| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-SBF1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001874 |
| 产品用途: | 5' AAVLINK plasmid for SBF1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SBF1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-SBF1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-SBF1载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-SBF1质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-SBF1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-SBF1质粒载体简介
pAAVLINK-EF1alpha-HA-5'-SBF1质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-SBF1 6976 bp DNA circular SYN 25-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6976)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6976
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4116
/codon_start=1
/gene="SBF1"
/gene_synonym="CMT4B3; DENND7A; MTMR5"
/product="myotubularin-related protein 5 isoform 1"
/label=SBF1
/note="isoform 1 is encoded by transcript variant 1;
myotubularin-related protein 5; DENN/MADD domain containing
7A; inactive phosphatidylinositol 3-phosphatase 5"
/db_xref="GI:239735519"
/db_xref="CCDS:CCDS14091.2"
/db_xref="GeneID:6305"
/db_xref="HGNC:HGNC:10542"
/db_xref="MIM:603560"
/protein_id="NP_002963.2"
/translation="ARLADYFVLVAFGPHPRGSGEGQGQILQRFPEKDWEDNPFPQGIE
LFCQPSGWQLCPERNPPTFFVAVLTNINSERHYCACLTFWEPAEPSQETTRVEDATERE
EEGDEGGQTHLSPTAPAPSAQLFAPKTLVLVSRLDHTEVFRNSLGLIYAIHVEGLNVCL
ENVIGNLLTCTVPLAGGSQRTISLGAGDRQVIQTPLADSLPVSRCSVALLFRQLGITNV
LSLFCAALTEHKVLFLSRSYQRLADACRGLLALLFPLRYSFTYVPILPAQLLEVLSTPT
PFIIGVNAAFQAETQELLDVIVADLDGGTVTIPECVHIPPLPEPLQSQTHSVLSMVLDP
ELELADLAFPPPTTSTSSLKMQDKELRAVFLRLFAQLLQGYRWCLHVVRIHPEPVIRFH
KAAFLGQRGLVEDDFLMKVLEGMAFAGFVSERGVPYRPTDLFDELVAHEVARMRADENH
PQRVLRHVQELAEQLYKNENPYPAVAMHKVQRPGESSHLRRVPRPFPRLDEGTVQWIVD
QAAAKMQGAPPAVKAERRTTVPSGPPMTAILERCSGLHVNSARRLEVVRNCISYVFEGK
MLEAKKLLPAVLRALKGRAARRCLAQELHLHVQQNRAVLDHQQFDFVVRMMNCCLQDCT
SLDEHGIAAALLPLVTAFCRKLSPGVTQFAYSCVQEHVVWSTPQFWEAMFYGDVQTHIR
ALYLEPTEDLAPAQEVGEAPSQEDERSALDVASEQRRLWPTLSREKQQELVQKEESTVF
SQAIHYANRMSYLLLPLDSSKSRLLRERAGLGDLESASNSLVTNSMAGSVAESYDTESG
FEDAETCDVAGAVVRFINRFVDKVCTESGVTSDHLKGLHVMVPDIVQMHIETLEAVQRE
SRRLPPIQKPKLLRPRLLPGEECVLDGLRVYLLPDGREEGAGGSAGGPALLPAEGAVFL
TTYRVIFTGMPTDPLVGEQVVVRSFPVAALTKEKRISVQTPVDQLLQDGLQLRSCTFQL
LKMAFDEEVGSDSAELFRKQLHKLRYPPDIRATFAFTLGSAHTPGRPPRVTKDKGPSLR
TLSRNLVKNAKKTIGRQHVTRKKYNPPSWEHRGQPPPEDQEDEISVSEELEPSTLTPSS
ALKPSDRMTMSSLVE"
misc_feature 1501..1506
/label=SDS
misc_feature 1815..1820
/label=SDS
misc_feature 2348..2353
/label=SDS
misc_feature 2871..2876
/label=SDS
misc_feature 2895..2900
/label=SDS
misc_feature 2988..2993
/label=SDS
misc_feature 3043..3048
/label=SDS
misc_feature 3046..3051
/label=SDS
misc_feature 3474..3479
/label=SDS
misc_feature 3735..3740
/label=SDS
misc_feature 4011..4016
/label=SDS
intron 4117..4157
/label=Splicing Donor
misc_feature 4161..4194
/label=lox2272-JT1571
repeat_region 4239..4379
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4239..4368
/label=AAV2 ITR
repeat_region 4240..4380
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4454..4909
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5191..5295
/gene="bla"
/label=AmpR promoter
CDS 5296..6156
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6327..6915
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctgc gcggctcgcg gactacttcg tgctggtggc
