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pHUE-vCas9-ipB7RKD载体质粒基本信息

质粒名称: pHUE-vCas9-ipB7RKD
出品公司: Wekwikgene
目录编号: 0002647
产品用途: As a basic vector, construct an Agrobacterium transformation plasmid for homology-directed repair via Agrobacterium-mediated genetic transformation of monocot plants.
存储实验室: Zhen Liang Lab

载体骨架基本信息
载体名称: pHUE411
表达系统: 植物表达
启动子: pOsU3, pZmUbi, p35s
拷贝数: 高拷贝
载体抗性: 卡那霉素
生长温度: 37 ℃
宿主菌: DH5a

插入基因信息
插入基因名称: sgRNA, vCas9-ipB7RKD, Hyg
物种来源: synthetic
密码子优化: 大米表达优化
 

pHUE-vCas9-ipB7RKD质粒图谱载体图谱和pHUE-vCas9-ipB7RKD载体序列质粒序列多克隆位点信息

pHUE-vCas9-ipB7RKD载体图谱

pHUE-vCas9-ipB7RKD质粒载体简介

pHUE-vCas9-ipB7RKD质粒序列载体序列

LOCUS       pHUE-vCas9-ipB7RKD           19804 bp DNA     circular SYN 17-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pHUE-vCas9-ipB7RKD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19804)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..19804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        331..710
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             990..1781
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1933..2008
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2312..4303
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             4322..4387
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4394..4414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4439..8539
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDRGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVAEINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFRLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     variation       4598..4600
                     /label=S55R
     variation       7361..7363
                     /label=R976A
     variation       7442..7444
                     /label=K1003A
     variation       8375..8377
                     /label=T1314R
     RBS             8452..8460
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8540..8587
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    8594..8689
                     /label=32aa linker
     misc_feature    8690..10471
                     /label=piggyBac
     variation       9812..9814
                     /label=K375A
     CDS             10475..10495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11178..11194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11178..11194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11191..11213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11202..11218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11226..11256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11271..11292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11483..12160
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             12228..13253
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13294..13468
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13546..13570
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13995..14789
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14876..15464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15365..15384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15618..15635
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15650..15790
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15716..15735)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15876..15898
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16134..16328
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16394..17467)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17896..18525)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct
      301 aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac
      361 ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag
      421 atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg
      481 ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac
      541 tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat
      601 gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta
      661 tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgagaccaa
      721 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      961 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     1021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1141 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     1441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1741 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     1801 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     1861 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     1921 aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1981 acttgaaaaa gtggcaccga gtcggtgctt ttttttttcg ttttgcattg agttttctcc
     2041 gtcgcatgtt tgcagtttta ttttccgttt tgcattgaaa tttctccgtc tcatgtttgc
     2101 agcgtgttca aaaagtacgc agctgtattt cacttattta cggcgccaca ttttcatgcc
     2161 gtttgtgcca actatcccga gctagtgaat acagcttggc ttcacacaac actggtgacc
     2221 cgctgacctg ctcgtacctc gtaccgtcgt acggcacagc atttggaatt aaagggtgtg
     2281 atcgatactg cttgctgcta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc
     2341 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     2401 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     2461 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     2521 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     2581 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     2641 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     2701 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     2761 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     2821 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     2881 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     2941 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     3001 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     3061 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     3121 acggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg
     3181 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac
     3241 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc
     3301 gctcgtcctc cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     3361 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     3421 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     3481 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     3541 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     3601 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     3661 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta
     3721 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     3781 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     3841 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     3901 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     3961 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     4021 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     4081 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     4141 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     4201 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     4261 cttttgtcga tgctcaccct gttgtttggt gttacttctg cagccctagg cctactagat
     4321 ggattacaag gaccacgacg gggattacaa ggaccacgac attgattaca aggatgatga
     4381 tgacaagatg gctccgaaga agaagaggaa ggttggcatc cacggggtgc cagctgctga
     4441 caagaagtac tcgatcggcc tcgatattgg gactaactct gttggctggg ccgtgatcac
     4501 cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg atcggcattc
     4561 catcaagaag aatctcattg gcgctctcct gttcgacaga ggcgagacgg ctgaggctac
     4621 gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca tctgctacct
     4681 gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc acaggctgga
     4741 ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct tcggcaacat
     4801 tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc ggaagaagct
     4861 cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg cccacatgat
     4921 taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata gcgatgttga
     4981 caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga accccattaa
     5041 tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt ctcggcgcct
     5101 cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg ggaatctcat
     5161 tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg ctgaggacgc
     5221 caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc tggcccagat
