| 质粒名称: | pJBEI-6409 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 47048 |
| 存储实验室: | Taek Soon Lee |
|
载体骨架基本信息 |
|
| 载体名称: | pBbA5c |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 3500 bp |
| 完整质粒大小: | 14932 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，合成生物学；布格瑞克 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene |
| 物种来源: | S. cerevisiae (budding yeast); Mentha spicata, Abies grandis and Staphylococcus aureus |
| 插入大小: | 11500 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | PlacUV5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI, BglII (未破坏) |
| 3' 克隆位点: | BamHI, XhoI (未破坏) |
| 5' 测序引物 | F5 |
| 3' 测序引物 | R1 |
|
文献引用方法 |
|
| 材料和方法部分: | pJBEI-6409 was a gift from Taek Soon Lee (Addgene plasmid # 47048 ; http://n2t.net/addgene:47048 ; RRID:Addgene_47048) |
| 参考文献部分: | Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ, Lee TS. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. 10.1016/j.ymben.2013.05.004 PubMed 23727191 |
买家导航
pJBEI-6409载体 pJBEI6409质粒基本信息
pJBEI-6409质粒图谱 pJBEI6409载体图谱和pJBEI-6409载体序列 pJBEI6409质粒序列多克隆位点信息
pJBEI-6409质粒 pJBEI6409载体简介
pJBEI-6409质粒序列 pJBEI6409载体序列
LOCUS pJBEI-6409 14932 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION BglBrick plasmid
(=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV
pathway enzymes to produce limonene from glucose in E. coli.
ACCESSION .
VERSION .
KEYWORDS pJBEI-6409
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14932)
AUTHORS Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold
CJ, Lee TS
TITLE Metabolic engineering of Escherichia coli for limonene and perillyl
alcohol production.
JOURNAL Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
PUBMED 23727191
REFERENCE 2 (bases 1 to 14932)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14932
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(49..1131)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 1093..1112
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(1132..1209)
/gene="lacI"
/label=lacI promoter
primer_bind 1415..1434
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 1520..1550
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind 1558..1574
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS complement(2309..2320)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
terminator 5324..5395
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5411..5438
/label=T7Te terminator
/note="phage T7 early transcription terminator"
promoter 5650..5679
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 5687..5703
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 8726..8737
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 10432..10461
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 10469..10485
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
terminator 13097..13168
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 13184..13211
/label=T7Te terminator
/note="phage T7 early transcription terminator"
primer_bind complement(13239..13256)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(13373..13918)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(13453..13472)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 14032..14126
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(14147..14806)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 14721..14740
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(14807..14909)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
ORIGIN
1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
1561 tgagcggata acaatttcag aattcaaaag atcttaggag gaatataaaa tgaaaaattg
1621 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
1681 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
1741 ttcacaacac gttgatgaag tgattatggg taacgtgtta caagccgggc tggggcaaaa
