| 出品公司: | Addgene |
|---|---|
| 载体名称: | PX389 或 PX389 Fluviicola taffensis Cas9 |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 11570 bp |
| 5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
| 载体标签: | NLS (C ter)；HA、NLS (N ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 昆虫细胞 |
| 备注: | -- |
| 产品目录号: | #68326 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
PX389载体质粒基本信息
PX389质粒图谱载体图谱和PX389载体序列质粒序列多克隆位点信息
PX389质粒载体简介
PX389质粒序列载体序列
LOCUS PX389 Fluviicola taffensis Cas9 11570 bp DNA SYN
DEFINITION PX389 Fluviicola taffensis Cas9
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11570
/organism="PX389 Fluviicola taffensis Cas9"
/mol_type="other DNA"
misc_feature 47..1001
/label="Ac_5_flank"
CDS complement(343..948)
/label="ORF frame 3"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1023..1587
/label="CMV_immearly_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1090..1377
/label="CAG_enhancer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1544..1564
/label="CMV_fwd_primer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1615..1633
/label="T7_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1633..1656
/label="lacO"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1772..1796
/label="attB1"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
CDS complement(1795..2502)
/label="ORF frame 3"
/translation="MKLHQLIWCLFLFLASQLIVSPFLIEVQQDPCQFLLLNFLRQSF
LSQIVDCPVMGNFLAVAHQLWLVDFKVDQHFLVALLKQEHVISIETDFWLRFRLELAE
PLLKVNLVGIVFWQEANDIQHSMQPLAPQQSSVDQSPDSPQSSSLSCLTDRVAFSELV
LNILCHRNDPAAHAQDLPLFVIEVLVNQCPSNAVSPQIQSQNSLCHGRFDLALFLRRS
VIWNIVWVHGGTGGISP*"
CDS 1822..6261
/label="ORF frame 1"
/translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
misc_feature 1825..1851
/label="HA"
/translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
misc_feature 1855..1875
/label="SV40 NLS"
/translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
misc_feature 1861..1875
/label="NLS"
/translation="MYPYDVPDYASPKKKRKVEASMTKTILGLDLGTNSIGWALINQD
FDNKKGEILGMGSRIIPMTQDIKDEFGKGNSISQTAERTRLRGVRRLIHRTLLRRERL
HRVLNIIGFLPEHYANQIDFEKRFGKFKPESEPKISFDGNDVFLFEKSYQEMLVDFKI
HQPQLVSNGKKIPHDWTIYYLRKKALSKKIEKEELAWILLNFNQKRGYYQLRGEEEEE
TPNKLVEFHSLKIVEVNADEPQKGKPEIWYSLVLENGWVYRRASKTPLFDWKDKIREF
IVTTEINNDGTVKTDKEGTEKRSFRAPKPEDWTLQKKKTEFELSKSGNEVGAFIYESI
LQKPNQKIRGKLISTIERKFYKEELKTILKTQLFFHKELKDEKLYNACIEELYKNNEA
HRSLLSNKGFEHLFINDILFYQRPLRSKKSQISNCPLEKRTYKKEGIEITEGIKVISK
SNPIYQEFRLWQWISNLSLYCIEPTETNVTSTFLNSIEDYENLFEFLNNRKEIEQKHL
LKYLLENQGFKGKLLTNELEKFRWNFVADKKYPCNETGSLLHTRLSKVKNISPDFLTK
EIEHQLWHIIYSVTDKIEYEQALKTFARKNNLDVDSFFEHFKKIPPFESTYGAYSEKA
IKKLLPLIRLGKYWNWEAIDSISKDRISKILSGEYDENIKNRVREKAVHLTSENNFQG
LQEWLAKYIVYDRHSEGNDLGKWTSVSDLETYLKEFKQHSLRNPIVEQVITETLRVVK
DIWIKHGKGTENFFDEIHVELGREMKNNSEDRKRLTNTITENENTNLRIKALLMEMMN
DNDVENVRPYSPSQQEILKIYEDGALNSNIELDDEIVKISKKAEPTKSELQRYKLWLE
QKYRSPYTGQVIPLNKLFTSEYEIEHVVPQSRFFDDSFSNKVICESAVNKRKDNQLGL
QFIKNHSGEKVELGFGKVVQVFTEEQYLDFVKEHYSKNRSKHNKLLLEEIPEKMIERQ
LNDTRYISKFVSSILSNIVRSEKDDDGLNSKNILPGNGKITTELKRDWGLNDVWNDLI
LPRFERMNTITNSDLFTTYNDKYQKHLPTVPFEYSKGFQKKRIDHRHHAMDALVIACA
TRDHLNLMNNQSAKSELKRYDLQNKLRKKEPYFNQKENKQKEAFKDFIKPWGTFTEDS
KNALEKIIISFKQNLRVINKATNSYESYKDEDGNLRIGKDGKPEKGLIKQKGLNWAIR
KPLHKDTVSGQINLSRIKLPNGKILTATRKNLDTSFDLKTIENSITDTGIQKILKNYL
LSKESPELAFSPEGLEEMNNEIEKYNNGKFHHPINKARIYELGSKFNVGQTGNRRDKF
