LOCUS pSG5-HA-p300 11977 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mammalian expression of full-length human p300 in pSG5.
ACCESSION .
VERSION .
KEYWORDS pSG5-HA-p300
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11977)
AUTHORS Askew EB, Bai S, Blackwelder AJ, Wilson EM
TITLE Transcriptional synergy between melanoma antigen gene protein-A11
(MAGE-11) and p300 in androgen receptor signaling.
JOURNAL J Biol Chem. 2010 Jul 9;285(28):21824-36. doi:
10.1074/jbc.M110.120600. Epub 2010 May 6.
PUBMED 20448036
REFERENCE 2 (bases 1 to 11977)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11977
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..211
/label=SV40 promoter
/note="SV40 early promoter"
promoter 23..219
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 70..205
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 132..151
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 250..822
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 830..849
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind 877..896
/label=T7
/note="T7 promoter, forward primer"
promoter 877..895
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 915..931
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 915..931
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 942..968
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 4110..5960
/codon_start=1
/gene="EP300"
/product="catalytic histone acetyltransferase core of the
human E1A-associated protein p300 (Delvecchio et al.,
2013)"
/label=p300 core
/translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
GNFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
regulatory 5724..5733
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 8839..8960
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8876..8895)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8930..8949
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8994..9011)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9165..9753)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9245..9264)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9924..10784)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10547..10566
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10785..10889)
/gene="bla"
/label=AmpR promoter
primer_bind 10957..10975
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(11013..11035)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 11135..11154
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(11221..11676)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(11358..11379)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 11570..11589
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 11802..11824
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 11816..11833
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 11817..11833
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11870..11890)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
901 ttcggatcgg ccgctctaga actagtggat ccaccatggc atacccatac gacgtgcctg
961 actacgcctc cgccgagaat gtggtggaac cggggccgcc ttcagccaag cggcctaaac
1021 tctcatctcc ggccctctcg gcgtccgcca gcgatggcac agattttggc tctctatttg
1081 acttggagca cgacttacca gatgaattaa tcaactctac agaattggga ctaaccaatg
1141 gtggtgatat taatcagctt cagacaagtc ttggcatggt acaagatgca gcttctaaac
1201 ataaacagct gtcagaattg ctgcgatctg gtagttcccc taacctcaat atgggagttg
1261 gtggcccagg tcaagtcatg gccagccagg cccaacagag cagtcctgga ttaggtttga
1321 taaatagcat ggtcaaaagc ccaatgacac aggcaggctt gacttctccc aacatgggga
1381 tgggcactag tggaccaaat cagggtccta cgcagtcaac aggtatgatg aacagtccag
1441 taaatcagcc tgccatggga atgaacacag ggatgaatgc gggcatgaat cctggaatgt
1501 tggctgcagg caatggacaa gggataatgc ctaatcaagt catgaacggt tcaattggag
1561 caggccgagg gcgacagaat atgcagtacc caaacccagg catgggaagt gctggcaact
1621 tactgactga gcctcttcag cagggctctc cccagatggg aggacaaaca ggattgagag
