| 质粒名称: | pYK63 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000786 |
| 产品用途: | Mammalian PhCMV-driven constitutive SEAP expression vector containing 16 tandem MS2-box repeats in the 3’UTR (PhCMV-SEAP-(MS2-box)16-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: | SEAP-(MS2-box)16 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK63
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK63载体质粒基本信息
pYK63质粒图谱载体图谱和pYK63载体序列质粒序列多克隆位点信息
pYK63质粒载体简介
pYK63质粒序列载体序列
LOCUS pYK63 7819 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7819)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7819
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..917
/label=oYK55-SEAP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 900..927
/label=oDJ273-1
misc_feature 901..2460
/label=SEAP
primer_bind complement(2439..2460)
/label=oDJ273-2
primer_bind complement(2441..2475)
/label=pYK62-SEAP-R
primer_bind complement(2441..2460)
/label=oYK55-SEAP-R
primer_bind complement(2444..2460)
/label=Oscl31-4
primer_bind complement(2444..2460)
/label=oSCL083
polyA_signal 2452..2460
/label=SV40 late polyA
misc_feature 2469..2473
/label=New Feature
misc_feature 2469..2473
/label=New Feature
misc_feature 2492..2495
/label=linker
primer_bind complement(2497..2511)
/label=Oscl28-2
primer_bind complement(2498..2511)
/label=8x R
misc_feature 2512..2524
/label=linker
misc_feature 2530..2548
/label=MS2 box
misc_feature 2554..2563
/label=linker
misc_feature 2569..2587
/label=MS2 box
primer_bind complement(2580..2600)
/label=oYK55-SEAP-R
misc_feature 2593..2602
/label=linker
misc_feature 2593..2602
/label=linker
misc_feature 2601..2631
/label=hairpin
misc_feature 2607..2625
/label=MS2 box
misc_feature 2630..2642
/label=linker
misc_feature 2641..2671
/label=hairpin
misc_feature 2647..2665
/label=MS2 box
misc_feature 2675..2680
/label=linker
misc_feature 2689..2704
/label=linker
misc_feature 2710..2713
/label=linker
primer_bind complement(2715..2729)
/label=Oscl28-2
primer_bind complement(2716..2729)
/label=8x R
misc_feature 2730..2742
/label=linker
misc_feature 2748..2766
/label=MS2 box
misc_feature 2772..2781
/label=linker
misc_feature 2787..2805
/label=MS2 box
primer_bind complement(2798..2818)
/label=oYK55-SEAP-R
misc_feature 2811..2820
/label=linker
misc_feature 2811..2820
/label=linker
misc_feature 2819..2849
/label=hairpin
misc_feature 2825..2843
/label=MS2 box
misc_feature 2848..2860
/label=linker
misc_feature 2859..2889
/label=hairpin
misc_feature 2865..2883
/label=MS2 box
misc_feature 2893..2898
/label=linker
misc_feature 2907..2922
/label=linker
misc_feature 2949..2961
/label=linker
misc_feature 2967..2985
/label=MS2 box
misc_feature 2991..3000
/label=linker
misc_feature 3006..3024
/label=MS2 box
primer_bind complement(3017..3037)
/label=oYK55-SEAP-R
misc_feature 3030..3039
/label=linker
misc_feature 3030..3039
/label=linker
misc_feature 3038..3068
/label=hairpin
misc_feature 3044..3062
/label=MS2 box
misc_feature 3067..3079
/label=linker
misc_feature 3078..3108
/label=hairpin
misc_feature 3084..3102
/label=MS2 box
misc_feature 3112..3117
/label=linker
misc_feature 3126..3141
/label=linker
misc_feature 3147..3159
/label=linker
misc_feature 3165..3183
/label=MS2 box
misc_feature 3189..3198
/label=linker
misc_feature 3204..3222
/label=MS2 box
primer_bind complement(3215..3235)
/label=oYK55-SEAP-R
misc_feature 3228..3237
/label=linker
misc_feature 3228..3237
/label=linker
misc_feature 3236..3266
/label=hairpin
misc_feature 3242..3260
/label=MS2 box
misc_feature 3265..3277
/label=linker
misc_feature 3276..3306
/label=hairpin
misc_feature 3282..3300
/label=MS2 box
misc_feature 3310..3315
/label=linker
misc_feature 3324..3339
/label=linker
terminator 3413..3640
/label=bGH PA
misc_feature 3686..4114
/label=f1 origin
misc_feature 4119..4462
/label=SV40 early promoter and origin
misc_feature 4308..4385
/label=SV40 ORI
CDS 4527..5318
/label=Kan/neoR
misc_feature 5492..5622
/label=SV40 early polyadenylation signal
primer_bind complement(5658..5678)
/label=M13-rev
misc_binding complement(5684..5706)
/label=LacO
promoter complement(5711..5740)
/label=lac
misc_feature complement(6005..6678)
/label=pUC origin
CDS complement(6826..7485)
/label=AmpR
promoter complement(7725..7753)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgctgctgc tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca
961 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
1021 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
1081 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
1141 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
1201 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
1261 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
1321 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
1381 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
1441 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
1501 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
1561 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
1621 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
1681 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
1741 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
1801 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
1861 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
1921 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
1981 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
2041 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
2101 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
2161 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
2221 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