VEAAKGTNLFFAIYQDENKNRSYETIPLNEVIEHQKWRASLPKEEQEKIPLVPVNKLK
GTFIFSLSPNDLVYVPSNDELERSASIDFSKLKKEQINRLYKMVSSSGSQCFFVKSEV
ATSVVNKMEYSSLNKMEKSIDNLMVKEICIKLKIDRLGNISKA*"
CDS complement(4525..5097)
/label="ORF frame 3"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
misc_feature complement(6294..6311)
/label="Sp6_primer"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
misc_feature 6316..6340
/label="attB2"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
terminator 6352..6818
/label="hGH_PA_terminator"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
terminator 6824..6859
/label="T7_terminator"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
rep_origin 6976..7282
/label="f1_origin"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
misc_feature complement(7396..7416)
/label="pBABE_3_primer"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
misc_feature complement(7402..7617)
/label="SV40_enhancer"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
promoter 7414..7682
/label="SV40_promoter"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
rep_origin 7581..7658
/label="SV40_origin"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
misc_feature 7643..7662
/label="SV40pro_F_primer"
/translation="MIPCGTCDDQCVHSMVSVINALFLKALGVLERHGGQVFLILVVV
SCKQIAVCDGIHPLESWQNQVVPHIIQPPVPFQFGSDLAISRQNVFAIQAVIVLLRSD
DVGQNGAHELTDVASVIQLPFDHLLGNLFQQQFVMLGAIFAIVLLHKIQVLLFCKDLD
HLPEAQLHFFSAMVLDELQPQLVILPLVDG*"
CDS 7797..8591
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 7800..8588
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(8106..8642)
/label="ORF frame 1"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 8768..8887
/label="SV40_PA_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 8856..8875
/label="EBV_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 9078..9113
/label="T7_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature complement(9136..9153)
/label="Baculovirs_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 9143..9220
/label="3_AcPH"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS complement(9250..9867)
/label="ORF frame 3"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
misc_feature 9847..9880
/label="loxP"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
gene complement(10648..11508)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
CDS complement(10648..11508)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatataccA CCGGTGCCAC CATGTACCCA TACGATGTTC CAGATTACGC TTCGCCGAAG
1861 AAAAAGCGCA AGGTCGAAGC GTCCATGACA AAGACTATTC TGGGACTGGA TCTGGGGACT
1921 AACAGCATTG GATGGGCACT GATTAACCAG GACTTCGATA ACAAAAAGGG GGAGATCCTG
1981 GGCATGGGCA GCCGGATCAT TCCTATGACA CAGGATATTA AGGACGAGTT CGGAAAAGGC
2041 AACTCTATCA GTCAGACAGC TGAGCGAACT CGACTGCGGG GAGTCCGGAG ACTGATCCAC
2101 AGAACTCTGC TGAGGCGCGA GCGGCTGCAT AGAGTGCTGA ATATCATTGG CTTCCTGCCA
2161 GAACACTATG CCAACCAGAT TGACTTTGAG AAGAGGTTCG GCAAGTTCAA GCCTGAATCT
2221 GAGCCAAAAA TCAGTTTCGA TGGAAATGAC GTGTTCCTGT TTGAGAAGAG CTACCAGGAA
2281 ATGCTGGTCG ACTTTAAAAT CCACCAGCCA CAGCTGGTGA GCAACGGCAA GAAAATTCCC
2341 CATGACTGGA CAATCTACTA TCTGAGAAAG AAAGCTCTGT CTAAGAAAAT TGAGAAGGAG
2401 GAACTGGCAT GGATCCTGCT GAACTTCAAT CAGAAACGGG GATACTATCA GCTGAGAGGC
2461 GAGGAAGAGG AAGAGACACC AAATAAGCTG GTGGAGTTTC ATAGCCTGAA AATTGTGGAG
2521 GTCAACGCCG ATGAACCCCA GAAAGGAAAG CCTGAGATCT GGTACTCACT GGTGCTGGAA