1681 gcccccagcc tcttaagatg ggaatgatga acaaccccaa tccttatggt tcaccatata
1741 ctcagaatcc tggacagcag attggagcca gtggccttgg tctccagatt cagacaaaaa
1801 ctgtactatc aaataactta tctccatttg ctatggacaa aaaggcagtt cctggtggag
1861 gaatgcccaa catgggtcaa cagccagccc cgcaggtcca gcagccaggt ctggtgactc
1921 cagttgccca agggatgggt tctggagcac atacagctga tccagagaag cgcaagctca
1981 tccagcagca gcttgttctc cttttgcatg ctcacaagtg ccagcgccgg gaacaggcca
2041 atggggaagt gaggcagtgc aaccttcccc actgtcgcac aatgaagaat gtcctaaacc
2101 acatgacaca ctgccagtca ggcaagtctt gccaagtggc acactgtgca tcttctcgac
2161 aaatcatttc acactggaag aattgtacaa gacatgattg tcctgtgtgt ctccccctca
2221 aaaatgctgg tgataagaga aatcaacagc caattttgac tggagcaccc gttggacttg
2281 gaaatcctag ctctctaggg gtgggtcaac agtctgcccc caacctaagc actgttagtc
2341 agattgatcc cagctccata gaaagagcct atgcagctct tggactaccc tatcaagtaa
2401 atcagatgcc gacacaaccc caggtgcaag caaagaacca gcagaatcag cagcctgggc
2461 agtctcccca aggcatgcgg cccatgagca acatgagtgc tagtcctatg ggagtaaatg
2521 gaggtgtagg agttcaaacg ccgagtcttc tttctgactc aatgttgcat tcagccataa
2581 attctcaaaa cccaatgatg agtgaaaatg ccagtgtgcc ctccctgggt cctatgccaa
2641 cagcagctca accatccact actggaattc ggaaacagtg gcacgaagat attactcagg
2701 atcttcgaaa tcatcttgtt cacaaactcg tccaagccat atttcctacg ccggatcctg
2761 ctgctttaaa agacagacgg atggaaaacc tagttgcata tgctcggaaa gttgaagggg
2821 acatgtatga atctgcaaac aatcgagcgg aatactacca ccttctagct gagaaaatct
2881 ataagatcca gaaagaacta gaagaaaaac gaaggaccag actacagaag cagaacatgc
2941 taccaaatgc tgcaggcatg gttccagttt ccatgaatcc agggcctaac atgggacagc
3001 cgcaaccagg aatgacttct aatggccctc tacctgaccc aagtatgatc cgtggcagtg
3061 tgccaaacca gatgatgcct cgaataactc cacaatctgg tttgaatcaa tttggccaga
3121 tgagcatggc ccagccccct attgtacccc ggcaaacccc tcctcttcag caccatggac
3181 agttggctca acctggagct ctcaacccgc ctatgggcta tgggcctcgt atgcaacagc
3241 cttccaacca gggccagttc cttcctcaga ctcagttccc atcacaggga atgaatgtaa
3301 caaatatccc tttggctccg tccagcggtc aagctccagt gtctcaagca caaatgtcta
3361 gttcttcctg cccggtgaac tctcctataa tgcctccagg gtctcagggg agccacattc
3421 actgtcccca gcttcctcaa ccagctcttc atcagaattc accctcgcct gtacctagtc
3481 gtacccccac ccctcaccat actcccccaa gcataggggc tcagcagcca ccagcaacaa
3541 caattccagc ccctgttcct acacctcctg ccatgccacc tgggccacag tcccaggctc
3601 tacatccccc tccaaggcag acacctacac caccaacaac acaacttccc caacaagtgc
3661 agccttcact tcctgctgca ccttctgctg accagcccca gcagcagcct cgctcacagc
3721 agagcacagc agcgtctgtt cctaccccaa cagcaccgct gcttcctccg cagcctgcaa
3781 ctccactttc ccagccagct gtaagcattg aaggacaggt atcaaatcct ccatctacta
3841 gtagcacaga agtgaattct caggccattg ctgagaagca gccttcccag gaagtgaaga
3901 tggaggccaa aatggaagtg gatcaaccag aaccagcaga tactcagccg gaggatattt
3961 cagagtctaa agtggaagac tgtaaaatgg aatctaccga aacagaagag agaagcactg
4021 agttaaaaac tgaaataaaa gaggaggaag accagccaag tacttcagct acccagtcat
4081 ctccggctcc aggacagtca aagaaaaaga ttttcaaacc agaagaacta cgacaggcac
4141 tgatgccaac attggaggca ctttaccgtc aggatccaga atcccttccc tttcgtcaac
4201 ctgtggaccc tcagctttta ggaatccctg attactttga tattgtgaag agccccatgg
4261 atctttctac cattaagagg aagttagaca ctggacagta tcaggagccc tggcagtatg
4321 tcgatgatat ttggcttatg ttcaataatg cctggttata taaccggaaa acatcacggg
4381 tatacaaata ctgctccaag ctctctgagg tctttgaaca agaaattgac ccagtgatgc
4441 aaagccttgg atactgttgt ggcagaaagt tggagttctc tccacagaca ctgtgttgct
4501 acggcaaaca gttgtgcaca atacctcgtg atgccactta ttacagttac cagaacaggt
4561 atcatttctg tgagaagtgt ttcaatgaga tccaagggga gagcgtttct ttgggggatg
4621 acccttccca gcctcaaact acaataaata aagaacaatt ttccaagaga aaaaatgaca
4681 cactggatcc tgaactgttt gttgaatgta cagagtgcgg aagaaagatg catcagatct
4741 gtgtccttca ccatgagatc atctggcctg ctggattcgt ctgtgatggc tgtttaaaga
4801 aaagtgcacg aactaggaaa gaaaataagt tttctgctaa aaggttgcca tctaccagac
4861 ttggcacctt tctagagaat cgtgtgaatg actttctgag gcgacagaat caccctgagt