     5281 cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg ccatcctcct
     5341 gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag cctccatgat
     5401 caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg tcaggcagca
     5461 gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct acgctgggta
     5521 cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga ttctggagaa
     5581 gatggacggc acggaggagc tgctggtgaa gctcaatcgc gaggacctcc tgaggaagca
     5641 gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc tgcacgctat
     5701 cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga agatcgagaa
     5761 gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca actcccgctt
     5821 cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg aggaggtggt
     5881 cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg acaagaacct
     5941 gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca cagtctacaa
     6001 cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt tcctgtctgg
     6061 ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg tcacggttaa
     6121 gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg agatctctgg
     6181 cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga agatcattaa
     6241 ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca ttgtgctgac
     6301 actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt acgcccatct
     6361 cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct gggggaggct
     6421 gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga tcctcgactt
     6481 cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg atgacagcct
     6541 cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact cgctgcacga
     6601 gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc agaccgtgaa
     6661 ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca tcgtcattga
     6721 gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg agaggatgaa
     6781 gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc acccggtgga
     6841 gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg gccgcgatat
     6901 gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg atcatatcgt
     6961 tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca ggtcggacaa
     7021 gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga tgaagaacta
     7081 ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata acctcacaaa
     7141 ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga ggcagctggt
     7201 cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga tgaacactaa
     7261 gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga agtcaaagct
     7321 cgtctccgac ttcaggaagg atttccagtt ctacaaggtt gcggagatca acaattacca
     7381 ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca agaagtaccc
     7441 agcgctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga ggaagatgat
     7501 cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct actctaacat
     7561 tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga agcgccccct
     7621 catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg atttcgcgac
     7681 cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag aggtccagac
     7741 tggcgggttc tctaaggagt caattctgcc taagcggaac agcgacaagc tcatcgcccg
     7801 caagaaggac tgggatccga agaagtacgg cgggttcgac agccccactg tggcctactc
     7861 ggtcctggtt gtggcgaagg ttgagaaggg caagtccaag aagctcaaga gcgtgaagga
     7921 gctgctgggg atcacgatta tggagcgctc cagcttcgag aagaacccga tcgatttcct
     7981 ggaggcgaag ggctacaagg aggtgaagaa ggacctgatc attaagctcc ccaagtactc
     8041 actcttcgag ctggagaacg gcaggaagcg gatgctggct tccgctggcg agctgcagaa
     8101 ggggaacgag ctggctctgc cgtccaagta tgtgaacttc ctctacctgg cctcccacta
     8161 cgagaagctc aagggcagcc ccgaggacaa cgagcagaag cagctgttcg tcgagcagca
     8221 caagcattac ctcgacgaga tcattgagca gatttccgag ttctccaagc gcgtgatcct
     8281 ggccgacgcg aatctggata aggtcctctc cgcgtacaac aagcaccgcg acaagccaat
     8341 cagggagcag gctgagaata tcattcatct cttccgcctg acgaacctcg gcgcccctgc
     8401 tgctttcaag tacttcgaca caactatcga tcgcaagagg tacacaagca ctaaggaggt
     8461 cctggacgcg accctcatcc accagtcgat taccggcctc tacgagacgc gcatcgacct
     8521 gtctcagctc gggggcgaca agcggccagc ggcgacgaag aaggcggggc aggcgaagaa
     8581 gaagaagctt aagagcggag gatcttccgg aggatctagc ggctccgaga caccaggaac
     8641 atccgaaagc gctacaccag aatctagcgg aggctcttcc ggaggatcta tgggatccag
     8701 tctcgacgac gagcacatct tgtccgcact actgcaatcc gacgacgaac tggtcgggga
     8761 agacagtgat tctgaagtaa gcgaccatgt ctcggaggac gatgtgcagt cggataccga
     8821 agaggccttc attgacgagg ttcatgaggt tcagcccacg tcttcaggat ctgagattct
     8881 tgatgaacaa aatgttattg agcagccggg ctcgagcttg gcttcaaata gaattttaac
     8941 actgcctcaa aggacaatcc gcggcaagaa taagcactgc tggtcaacat ctaaaccaac
     9001 gcgtcgcagc cgcgtgtctg ccttaaatat agtcaggtcc cagcgcgggc ctacaaggat
     9061 gtgcagaaac atttatgatc ctcttctctg cttcaagctg ttcttcaccg acgagataat
     9121 ctccgagatc gtcaagtgga ctaatgctga aatatcgctc aagcgtaggg agtcgatgac
     9181 gtccgcaaca tttagggata cgaatgagga tgaaatttat gcattttttg ggatcctggt
     9241 tatgactgct gtcaggaagg ataaccacat gtcaactgat gacctctttg atcgatcgtt
     9301 gtccatggtg tatgtttccg tcatgagtcg ggacaggttt gatttcctta tacggtgtct
     9361 ccgcatggat gacaagagca tacgaccaac attaagggaa aatgacgtct tcacgcccgt
     9421 tagaaagata tgggacctct tcattcatca gtgcattcag aactacacac ccggcgccca
     9481 cttgaccatt gatgagcaac ttttgggttt tcgcggcaga tgtccgttca gagtgtacat
     9541 ccccaacaag ccatctaagt acggtatcaa aatactgatg atgtgtgact cgggaactaa
     9601 atatatgata aacggcatgc catatcttgg gcggggtact caaaccaatg gtgtgccact
     9661 aggcgagtac tatgtgaaag agctgtcgaa gccggtccac ggaagctgcc gcaacatcac
     9721 ctgcgacaac tggttcactt cgatccctct cgcgaagaac ttgctacagg agccttacaa
     9781 gctcacgatc gttggaactg tggccagcaa cgcacgtgag ataccagaag tactgaaaaa
     9841 ttcacgaagt cggcccgttg gcacttctat gttctgcttt gacggcccgc tcactctcgt
     9901 gtcttataaa ccgaagcctg ccaagatggt ttatctttta tcctcctgtg atgaagatgc
     9961 atccatcaat gaatctacag gaaagccaca aatggtcatg tactacaacc aaacaaaagg
    10021 gggtgtcgac actttgaacc agatgtgcag cgtcatgaca tgttcaagga aaaccaaccg
    10081 gtggccgatg gcccttctct acggcatgat taacattgcc tgtatcaact ccttcatcat
    10141 ttactctcac aatgtgtcat caaagggaga gaaggtacag agtcggaaga agttcatgcg
    10201 taatctctac atgggcctta ccagtagctt catgagaaag agactggagg cgccgaccct
    10261 aaaaaggtac ctcagggata atatctcaaa catcttgcct aaggaagtac caggaacgtc
    10321 agatgattcc accgaggagc ctgtgatgaa gaaaaggacc tactgcacat attgcccgag
    10381 caaaatacgc agaaaggcgt cggcttcatg taagaagtgc aagaaggtga tttgccggga
    10441 gcataatatc gacatgtgcc agtcttgctt cgcgcccaaa aaaaaacgaa aagtgggcat
    10501 tcatggcgtg ccggcggcct gagagctcag agctttcgtt cgtatcatcg gtttcgacaa
    10561 cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca ccagaatcct actgagtttg
    10621 agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact
    10681 gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta
    10741 atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt
    10801 tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat
    10861 gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg
    10921 taccattatg cttattcact aggcaacaaa tatattttca gacctagaaa agctgcaaat
    10981 gttactgaat acaagtatgt cctcttgtgt tttagacatt tatgaacttt cctttatgta
    11041 attttccaga atccttgtca gattctaatc attgctttat aattatagtt atactcatgg
    11101 atttgtagtt gagtatgaaa atatttttta atgcatttta tgacttgcca attgattgac
    11161 aacgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    11221 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    11281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    11341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc
    11401 agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
    11461 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
    11521 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
    11581 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
    11641 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
    11701 caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacactctcg
    11761 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
    11821 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
    11881 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
    11941 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
    12001 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