781 gttcgggccg cacccgcgcg ggagtgggga aggccagggc cagattctgc agcgcttccc
841 agagaaggac tgggaggaca acccattccc ccagggcatc gagctgtttt gccagcccag
901 cgggtggcag ctgtgtcccg agaggaatcc accgaccttc tttgttgctg tcctcaccaa
961 catcaactcc gagcgccact actgcgcctg cttgaccttc tgggagccag cggagccttc
1021 acaggaaacg acgcgcgtgg aggatgccac agagagggag gaagaggggg atgagggagg
1081 ccagacccac ctgtctccca cagcacctgc cccatctgcc cagctgtttg caccgaagac
1141 gctggtactg gtgtcgcgac tcgaccacac ggaggtgttc aggaacagcc ttggcctcat
1201 ctatgccatc cacgtggagg gcctgaatgt gtgcctggag aacgtgattg ggaacctgct
1261 gacgtgcact gtgcccctgg ctgggggctc gcagaggacg atctctttgg gggctggtga
1321 ccggcaggtc atccagactc cactggctga ctcgctgccc gtcagccgct gcagcgtggc
1381 cctgctcttc cgccagctag gcatcaccaa cgtgctgtct ttgttctgtg ccgccctcac
1441 ggagcacaag gttctcttcc tgtcccggag ctaccagcgg ctcgccgatg cctgtagggg
1501 cctcctggca ctgctgtttc ctctcagata cagcttcacc tatgtgccca tcctgccggc
1561 tcagctgctg gaggtcctca gcacacccac gcccttcatc attggggtca acgcggcctt
1621 ccaggcagag acccaggagc tgctcgatgt gattgttgct gatctggatg gagggacggt
1681 caccattcct gagtgtgtgc acattccacc cttgccagag ccactgcaga gtcagacgca
1741 cagtgtgctg agcatggtcc tggacccgga gctggagttg gctgacctcg ccttccctcc
1801 gcccacgaca tccacctcct ccctgaagat gcaggacaag gagctgcgcg cggtcttcct
1861 gcggctgttc gctcagctgc tgcagggcta tcgctggtgc ctgcacgtcg tgcgcatcca
1921 cccggagcct gtcatccgct tccataaggc agccttcctg ggccagcgtg ggctggtaga
1981 ggacgatttc ctgatgaagg tgctggaggg catggccttt gctggctttg tgtcagagcg
2041 tggggtccca taccgcccta cggacctgtt cgatgagctg gtggcccacg aggtggcaag
2101 gatgcgggcg gatgagaacc acccccagcg tgtcctgcgt cacgtccagg aactggcaga
2161 gcagctctac aagaacgaga acccgtaccc agccgtggcg atgcacaagg tacagaggcc
2221 cggtgagagc agccacctgc gacgggtgcc ccgacccttc ccccggctgg atgagggcac
2281 cgtgcagtgg atcgtggacc aggctgcagc caagatgcag ggtgcacccc cagctgtgaa
2341 ggccgagagg aggaccaccg tgccctcagg gccccccatg actgccatac tggagcggtg
2401 cagtgggctg catgtcaaca gcgcccggcg gctggaggtt gtgcgcaact gcatctccta
2461 cgtgtttgag gggaaaatgc ttgaggccaa gaagctgctc ccagccgtgt tgagggccct
2521 gaaggggcga gctgcccgcc gctgcctcgc ccaggagctg cacctgcatg tgcagcagaa
2581 ccgtgcggtc ctggaccacc agcagtttga ctttgtcgtc cgtatgatga actgctgcct
2641 gcaggactgc acttctctgg acgagcatgg cattgcggcg gctctgctgc ctctggtcac
2701 agccttctgc cggaagctga gcccgggggt gacgcagttt gcatacagct gtgtgcagga
2761 gcacgtggtg tggagcacgc cacagttctg ggaggccatg ttctatgggg atgtgcagac
2821 tcacatccgg gccctctacc tggagcccac ggaggacctg gcccccgccc aggaggttgg
2881 ggaggcacct tcccaggagg acgagcgctc tgccctagac gtggcttctg agcagcggcg
2941 cttgtggcca actctgagtc gtgagaagca gcaggagctg gtgcagaagg aggagagcac
3001 ggtgttcagc caggccatcc actatgccaa ccgcatgagc tacctcctcc tgcccctgga
3061 cagcagcaag agccgcctac ttcgggagcg tgccgggctg ggcgacctgg agagcgccag
3121 caacagcctg gtcaccaaca gcatggctgg cagtgtggcc gagagctatg acacggagag
3181 cggcttcgag gatgcagaga cctgcgacgt agctggggct gtggtccgct tcatcaaccg
3241 ctttgtggac aaggtctgca cggagagtgg ggtcaccagc gaccacctca aggggctgca
3301 tgtcatggtg ccagacattg tccagatgca catcgagacc ctggaggccg tgcagcggga
3361 gagccggagg ctgccgccca tccagaagcc caagctgctg cggccgcgcc tgctgccggg
3421 tgaggagtgt gtgctggacg gcctgcgcgt ctacctgctg ccggatgggc gtgaggaggg
3481 cgcggggggc agtgctgggg gaccagcatt gctcccagct gagggcgccg tcttcctcac
3541 cacgtaccgg gtcatcttca cggggatgcc cacggacccc ctggttgggg agcaggtggt
3601 ggtccgctcc ttcccggtgg ctgcgctgac caaggagaag cgcatcagcg tccagacccc