1801 tccggcgcgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg gattcacggt
1861 caataaagta tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg ccattcaggc
1921 aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag ccccctactt
1981 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
2041 cctgcgcgat ggcctgatgt gcgccaccca tggttatcat atggggatta ccgccgaaaa
2101 cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc tacattcaca
2161 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
2221 tgttgtcact cgaaagaaaa ccttcgtctt cagtcaagac gagttcccga aagcgaactc
2281 aacggctgaa gcgttaggtg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
2341 tgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg aagaatctgc
2401 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
2461 gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt tacaactggc
2521 ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg ctgcacagtt
2581 ccttgccgtt gggaaaaacc tgggctttga ttctgagaaa gtgaatgtca acggcggggc
2641 catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca cactattaca
2701 tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctgtgcattg gcggcggtca
2761 gggaattgcg atggtgattg aacggttgaa ttgaggatct tgaattaagg aggacagcta
2821 aatgacaata ggtatcgata aaataaactt ttacgttcca aagtactatg tagacatggc
2881 taaattagca gaagcacgcc aagtagaccc aaacaaattt ttaattggaa ttggtcaaac
2941 tgaaatggct gttagtcctg taaaccaaga catcgtttca atgggcgcta acgctgctaa
3001 ggacattata acagacgaag acaaaaagaa aattggtatg gtaattgtgg caactgaatc
3061 agcagttgat gctgctaaag cagccgctgt tcaaattcac aacttattag gtattcaacc
3121 ttttgcacgc tgctttgaaa tgaaagaagc ttgttatgct gcaacaccag caattcaatt
3181 agctaaagat tatttagcaa ctagaccgaa tgaaaaagta ttagttattg ctacagatac
3241 agcacgttat ggattgaact caggcggcga gccaacacaa ggtgctggcg cagttgcgat
3301 ggttattgca cataatccaa gcattttggc attaaatgaa gatgctgttg cttacactga
3361 agacgtttat gatttctggc gtccaactgg acataaatat ccattagttg atggtgcatt
3421 atctaaagat gcttatatcc gctcattcca acaaagctgg aatgaatacg caaaacgtca
3481 aggtaagtcg ctagctgact tcgcatctct atgcttccat gttccattta caaaaatggg
3541 taaaaaggca ttagagtcaa tcattgataa cgctgatgaa acaactcaag agcgtttacg
3601 ttcaggatat gaagatgctg tagattataa ccgttatgtc ggtaatattt atactggatc
3661 attatattta agcctaatat cattacttga aaatcgagat ttacaagctg gtgaaacaat
3721 cggtttattc agttatggct caggttcagt tggtgaattt tatagtgcga cattagttga
3781 aggctacaaa gatcatttag atcaagctgc acataaagca ttattaaata accgtactga
3841 agtatctgtt gatgcatatg aaacattctt caaacgtttt gatgacgttg aatttgacga
3901 agaacaagat gctgttcatg aagatcgtca tattttctac ttatcaaata ttgaaaataa
3961 cgttcgcgaa tatcacagac cagagtaatt aggatctatt caggaaacag accatgtcca
4021 tgcaaagttt agataagaat tttcgacatt tatctcgtaa agaaaagtta caacaattgg
4081 ttgataagca atggttatca gaagaacaat tcgacatttt actgaatcat ccattaatcg
4141 atgaagaagt agccaatagt ttaattgaaa atgtcatcgc gcaaggtgca ttacccgttg
4201 gattattacc gaatatcatt gtggacgata aggcatatgt tgtacctatg atggtggaag
4261 agccttcagt tgtcgctgca gctagttatg gtgcaaagct agtgaatcag actggcggat
4321 ttaaaacggt atcttctgaa cgtattatga taggtcaaat cgtctttgat ggcgttgacg
4381 atactgaaaa attatcagca gacattaaag ctttagaaaa gcaaattcat aaaattgcgg
4441 atgaggcata tccttctatt aaagcgcgtg gtggtggtta ccaacgtata gcgattgata
4501 catttcctga gcaacagtta ctatctttaa aagtatttgt tgatacgaaa gatgctatgg
4561 gcgctaatat gcttaatacg attttagagg ccataactgc atttttaaaa aatgaatttc
4621 cgcaaagcga cattttaatg agtattttat ccaatcatgc aacagcgtcc gttgttaaag
4681 ttcaaggcga aattgatgtt aaagatttag caaggggcga gagaactgga gaagaggttg
4741 ccaaacgaat ggaacgtgct tctgtattgg cccaagtaga tattcatcgt gcagcaacac
4801 ataataaagg tgttatgaat ggcatacatg ctgttgtttt agcaacagga aatgatacgc
4861 gtggtgcaga agcaagtgcg catgcatacg cgagtcgtga cggacagtat cgtggtattg
4921 ctacatggcg ttacgatcaa gatcgtcaac gattgattgg tacaattgaa gtgcctatga
4981 cattggcaat cgttggcggt ggtacaaaag tattaccaat tgctaaagct tcattagagc