2581 AACGGCTGGG TCTATCGACG GGCTAGCAAG ACTCCCCTGT TCGATTGGAA AGACAAGATT
2641 AGGGAGTTTA TCGTGACCAC AGAAATCAAC AATGATGGAA CTGTCAAAAC CGACAAGGAG
2701 GGCACCGAAA AGAGGAGCTT CCGCGCACCA AAACCCGAGG ACTGGACTCT GCAGAAGAAA
2761 AAGACCGAGT TCGAGCTGTC AAAGAGCGGG AATGAAGTGG GAGCCTTCAT CTACGAAAGT
2821 ATCCTGCAGA AACCCAACCA GAAGATTAGA GGCAAACTGA TTTCAACCAT CGAGAGGAAA
2881 TTTTATAAGG AAGAGCTGAA AACCATCCTG AAAACACAGC TGTTCTTTCA CAAAGAACTG
2941 AAGGATGAGA AACTGTACAA TGCCTGCATC GAAGAGCTGT ATAAGAACAA TGAAGCTCAC
3001 CGGAGCCTGC TGTCCAACAA GGGGTTCGAG CATCTGTTTA TTAACGACAT CCTGTTCTAC
3061 CAGCGACCTC TGCGGTCTAA AAAGAGTCAG ATCTCAAACT GCCCACTGGA GAAGCGCACA
3121 TATAAAAAGG AGGGGATTGA AATCACTGAG GGCATCAAAG TGATCTCCAA ATCTAATCCA
3181 ATCTACCAGG AGTTCCGGCT GTGGCAGTGG ATTAGCAACC TGTCCCTGTA TTGTATCGAA
3241 CCCACCGAGA CAAATGTGAC TTCAACCTTT CTGAACAGCA TTGAAGATTA CGAGAATCTG
3301 TTCGAATTTC TGAACAATCG CAAGGAAATC GAGCAGAAGC ACCTGCTGAA ATATCTGCTG
3361 GAGAACCAGG GGTTTAAAGG AAAGCTGCTG ACAAACGAAC TGGAGAAGTT CCGCTGGAAT
3421 TTTGTCGCTG ACAAAAAGTA CCCCTGTAAT GAGACAGGCA GCCTGCTGCA TACTCGGCTG
3481 AGCAAAGTGA AGAACATTTC CCCTGATTTC CTGACCAAGG AAATCGAGCA CCAGCTGTGG
3541 CATATCATCT ACAGCGTGAC CGACAAGATT GAATATGAGC AGGCCCTGAA AACCTTTGCT
3601 CGGAAAAACA ATCTGGATGT GGACTCCTTC TTTGAGCACT TCAAAAAGAT CCCCCCTTTT
3661 GAGTCTACCT ACGGAGCATA TAGTGAAAAG GCCATCAAGA AGCTGCTGCC ACTGATCAGA
3721 CTGGGCAAAT ACTGGAACTG GGAGGCCATT GATAGTATCT CAAAGGACAG GATTAGTAAA
3781 ATCCTGTCAG GGGAATACGA TGAGAACATT AAGAACAGAG TGAGGGAGAA AGCAGTCCAC
3841 CTGACCTCCG AAAACAATTT CCAGGGACTG CAGGAGTGGC TGGCCAAGTA CATCGTCTAT
3901 GATCGCCATT CTGAGGGCAA TGACCTGGGG AAGTGGACTA GCGTGTCCGA CCTGGAGACA
3961 TACCTGAAGG AGTTCAAGCA GCATAGCCTG CGGAACCCTA TTGTGGAGCA GGTCATCACA
4021 GAAACTCTGA GAGTGGTCAA GGATATTTGG ATCAAGCACG GGAAAGGAAC CGAAAATTTC
4081 TTTGACGAAA TCCATGTGGA GCTGGGCCGG GAAATGAAGA ACAATTCCGA GGATCGCAAA
4141 CGACTGACCA ACACAATTAC TGAAAACGAG AATACAAACC TGAGAATCAA GGCCCTGCTG
4201 ATGGAAATGA TGAATGATAA CGACGTGGAG AACGTCAGGC CTTACTCTCC AAGTCAGCAG
4261 GAGATTCTGA AGATCTATGA GGACGGAGCT CTGAATAGCA ACATCGAGCT GGACGATGAA
4321 ATTGTGAAGA TCTCCAAAAA GGCAGAGCCC ACCAAATCTG AACTGCAGCG CTACAAGCTG
4381 TGGCTGGAGC AGAAATACCG ATCCCCTTAT ACTGGCCAGG TCATCCCACT GAACAAGCTG
4441 TTCACCTCTG AATATGAGAT CGAACACGTG GTCCCTCAGA GTCGCTTCTT TGACGATAGC
4501 TTCAGCAACA AAGTGATCTG CGAGTCAGCC GTCAACAAGC GGAAGGATAA CCAGCTGGGG
4561 CTGCAGTTCA TCAAGAACCA TAGCGGAGAA AAAGTGGAGC TGGGCTTCGG GAAGGTGGTC
4621 CAGGTCTTTA CAGAAGAGCA GTACCTGGAT TTTGTGAAGG AGCACTATAG CAAAAATCGC
4681 TCCAAGCATA ACAAACTGCT GCTGGAAGAG ATTCCCGAGA AGATGATCGA AAGGCAGCTG
4741 AATGACACTC GCTACATCAG TAAGTTCGTG AGCTCCATTC TGTCCAACAT CGTCAGATCT
4801 GAGAAGGACG ATGACGGCCT GAATAGCAAA AACATTCTGC CTGGAAATGG CAAGATCACT
4861 ACCGAACTGA AAAGGGACTG GGGGCTGAAT GATGTGTGGA ACGACCTGAT TCTGCCAAGA
4921 TTCGAGAGGA TGAATACCAT CACAAACAGC GATCTGTTTA CAACTTACAA CGACAAGTAT
4981 CAGAAACACC TGCCCACCGT GCCTTTCGAG TACTCCAAGG GCTTTCAGAA AAAGCGCATT
5041 GATCACCGAC ACCATGCTAT GGACGCACTG GTCATCGCAT GTGCCACACG GGATCATCTG
5101 AATCTGATGA ACAATCAGTC TGCCAAGAGT GAACTGAAAC GATACGACCT GCAGAACAAG
5161 CTGCGGAAAA AGGAGCCTTA CTTCAACCAG AAGGAGAACA AACAGAAGGA AGCCTTCAAG
5221 GATTTTATCA AACCATGGGG CACTTTCACC GAGGACAGCA AGAATGCTCT GGAAAAAATC
5281 ATTATCTCCT TTAAGCAGAA CCTGAGAGTG ATCAACAAGG CAACAAACTC ATACGAGAGC