3661 tgtggaccag ctcctgcagg acgggctcca gctgcgctcc tgcacattcc agctgctgaa
3721 aatggccttt gacgaggagg tggggtctga cagcgccgag ctcttccgta agcagctgca
3781 taagctgcgg tacccgccgg acatcagggc cacctttgcg ttcaccttgg gctctgccca
3841 cacacctggc cggccaccgc gagtcaccaa ggacaagggt ccttccctca gaaccctgtc
3901 ccggaacctg gtcaagaacg ccaagaagac catcgggcgg cagcatgtca ctcgcaagaa
3961 gtacaacccc cccagctggg agcaccgggg ccagccgccc cctgaggacc aggaggacga
4021 gatctcagtg tcggaggagc tggagcccag cacgctgacc ccgtcctcag ccctgaagcc
4081 ctccgaccgc atgaccatga gcagcctggt ggaaaggtaa gtatcaaggt tacaagacag
4141 gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt
4201 atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
4261 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
4321 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
4381 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
4441 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4501 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
4561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
4681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4741 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
4801 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
4861 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
4921 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4981 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
5041 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
5101 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
5161 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
5221 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
5281 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
5341 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
5401 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5461 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5521 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5581 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5641 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5701 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5761 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5821 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5881 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5941 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
6001 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
6061 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
6121 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
6181 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
6241 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
6301 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
6361 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6421 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
6481 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6541 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6601 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6661 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
6721 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6781 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6841 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6901 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6961 ccttttgctc acatgt
//
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