5041 tactaaatgt agagtcagca caagaattag gtcatgtagt tgctgccgtt ggtttagcgc
5101 aaaactttgc agcatgtcgc gcgcttgtgt cagaaggtat tcaacaaggt catatgagtt
5161 tacaatataa atcattagct atcgttgtag gggcaaaagg tgatgaaatt gctaaagtag
5221 ctgaagcttt gaaaaaagaa ccccgtgcaa atacacaagc agcggaacat attttacaag
5281 aaattagaca acaataagga tctttttaag gatctccagg catcaaataa aacgaaaggc
5341 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
5401 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatagcgaat tgatctggtt
5461 tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga
5521 agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca
5581 ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga
5641 aatgagctgt tgacaattaa tcatccggct cgtataatgt gtggaattgt gagcggataa
5701 caatttcagg atctaggagg aaataaccat gtctctgcca ttcctgacgt ctgcgccagg
5761 taaggtgatc atcttcggcg agcactctgc ggtgtacaat aagccggccg tcgccgcctc
5821 tgtgtctgcg ttacgcacct acctgctgat cagcgaatct tctgcaccgg acacgatcga
5881 gctggacttt ccggacatca gcttcaacca caagtggagc atcaacgact tcaacgcgat
5941 cacggaggac caggtgaaca gccaaaagct ggccaaagcc cagcaagcaa ccgacggtct
6001 gtctcaggag ctggtgtctc tgctggaccc gctgttagcg cagttaagcg agagcttcca
6061 ttaccacgcc gcgttctgct tcctgtacat gttcgtttgc ctgtgcccgc acgcaaagaa
6121 catcaagttc agcctgaaga gcacgctgcc gattggcgca ggcttaggct ctagcgcatc
6181 tatcagcgtg agcctggcgc tggcgatggc ctatctgggt ggcctgattg gcagcaacga
6241 cctggagaaa ctgagcgaaa acgacaagca catcgtgaac cagtgggcct ttatcggcga
6301 gaagtgcatt catggcaccc cgagcggcat tgacaacgca gttgccacgt atggcaacgc
6361 cctgctgttc gagaaagaca gccacaacgg cacgatcaac acgaacaact tcaagttcct
6421 ggacgacttc ccggcgatcc cgatgattct gacctacacc cgtatcccac gcagcaccaa
6481 ggatttagtc gcccgcgtgc gtgttttagt caccgaaaag ttcccggagg tgatgaagcc
6541 gatcctggac gcgatgggcg agtgcgcgct gcagggtctg gagatcatga ccaagctgag
6601 caagtgcaag ggcaccgacg atgaggcggt ggagaccaac aatgagctgt acgagcagct
6661 gctggagctg atccgtatca atcacggcct gctggtctct atcggtgtgt ctcacccggg
6721 cctggaactg atcaaaaacc tgagcgacga cctgcgcatt ggctctacga aattaacggg
6781 tgcaggtggc ggtggctgct ctttaacgct gctgcgccgt gacattacgc aggagcaaat
6841 cgacagcttc aagaagaagc tgcaggacga cttcagctac gagacgttcg agacggacct
6901 gggcggcacg ggctgttgcc tgctgagcgc caaaaatctg aacaaggacc tgaagatcaa
6961 aagcctggtg ttccagctgt tcgaaaacaa gacgaccacg aagcagcaga tcgacgacct
7021 gttactgccg ggtaacacca atctgccgtg gacgtcttaa ggatctagga gggagatcat
7081 atgagcgaat tacgtgcatt cagcgcgcca ggtaaggcac tgctggccgg tggctacctg
7141 gtgttagaca ccaagtacga ggcgttcgtc gtcggcttat ctgcccgtat gcatgcagtt
7201 gcccacccgt atggtagcct gcagggctct gacaagttcg aagtgcgtgt gaagagcaag
7261 cagttcaagg acggcgagtg gctgtaccac attagcccaa agagcggctt catcccggtt
7321 agcattggtg gcagcaagaa cccatttatc gagaaggtca ttgccaacgt cttcagctac
7381 ttcaagccga atatggacga ttactgcaac cgcaacctgt tcgtcatcga cattttcagc
7441 gacgacgcgt accacagcca agaggactct gttacggagc atcgtggtaa ccgccgcctg
7501 agcttccaca gccatcgcat tgaggaggtg ccgaagacgg gtctgggttc tagcgccggt
7561 ttagttaccg tcttaacgac ggcgttagcg agcttcttcg tgagcgacct ggagaacaac
7621 gtggacaagt accgcgaagt gattcataac ctggcgcagg tggcacattg tcaggcccaa
7681 ggtaagattg gctctggttt tgatgtggca gcggccgcct atggctctat ccgctatcgc
7741 cgctttccgc cggccctgat cagcaatctg ccggacatcg gctctgcgac gtatggtagc
7801 aaactggcgc atctggtgga cgaagaagac tggaacatca ccattaagtc taatcacctg
7861 ccgagcggct taacgttatg gatgggcgat atcaagaacg gcagcgaaac ggttaagctg
7921 gtgcagaaag tgaaaaactg gtacgacagc cacatgccgg aaagcctgaa gatttacacg
7981 gagctggacc acgccaatag ccgtttcatg gatggtctga gcaagctgga ccgcctgcac
8041 gaaacccacg acgactacag cgaccaaatc ttcgagagcc tggagcgcaa tgactgcacc
8101 tgccagaagt acccggagat cacggaggtc cgcgatgccg tggcaacgat tcgccgtagc
8161 ttccgcaaaa ttacgaagga gagcggcgcg gatatcgaac caccggtcca gacgtctctg
8221 ctggacgact gtcaaacctt aaagggcgtg ttaacgtgcc tgattccggg cgcgggtggt