4921 caggagaggt cactgttaga gtagttcatg cttctgacaa aaccgtggaa gtaaaaccag
4981 gcatgaaagc aaggtttgtg gacagtggag agatggcaga atcctttcca taccgaacca
5041 aagccctctt tgcctttgaa gaaattgatg gtgttgacct gtgcttcttt ggcatgcatg
5101 ttcaagagta tggctctgac tgccctccac ccaaccagag gagagtatac atatcttacc
5161 tcgatagtgt tcatttcttc cgtcctaaat gcttgaggac tgcagtctat catgaaatcc
5221 taattggata tttagaatat gtcaagaaat taggttacac aacagggcat atttgggcat
5281 gtccaccaag tgagggagat gattatatct tccattgcca tcctcctgac cagaagatac
5341 ccaagcccaa gcgactgcag gaatggtaca aaaaaatgct tgacaaggct gtatcagagc
5401 gtattgtcca tgactacaag gatattttta aacaagctac tgaagataga ttaacaagtg
5461 caaaggaatt gccttatttc gagggtaatt tctggcccaa tgttctggaa gaaagcatta
5521 aggaactgga acaggaggaa gaagagagaa aacgagagga aaacaccagc aatgaaagca
5581 cagatgtgac caagggagac agcaaaaatg ctaaaaagaa gaataataag aaaaccagca
5641 aaaataagag cagcctgagt aggggcaaca agaagaaacc cgggatgccc aatgtatcta
5701 acgacctctc acagaaacta tatgccacca tggagaagca taaagaggtc ttctttgtga
5761 tccgcctcat tgctggccct gctgccaact ccctgcctcc cattgttgat cctgatcctc
5821 tcatcccctg cgatctgatg gatggtcggg atgcgtttct cacgctggca agggacaagc
5881 acctggagtt ctcttcactc cgaagagccc agtggtccac catgtgcatg ctggtggagc
5941 tgcacacgca gagccaggac cgctttgtct acacctgcaa tgaatgcaag caccatgtgg
6001 agacacgctg gcactgtact gtctgtgagg attatgactt gtgtatcacc tgctataaca
6061 ctaaaaacca tgaccacaaa atggagaaac taggccttgg cttagatgat gagagcaaca
6121 accagcaggc tgcagccacc cagagcccag gcgattctcg ccgcctgagt atccagcgct
6181 gcatccagtc tctggtccat gcttgccagt gtcggaatgc caattgctca ctgccatcct
6241 gccagaagat gaagcgggtt gtgcagcata ccaagggttg caaacggaaa accaatggcg
6301 ggtgccccat ctgcaagcag ctcattgccc tctgctgcta ccatgccaag cactgccagg
6361 agaacaaatg cccggtgccg ttctgcctaa acatcaagca gaagctccgg cagcaacagc
6421 tgcagcaccg actacagcag gcccaaatgc ttcgcaggag gatggccagc atgcagcgga
6481 ctggtgtggt tgggcagcaa cagggcctcc cttcccccac tcctgccact ccaacgacac
6541 caactggcca acagccaacc accccgcaga cgccccagcc cacttctcag cctcagccta
6601 cccctcccaa tagcatgcca ccctacttgc ccaggactca agctgctggc cctgtgtccc
6661 agggtaaggc agcaggccag gtgacccctc caacccctcc tcagactgct cagccacccc
6721 ttccagggcc cccacctgca gcagtggaaa tggcaatgca gattcagaga gcagcggaga
6781 cgcagcgcca gatggcccac gtgcaaattt ttcaaaggcc aatccaacac cagatgcccc
6841 cgatgactcc catggccccc atgggtatga acccacctcc catgaccaga ggtcccagtg
6901 ggcatttgga gccagggatg ggaccgacag ggatgcagca acagccaccc tggagccaag
6961 gaggattgcc tcagccccag caactacagt ctgggatgcc aaggccagcc atgatgtcag
7021 tggcccagca tggtcaacct ttgaacatgg ctccacaacc aggattgggc caggtaggta
7081 tcagcccact caaaccaggc actgtgtctc aacaagcctt acaaaacctt ttgcggactc
7141 tcaggtctcc cagctctccc ctgcagcagc aacaggtgct tagtatcctt cacgccaacc
7201 cccagctgtt ggctgcattc atcaagcagc gggctgccaa gtatgccaac tctaatccac
7261 aacccatccc tgggcagcct ggcatgcccc aggggcagcc agggctacag ccacctacca
7321 tgccaggtca gcagggggtc cactccaatc cagccatgca gaacatgaat ccaatgcagg
7381 cgggcgttca gagggctggc ctgccccagc agcaaccaca gcagcaactc cagccaccca
7441 tgggagggat gagcccccag gctcagcaga tgaacatgaa ccacaacacc atgccttcac
7501 aattccgaga catcttgaga cgacagcaaa tgatgcaaca gcagcagcaa cagggagcag
7561 ggccaggaat aggccctgga atggccaacc ataaccagtt ccagcaaccc caaggagttg
7621 gctacccacc acagcagcag cagcggatgc agcatcacat gcaacagatg caacaaggaa
7681 atatgggaca gataggccag cttccccagg ccttgggagc agaggcaggt gccagtctac
7741 aggcctatca gcagcgactc cttcagcaac agatggggtc ccctgttcag cccaacccca
7801 tgagccccca gcagcatatg ctcccaaatc aggcccagtc cccacaccta caaggccagc
7861 agatccctaa ttctctctcc aatcaagtgc gctctcccca gcctgtccct tctccacggc
7921 cacagtccca gcccccccac tccagtcctt ccccaaggat gcagcctcag ccttctccac
7981 accacgtttc cccacagaca agttccccac atcctggact ggtagctgcc caggccaacc
8041 ccatggaaca agggcatttt gccagcccgg accagaattc aatgctttct cagcttgcta
8101 gcaatccagg catggcaaac ctccatggtg caagcgccac ggacctggga ctcagcaccg
8161 ataactcaga cttgaattca aacctctcac agagtacact agacatacac tagagacacc
