| 质粒名称: | pSprayer |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165148 |
| 存储实验室: | Paul Khavari |
|
载体骨架基本信息 |
|
| 载体名称: | pUC-like |
| 载体出品公司: | Clontech |
| 空载体大小: | 4531bp |
| 完整质粒大小: | 4531bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | BASU-GAL4 |
|
文献引用方法 |
|
| 材料和方法部分: | pSprayer was a gift from Paul Khavari (Addgene plasmid # 165148 ; http://n2t.net/addgene:165148 ; RRID:Addgene_165148) |
| 参考文献部分: | PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V, Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y, Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA. Nat Methods. 2022 Aug;19(8):959-968. doi: 10.1038/s41592-022-01552-w. Epub 2022 Aug 4. 10.1038/s41592-022-01552-w PubMed 35927480 |
买家导航
pSprayer载体质粒基本信息
pSprayer质粒图谱载体图谱和pSprayer载体序列质粒序列多克隆位点信息
pSprayer质粒载体简介
pSprayer质粒序列载体序列
LOCUS pSprayer 4531 bp DNA circular SYN 14-APR-2025
DEFINITION Sprayer plasmid for PROBER encoding BASU-GAL4 fusion.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4531)
AUTHORS Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V,
Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y,
Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA
TITLE PROBER identifies proteins associated with programmable
sequence-specific DNA in living cells.
JOURNAL Nat Methods. 2022 Aug;19(8):959-968. doi:
10.1038/s41592-022-01552-w. Epub 2022 Aug 4.
PUBMED 35927480
REFERENCE 2 (bases 1 to 4531)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41592-022-01552-w"; journalName: "Nat Methods"; date:
"2022-08"; volume: "19"; issue: "8"; pages: "959-968"
COMMENT Sequence Label: pSprayer
FEATURES Location/Qualifiers
source 1..4531
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 540..551
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 594..614
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 621..647
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1929..1955
/codon_start=1
/product="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
/label=c-myc NLS
/translation="PAAKRVKLD"
primer_bind complement(1990..2007)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1996..2107
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2266..2401
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2328..2347
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2459..2476)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2630..3218)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2710..2729)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(3389..4180,4181..4249))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4012..4031
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4250..4354)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccgaa
541 ttgaaggaag gccgtcaagg ccgcatttgg cataacgact catatttatg gctcctaaaa
601 agaagcgtaa agtcaagcta tacccatacg atgtgcctga ctatgctggc aagttgtctg
661 aatcagagat taggtttgga ctgaaaacgg aggtgatggg ccaacatctt atctatcacg
721 atgttttgtc cagcacacaa aagacagccc atgaattggc aaataacaat gcccccgagg
781 gtaccctcgt ggttgcagat aaacagacag ccggtagggg cgggatgagc cgagtgtggc
841 attctcaaga aggaaacgga gtatggatga gtttgatatt gcgcccggac attccgctcc
901 aaaagacgcc ccaacttacc ttgctggcag ctgtagcggt ggttcaggga atagaagagg
961 ccgcgggcat acagactgat ataaagtggc cgaacgacat actcattaac gggaaaaaaa
1021 cagtagggat acttaccgaa atgcaagctg aggaggatcg agtgcgctcc gtgattattg
1081 gtattggcat taatgtaaat cagcaaccca atgatttccc cgatgagctc aaggacattg
1141 cgacgagtct gtcccaggca gctggggaaa agatcgacag ggcaggtgta atccagcata
1201 tcttgctttg tttcgaaaag cgataccgag actacatgac ccacggtttc acgcccatca
1261 agctcttgtg ggagtcatac gccctcggta ttggcacgaa catgagggcg agaactctca
1321 acgggacatt ttacggtaag gcgttgggta ttgacgacga aggggtactt ttgttggaaa
1381 ctaatgaagg cattaagaag atatacagcg cggacatcga acttggctgc cccgggggat
1441 ccggaggagg tggaagcgga ggaggaggaa gcggaggagg aggtagcctc gaggtatact
1501 cttctatcga acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa
1561 aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca
1621 aaaggtctcc gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg
1681 aacagctatt tctactgatt tttccgcgag aagaccttga catgattttg aaaatggatt
1741 ctttacagga tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag
1801 atgccgtcac agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc
1861 atagaataag tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga
1921 ctgtatcgcc cgcagctaag cgagtcaaat tggattgaga attccctgct ctcaaaactg
1981 tcttctcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
2041 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
2101 ttgcatcgaa gacagtcagc cttgtggaat gtgtgtcagt tagagtagtt catgtcatct
2161 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
2221 ttgtttaaac cgtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
2281 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
2341 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
2401 ctaggctttt gcaaaaagct cggagaggcg gtttgcgtat tgggcgctct tccgcttcct
2461 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
2521 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
2581 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
2641 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2701 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2761 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
2821 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
2881 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
2941 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
3001 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
3061 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
3121 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
3181 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
3241 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
3301 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
3361 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
3421 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
3481 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
3541 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
3601 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
3661 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
3721 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
3781 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
3841 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
3901 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
3961 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
4021 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
4081 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
4141 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
4201 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
4261 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
4321 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
4381 acgtcgacgg atcgggagat ctctagagat ccgactcgag tactaggatc cattaggcgg
4441 ccgcgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
4501 cattagttca tagcccatat atggagttcc g
//
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