    12061 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gaccttcctc
    12121 tatataagga agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac
    12181 aaatctatct ctctcgagct ttcgcagatc ccggggggca atgagatatg aaaaagcctg
    12241 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
    12301 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
    12361 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
    12421 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg
    12481 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
    12541 aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg
    12601 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
    12661 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
    12721 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
    12781 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
    12841 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
    12901 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
    12961 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt
    13021 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
    13081 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
    13141 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
    13201 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat
    13261 gccgaccggg atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt
    13321 cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa
    13381 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
    13441 accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat
    13501 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
    13561 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
    13621 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
    13681 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
    13741 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
    13801 tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
    13861 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
    13921 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
    13981 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
    14041 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
    14101 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
    14161 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
    14221 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
    14281 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
    14341 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
    14401 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
    14461 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
    14521 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
    14581 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
    14641 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
    14701 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
    14761 attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac
    14821 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    14881 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    14941 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    15001 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
    15061 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    15121 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    15181 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    15241 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    15301 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    15361 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    15421 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    15481 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    15541 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    15601 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
    15661 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
    15721 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
    15781 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
    15841 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
    15901 ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga
    15961 gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt
    16021 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
    16081 accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac
    16141 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
    16201 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
    16261 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
    16321 tatccacagg aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca
    16381 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
    16441 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
    16501 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
    16561 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
    16621 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
    16681 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
    16741 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
    16801 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
    16861 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
    16921 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
    16981 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
    17041 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
    17101 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
    17161 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
    17221 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
    17281 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
    17341 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
    17401 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
    17461 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac
    17521 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
    17581 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
    17641 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
    17701 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
    17761 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
    17821 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
    17881 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
    17941 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
    18001 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
    18061 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
    18121 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
    18181 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
    18241 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
    18301 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
    18361 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
    18421 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
    18481 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
    18541 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
    18601 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
    18661 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
    18721 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
    18781 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
    18841 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
    18901 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
    18961 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
    19021 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
    19081 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
    19141 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
    19201 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
    19261 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
    19321 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
    19381 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
    19441 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
    19501 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
    19561 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
    19621 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
    19681 tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc
    19741 atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat atccgttatt
    19801 ctaa
//

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