5341 TATAAGGATG AGGACGGGAA TCTGAGGATC GGCAAGGATG GGAAACCAGA GAAGGGCCTG
5401 ATCAAGCAGA AGGGGCTGAA CTGGGCAATC AGAAAGCCCC TGCACAAAGA CACCGTGTCA
5461 GGCCAGATTA ACCTGAGCAG GATCAAGCTG CCCAATGGGA AAATCCTGAC AGCCACTCGC
5521 AAGAATCTGG ATACCAGTTT CGACCTGAAA ACAATTGAGA ACTCAATCAC CGACACAGGC
5581 ATTCAGAAAA TCCTGAAGAA TTACCTGCTG TCCAAGGAAT CTCCAGAGCT GGCCTTCTCT
5641 CCCGAGGGCC TGGAAGAGAT GAACAATGAG ATCGAAAAGT ACAACAATGG GAAATTTCAC
5701 CATCCTATTA ACAAGGCTAG GATCTATGAA CTGGGGAGCA AATTCAATGT CGGACAGACA
5761 GGCAACAGAA GGGATAAGTT TGTGGAGGCC GCTAAAGGAA CTAATCTGTT CTTTGCTATC
5821 TACCAGGACG AGAATAAGAA CCGCTCTTAT GAAACTATTC CCCTGAACGA AGTGATCGAG
5881 CACCAGAAGT GGCGAGCAAG TCTGCCTAAG GAAGAGCAGG AGAAAATTCC ACTGGTGCCC
5941 GTCAACAAGC TGAAGGGGAC CTTCATCTTT TCCCTGTCTC CCAACGATCT GGTGTACGTC
6001 CCTTCCAATG ACGAGCTGGA ACGAAGTGCT TCAATTGACT TCTCTAAGCT GAAAAAGGAA
6061 CAGATCAACC GGCTGTATAA AATGGTGTCT AGTTCAGGAT CCCAGTGCTT CTTTGTGAAG
6121 AGTGAGGTCG CAACCTCAGT GGTCAACAAA ATGGAATACA GCAGCCTGAA CAAGATGGAG
6181 AAGTCTATCG ATAACCTGAT GGTGAAGGAG ATTTGTATCA AACTGAAGAT TGACAGGCTG
6241 GGCAACATCA GCAAGGCCTA AGAATTCggt accgagctca agcttgcggc cgcctatagt
6301 gtcacctaaa tcgtaaccca gctttcttgt acaaagtggt cgggtggcat ccctgtgacc
6361 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
6421 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
6481 ggaggggggt ggtatggagc aaggggcaag ttgggatgac aacctgtagg gcctgcgggg
6541 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
6601 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
6661 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
6721 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
6781 gattacaggc gtgaaccact gctcccttcc ctgtccttct agcataaccc cttggggcct
6841 ctaaacgggt cttgaggggt tttttgtcta gaagacaata gcaggcatgc tggggatgcg
6901 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
6961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
7021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
7081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
7141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
7201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
7261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
7321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
7381 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
7441 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
7501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
7561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
7621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
7681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
7741 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
7801 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
7861 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
7921 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
7981 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
8041 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
8101 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
8161 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
8221 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
8281 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
8341 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
8401 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
8461 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
8521 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
8581 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
8641 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
8701 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
8761 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
8821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
8881 atcttatcat gtctgttcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
8941 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
9001 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
9061 gcccatatgt aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
9121 ctgaaagcat gcggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt
9181 tgcaccagac gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta
9241 gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta
9301 ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg
9361 attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag
9421 gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg
9481 attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat
9541 attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt
9601 tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag
9661 agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc
9721 ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct
9781 attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattgc
9841 gatcgcataa cttcgtatag catacattat acgaagttat aagctcggaa cgctgcgctc
9901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
9961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
10021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac
10081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
10141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
10201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
10261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
10321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
10381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
10441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
10501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
10561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
10621 aaaaaaggat ctcaagaaga tcctttgtta ccaatgctta atcagtgagg cacctatctc
10681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
10741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
10801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
10861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
10921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
10981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
11041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
11101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
11161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
11221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
11281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
11341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
11401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
11461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
11521 tcaatattat tgaagcattt atcagggtta ttgtctcatg tccgcgcgtt
//
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PX389质粒载体应用举例
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