8281 tacgacgcca ttgccgtcat cacgaaacag gacgtcgatc tgcgcgcaca aacggccaac
8341 gacaaacgtt tcagcaaagt ccaatggctg gatgttacgc aggccgactg gggtgttcgc
8401 aaggagaagg acccggaaac gtatctggat aagtgaggat ctaggaggat tatgagatga
8461 ccgtttacac agcatccgtt accgcacccg tcaacatcgc aacccttaag tattggggga
8521 aaagggacac gaagttgaat ctgcccacca attcgtccat atcagtgact ttatcgcaag
8581 atgacctcag aacgttgacc tctgcggcta ctgcacctga gtttgaacgc gacactttgt
8641 ggttaaatgg agaaccacac agcatcgaca atgaaagaac tcaaaattgt ctgcgcgacc
8701 tacgccaatt aagaaaggaa atggaatcga aggacgcctc attgcccaca ttatctcaat
8761 ggaaactcca cattgtctcc gaaaataact ttcctacagc agctggttta gcttcctccg
8821 ctgctggctt tgctgcattg gtctctgcaa ttgctaagtt ataccaatta ccacagtcaa
8881 cttcagaaat atctagaata gcaagaaagg ggtctggttc agcttgtaga tcgttgtttg
8941 gcggatacgt ggcctgggaa atgggaaaag ctgaagatgg tcatgattcc atggcagtac
9001 aaatcgcaga cagctctgac tggcctcaga tgaaagcttg tgtcctagtt gtcagcgata
9061 ttaaaaagga tgtgagttcc actcagggta tgcaattgac cgtggcaacc tccgaactat
9121 ttaaagaaag aattgaacat gtcgtaccaa agagatttga agtcatgcgt aaagccattg
9181 ttgaaaaaga tttcgccacc tttgcaaagg aaacaatgat ggattccaac tctttccatg
9241 ccacatgttt ggactctttc cctccaatat tctacatgaa tgacacttcc aagcgtatca
9301 tcagttggtg ccacaccatt aatcagtttt acggagaaac aatcgttgca tacacgtttg
9361 atgcaggtcc aaatgctgtg ttgtactact tagctgaaaa tgagtcgaaa ctctttgcat
9421 ttatctataa attgtttggc tctgttcctg gatgggacaa gaaatttact actgagcagc
9481 ttgaggcttt caaccatcaa tttgaatcat ctaactttac tgcacgtgaa ttggatcttg
9541 agttgcaaaa ggatgttgcc agagtgattt taactcaagt cggttcaggc ccacaagaaa
9601 caaacgaatc tttgattgac gcaaagactg gtctaccaaa ggaataagga tctaggaggt
9661 aatgataatg caaacggaac acgtcatttt attgaatgca cagggagttc ccacgggtac
9721 gctggaaaag tatgccgcac acacggcaga cacccgctta catctcgcgt tctccagttg
9781 gctgtttaat gccaaaggac aattattagt tacccgccgc gcactgagca aaaaagcatg
9841 gcctggcgtg tggactaact cggtttgtgg gcacccacaa ctgggagaaa gcaacgaaga
9901 cgcagtgatc cgccgttgcc gttatgagct tggcgtggaa attacgcctc ctgaatctat
9961 ctatcctgac tttcgctacc gcgccaccga tccgagtggc attgtggaaa atgaagtgtg
10021 tccggtattt gccgcacgca ccactagtgc gttacagatc aatgatgatg aagtgatgga
10081 ttatcaatgg tgtgatttag cagatgtatt acacggtatt gatgccacgc cgtgggcgtt
10141 cagtccgtgg atggtgatgc aggcgacaaa tcgcgaagcc agaaaacgat tatctgcatt
10201 tacccagctt aaataaggat ctcgcaaaaa accccgcttc ggcggggttt tttcgccgac
10261 tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg
10321 caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg
10381 ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt
10441 aatcatccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca ggatctttta
10501 agaaggagat atacatggaa tttgacttca acaaatacat ggactccaaa gcgatgacgg
10561 taaatgaagc actgaacaaa gcgatccctc tgcgttatcc gcagaaaatc tacgaaagca
10621 tgcgttacag cctgctggca ggcggcaagc gtgttcgtcc ggttctgtgt attgccgcat
10681 gtgaactggt aggtggtacc gaagaactgg cgatcccgac cgcgtgcgca attgaaatga
10741 tccacacgat gtccctgatg cacgatgatc tgccgtgtat cgacaacgac gatctgcgtc
10801 gcggtaaacc gactaaccac aaaattttcg gtgaggatac cgcagtgact gctggtaacg
10861 cactgcactc ttacgccttc gagcatatcg cggtttctac ttctaaaacc gttggtgctg
10921 accgcatcct gcgtatggtg tccgagctgg gtcgtgctac tggctctgaa ggtgttatgg
10981 gtggtcagat ggtagacatc gcatccgaag gcgatccgtc tatcgacctg cagaccctgg
11041 aatggattca catccacaaa accgcaatgc tgctggaatg ctccgttgtt tgcggtgcaa
11101 tcattggcgg tgccagcgaa atcgtaatcg aacgtgcccg tcgctacgcc cgctgtgttg
11161 gtctgctgtt ccaggtagtt gatgacattc tggacgtaac taaaagcagc gacgaactgg
11221 gtaagactgc gggcaaggac ctgatctctg ataaagccac ctacccaaag ctgatgggtc
11281 tggaaaaggc caaggagttc tccgatgaac tgctgaaccg tgcgaagggt gaactgtcct
11341 gcttcgaccc agttaaagcc gctccgctgc tgggcctggc agactacgtg gcatttcgtc
11401 agaattaagg atcttttaag aaggagatat acatgcgtcg cagtggtaat tacaacccgt