2281 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
2341 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
2401 gacgccgcgc acccgggtta ctctagagtc ggggcggccg gccgcttcga gcagacatga
2461 aaaccccaag cttggtaccg agctcggatc caacttgaac cggtaagaat tgcaacaaac
2521 aaatcaccac cgtgaggatc acccacggtg gtgaaagcct tagcctagcc gtgaggatca
2581 cccacggtag gcacaagaaa ttctacccgt gaggatcacc cacgggtaga atcacaaaga
2641 ctacagccgt gaggatcacc cacggctgta gactcaaact taaaaaggat atcttcagca
2701 aactgatcca acttgaaccg gtaagaattg caacaaacaa atcaccaccg tgaggatcac
2761 ccacggtggt gaaagcctta gcctagccgt gaggatcacc cacggtaggc acaagaaatt
2821 ctacccgtga ggatcaccca cgggtagaat cacaaagact acagccgtga ggatcaccca
2881 cggctgtaga ctcaaactta aaaaggatat cttcagcaaa ctgatcggag acgagtccgt
2941 ctcaaattgc aacaaacaaa tcaccaccgt gaggatcacc cacggtggtg aaagccttag
3001 cctagccgtg aggatcaccc acggtaggca caagaaattc tacccgtgag gatcacccac
3061 gggtagaatc acaaagacta cagccgtgag gatcacccac ggctgtagac tcaaacttaa
3121 aaaggatatc ttcagcaaac tgaattgcaa caaacaaatc accaccgtga ggatcaccca
3181 cggtggtgaa agccttagcc tagccgtgag gatcacccac ggtaggcaca agaaattcta
3241 cccgtgagga tcacccacgg gtagaatcac aaagactaca gccgtgagga tcacccacgg
3301 ctgtagactc aaacttaaaa aggatatctt cagcaaactg aattctgcag atatccagca
3361 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
3421 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
3481 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
3541 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
3601 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
3661 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
3721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
3781 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3841 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3901 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3961 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
4021 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
4081 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
4141 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
4201 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4261 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
4321 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
4381 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
4441 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
4501 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
4561 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
4621 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
4681 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
4741 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
4801 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
4861 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
4921 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
4981 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
5041 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
5101 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
5161 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
5221 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
5281 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
5341 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
5401 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
5461 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
5521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
5581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
5641 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
5701 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
5761 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
5821 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
5881 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
5941 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
6001 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
6061 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
6121 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
6181 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
6241 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
6301 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
6361 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
6421 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
6481 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
6541 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
6601 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
6661 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
6721 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
6781 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
6841 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
6901 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
6961 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
7021 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
7081 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
7141 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
7201 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
7261 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
7321 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
7381 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
7441 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
7501 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
7561 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
7621 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
7681 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
7741 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
7801 aaaagtgcca cctgacgtc
//
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