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
8881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
8941 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
9001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
9061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
9121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
9181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
9241 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
9301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
9361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
9421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
9481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
9541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
9601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
9661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
9721 cggataaggc gcagcggtcg ggc
//
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