| 质粒名称: | pHybrid-mNG-MNT-mScarlet |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002102 |
| 产品用途: | Green potassium indicator prototype with a potential potassium-binding protein |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pHybrid |
| 表达系统: | 哺乳动物表达、细菌表达 |
| 启动子: | rhaB, CMV, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mNG-MNT, mScarlet |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳动物表达优化 |
买家导航
pHybrid-mNG-MNT-mScarlet载体质粒基本信息
pHybrid-mNG-MNT-mScarlet质粒图谱载体图谱和pHybrid-mNG-MNT-mScarlet载体序列质粒序列多克隆位点信息
pHybrid-mNG-MNT-mScarlet质粒载体简介
pHybrid-mNG-MNT-mScarlet质粒序列载体序列
LOCUS pHybrid-mNG-MNT-mScarlet 6092 bp DNA circular SYN 16-OCT-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHybrid-mNG-MNT-mScarlet
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6092)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported Apr 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6092
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 138..517
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 518..721
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 754..872
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
CDS 913..930
/codon_start=1
/label=6x-HisTag
/translation="HHHHHH"
primer_bind 939..988
/label=mNeo-N-F1
regulatory 961..966
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 967..1400
/label=mNeonGreen
CDS 1402..1410
/codon_start=1
/label=Linker
/translation="EAH"
misc_feature 1411..1878
/label=3-MNT23082.1
primer_bind complement(1861..1898)
/label=3-MNT-mNG-R
CDS 1879..1887
/codon_start=1
/label=Linker
/translation="MYF"
misc_feature 1888..2160
/label=mNeonGreen
primer_bind complement(2139..2185)
/label=mNeo-C-R1
primer_bind 2164..2191
/label=rrnB-F
terminator 2176..2270
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
polyA_signal 2294..2415
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2430..2674
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2525..2660
/label=SV40 ori
/note="SV40 origin of replication"
regulatory 2675..2680
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2681..3376
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
polyA_signal 3381..3428
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3508..3963
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4295..4399
/gene="bla"
/label=AmpR promoter
CDS 4400..5260
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5431..6019
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tcgtttagtg aaccgtcaga tcactagaag cttcaccaca attcagcaaa ttgtgaacat
781 catcacgttc atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt
841 cgcgaattca ggcgcttttt agactggtcg taacccgttt ttggctaaca ggaggaatta
901 accatgggga gccaccatca ccatcaccat gctagcctcg agaattcacg cgtggtacca
961 gccaccatgg tctcaaaggg agaggaggaa aacatggcta gtctgcctgc tacccacgaa
1021 ctgcatattt ttggctccat aaatggcatc gatttcgaca tggtaggcca aggcaccggt
1081 aatccaaacg acggctatga ggagctgaac ctgaagtcca ccatgggcga tctgcagttc
1141 agtccctgga tcctggtgcc gcacattggg tacgggtttc accagtatct tccctatcca
1201 gacgggatgt caccatttca ggctgcaatg gtcgacgggt ccggatacca agtgcaccgc
1261 actatgcagt ttgaggacgg tgcctcactg accgtgaatt acagatacac ctacgaagga
1321 tcacatatca aaggggaagc tcaggtggag ggcactgggt tcccagctga cggccctgtg
1381 atgacaaatt ccctgacagc cgaggcccac ggcctgtttt catttattaa aaacgcgggg
1441 aaaaagctgg gtattggggg ggacgacgac gcgccggaca cggagagtgt tcagaaagaa
1501 ctggcgagcc acgacctggg gacgaaagat gttcaggttg aagttgtgaa cgataaagtg
1561 gttctgaaag gaattgtgaa agatcagaca gcatttgaga aagcagttgt agcagtgggt
1621 aataccctgg gagttagcgc agtggaggca agcgaactga aagttgcaga tacgggtgca
1681 gcaccggcac cggcaaaagc accggtgttt tatacagtga aaaagggaga taatctgtgg
1741 aaaattgcag aagcacatta tggaaaagga aaaggagcaa aacataccgt tatttttgaa
1801 gcaaataaac caatgctgac ccatccggac aaaatttacc cgggtcaggt gctgcgtatc
1861 ccggatctgg atgcagccat gtacttcgct gactggtgcg tatccaagaa gacttgcccc
1921 aatgataaga ctatcgtctc cacatttaag tgggctttta tcaccgacaa cggcaaacgc
1981 taccggtcta ccgctcgcac cacatacaca ttcgccaagc caatggccgc aaattacctc
2041 aaaaatcagc cgatgtatgt ctttcggaaa acagagctga aacattctaa gaccgaactg
2101 aattttaaag agtggcaaaa ggcctttacg gacgtgatgg gcatggacga gctgtataag
2161 taatctagag tcgacccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
2221 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat gcggccgctt
2281 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
2341 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
2401 tgcaataaac aagttaacaa caacaattgg gtgtggaaag tccccaggct ccccagcagg
2461 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2521 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2581 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
2641 aggaggcttt tttggaggcc taggcttttg caaagccacc atggtgagca agggcgaggc
2701 agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga acggccacga
2761 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
2821 gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc ctcagttcat
2881 gtacggctcc agggccttca ccaagcaccc cgccgacatc cccgactact ataagcagtc
2941 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgccgtgac
3001 cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga agctccgcgg
3061 caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct gggaagcgtc
3121 caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaaga tggccctgcg
3181 cctgaaggac ggcggccgct acctggcgga cttcaagacc acctacaagg ccaagaagcc
3241 cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcacct cccacaacga
3301 ggactacacc gtggtggaac agtacgaacg ctccgagggc cgccactcca ccggcggcat
3361 ggacgagctg tacaagtgac cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
3421 gtttgttcgg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
3481 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
3541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
3601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
3661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
3721 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
3781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
3841 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
3901 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
3961 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
4021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
4081 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
4141 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
4201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
4261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
4501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
4561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
4621 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
4681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
4741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
4801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
4861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
4921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
4981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
5041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
5101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
5161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
5221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
5281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
5341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
5401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
5461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
5521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
5581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
5881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
5941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
6001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
6061 ctggcctttt gctcacatgg ctcgacagat ct
//
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