11461 cacgttggga tgtgaatttt atccaatccc tgctgagcga ttacaaagag gacaaacacg
11521 tcattcgggc aagcgaactg gttacactgg ttaaaatgga actggaaaaa gaaacagacc
11581 aaattcgcca attagagctg attgacgact tacagcgcat ggggctgagc gatcactttc
11641 agaatgagtt caaagagatt ctgtcatcta tctatctgga ccaccattac tataaaaacc
11701 cttttccgaa agaggaacgt gatctgtata gcacaagcct ggcctttcgg ttactgcgtg
11761 agcatggctt ccaagtagca caagaggtat tcgatagctt caagaatgaa gagggcgagt
11821 tcaaagaatc actgtccgat gatactcgtg gcttattaca actgtatgaa gcgagctttc
11881 tgctgaccga gggggagacg actctggaaa gcgcacggga atttgcgacc aaattcttag
11941 aagagaaagt aaatgaaggc ggcgtagatg gcgatctgtt aacccgcatc gcgtattctc
12001 tggacattcc gttacactgg cgtattaaac ggccaaacgc gccggtgtgg attgagtggt
12061 atcgcaaacg tccagacatg aacccggtgg tgctggaatt agcaatttta gacttaaaca
12121 tcgtgcaggc tcaatttcag gaagaactga aagaatcttt tcgctggtgg cgcaacaccg
12181 gcttcgttga aaaattaccg tttgcgcgcg atcgtctggt tgaatgttat ttctggaata
12241 ccggcattat cgaaccgcgc cagcacgcga gcgcccgtat tatgatgggt aaggtcaatg
12301 cgctgattac tgtcattgac gatatttacg atgtatacgg cactctggaa gaactggagc
12361 agtttacaga tttaatccgc cggtgggaca tcaatagcat cgaccagctg ccagattaca
12421 tgcagctgtg ttttctggcg ctgaataact ttgtggacga cacctcttat gacgtcatga
12481 aagaaaaagg cgtgaatgtt atcccttatc tgcgtcagag ttgggtggat ctggccgata
12541 aatatatggt tgaggcacgc tggttctacg gtgggcacaa accgagttta gaagaatatc
12601 tggagaactc atggcagagt atcagtgggc catgcatgct gacgcatatc ttctttcgcg
12661 tcacggattc atttaccaag gagacggtcg attccttata taaataccat gacctggttc
12721 gttggtcatc tttcgtcctg cggctggcgg acgatctggg tacgtctgtg gaagaggtgt
12781 ctcgtgggga tgttccgaaa tccttacagt gctacatgtc cgattataac gcaagtgaag
12841 ctgaagctcg caaacatgtg aagtggctga tcgccgaagt gtggaagaag atgaacgctg
12901 aacgtgtttc caaggatagt ccgtttggta aggattttat tggttgcgcc gttgatctgg
12961 gtcgtatggc ccagctgatg tatcataacg gtgatggtca tgggacccag catcctatta
13021 ttcatcagca gatgacgcgt accctgtttg cacctttcgc ctaaggatcc aaactcgagt
13081 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
13141 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
13201 gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc
13261 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
13321 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
13381 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
13441 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
13501 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
13561 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
13621 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
13681 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
13741 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
13801 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
13861 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
13921 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
13981 tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc
14041 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
14101 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
14161 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
14221 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
14281 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
14341 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
14401 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
14461 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
14521 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
14581 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
14641 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
14701 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
14761 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
14821 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
14881 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccagata tc
//
