pBBR1MCS-6

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品人: Meng,J., Wang,H.
载体名称: pBBR1MCS-6
质粒类型: 广宿主载体;克隆载体;原核表达载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: lac
载体大小: 5859 bp
5' 测序引物及序列: M13 Forward 5’TGTAAAACGACGGCCAGT 3’
3' 测序引物及序列: M13 Reverse 5’CAGGAAACAGCTATGAC 3’
载体标签: --
载体抗性: 链霉素
克隆菌株: DH5α 等
宿主细胞(系): 多个菌属
备注: 可用蓝白斑筛选法
产品目录号: --
组成型/诱导型: 诱导型

载体质粒图谱和多克隆位点信息

pBBR1MCS-6载体图谱



pBBR1MCS-6 多克隆位点

载体简介

载体序列

LOCUS       KC698875                5859 bp    DNA     circular SYN 26-AUG-2013
DEFINITION  Cloning vector pBBR1MCS-6, complete sequence.
ACCESSION   KC698875
VERSION     KC698875.1  GI:531997060
  AUTHORS   Meng,J., Wang,H., Liu,X., Lin,J., Pang,X. and Lin,J.
  TITLE     Construction of small plasmid vectors for use in genetic
            improvement of the extremely acidophilic Acidithiobacillus caldus
  JOURNAL   Submitted (27-FEB-2013) State Key Laboratory of Microbial
            Technology, Shandong University, Shanda Nanlu 27#, Jinan, Shandong
            250100, China
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Cloning vector pBBR1MCS-6"
                     /mol_type="other DNA"
                     /db_xref="taxon:1387373"
     gene            complement(join(5660..5859,1..799))
                     /gene="mob"
     CDS             complement(join(5660..5859,1..799))
                     /gene="mob"
                     /note="required for plasmid mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="Mob"
                     /protein_id="AGT62526.1"
                     /db_xref="GI:531997062"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLG"
     gene            1793..2455
                     /gene="rep"
     CDS             1793..2455
                     /gene="rep"
                     /note="required for pBBR1 derivative plasmid replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AGT62525.1"
                     /db_xref="GI:531997061"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            complement(3013..3378)
                     /gene="lacZ"
     CDS             complement(3013..3378)
                     /gene="lacZ"
                     /note="beta-galactosidase; alpha peptide of LacZ"
                     /codon_start=1
                     /transl_table=11
                     /product="LacZ"
                     /protein_id="AGT62527.1"
                     /db_xref="GI:531997063"
                     /translation="MTMITPSAQLTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPL
                     VLERPPPRWSSNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWKL"
     gene            3682..4485
                     /gene="strA"
     CDS             3682..4485
                     /gene="strA"
                     /codon_start=1
                     /transl_table=11
                     /product="Sm resistance protein A"
                     /protein_id="AGT62528.1"
                     /db_xref="GI:531997064"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4485..5321
                     /gene="strB"
     CDS             4485..5321
                     /gene="strB"
                     /codon_start=1
                     /transl_table=11
                     /product="Sm resistance protein B"
                     /protein_id="AGT62529.1"
                     /db_xref="GI:531997065"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
ORIGIN      
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
     3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcatt
     3601 catgtttgac agcttatcat ccataaggtt taatgcggta gtttatcaca gttaaattgc
     3661 taacgcagtc aggcaccgtg tatgaatcga actaatattt tttttggtga atcgcattct
     3721 gactggttgc ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg
     3781 catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac
     3841 cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag
     3901 gaacaggagg gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg
     3961 tctggagcgg atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac
     4021 agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc
     4081 gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt
     4141 acgccgctgc acgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa
     4201 gagcgcaccg atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac
     4261 cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc
     4321 tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa
     4381 gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa
     4441 cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg
     4501 tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc
     4561 tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta
     4621 tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg
     4681 gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc
     4741 gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg
     4801 aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga
     4861 tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc
     4921 aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac
     4981 tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc
     5041 tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct
     5101 acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg
     5161 acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt
     5221 atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta
     5281 tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct
     5341 atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg
     5401 attccgcccc ttcccgcaaa cgaccccaaa caagatcttg atcccctgcg ccatcagatc
     5461 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
     5521 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
     5581 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
     5641 atagcccagt agctgacatt catcccaggt ggcacttttc ggggaaatgt gcgcgcccgc
     5701 gttcctgctg gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct
     5761 tttcgcccac ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc
     5821 ccagggcgtc cagaacgggc ttcaggcgct cccgaaggt
//

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