8221 ttgtagtatt ttgggagcaa aaaaattatt ttctcttaac aagacttttt gtactgaaaa
8281 caattttttt gaatctttcg tagcctaaaa gacaattttc cttggaacac ataagaactg
8341 tgcagtagcc gtttgtggtt taaagcaaac atgcaagatg aacctgaggg atgatagaat
8401 acaaagaata tatttttgtt atggctggtt accaccagcc tttcttcccc tttgtgtgtg
8461 tggttcaagt gtgcactggg aggaggctga ggcctgtgaa gccaaacaat atgctcctgc
8521 cttgcacctc caataggttt tattattttt tttaaattaa tgaacatatg taatattaat
8581 agttattatt tactggtgca gatggttgac atttttccct attttcctca ctttatggaa
8641 gagttaaaac atttctaaac cagaggacaa aaggggttaa tgttacttta aaattacatt
8701 ctatatatat ataaatatat ataaatatat attaaaatac cagttttttt tctctgggtg
8761 caaagatgtt cattctttta aaaaatgttt aaaaaaaagg aattcgatat caagctgatc
8821 cagatcttat taaagcagaa cttgtttatt gcagcttata atggttacaa ataaagcaat
8881 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
8941 aaactcatca atgtatctta tcatgtctgg tcgactctag actcttccgc ttcctcgctc
9001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
9061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
9121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
9181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
9241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
9301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
9361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
9421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
9481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
9541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
9601 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
9661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
9721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
9781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
9841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
9901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
9961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
10021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
10081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
10141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
10201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
10261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
10321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
10381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
10441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
10501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
10561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
10621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
10681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
10741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
10801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
10861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
10921 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
10981 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
11041 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
11101 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
11161 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
11221 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
11281 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
11341 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
11401 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
11461 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
11521 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
11581 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
11641 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
11701 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
11761 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
11821 aacgacggcc agtgaattgt cgacttctga ggcggaaaga accagctgtg gaatgtgtgt
11881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
11941 ctcaattagt cagcaaggaa agtccccagg ctcccca
//
