psiCHECK-2-h2.525894

LOCUS      psiCHECK-2-h2.525894          6610 bp DNA     circular SYN 03-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2009
                     /label=-9
     polyA_signal    2025..2073
                     /note="synthetic polyadenylation signal"
     promoter        2082..2833
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2863..2872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2869..4521
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3828..3836
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4565..4686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4819..4923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4924..5784
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5955..6543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 cttggattcc attattttgc caaatcattt atttataagg taaattccta gaagtaacat
     1741 ttctgggtca aacgacgtta gtaatattga taggtacaaa ggctttttgt tattgtttgt
     1801 tttcattttt attaaagtta tgcatacaag cacatagatt taagaaacaa ttctacaatt
     1861 tttttaaaag gataagaaaa gcagacactc ccctcacagt gcttgcaggc acctctttta
     1921 gctttttttt tattttcaat ttaccctctt gtctctagat gatacgctgc ttggtgaatt
     1981 ttgtttcagc gttacccact gactccccgg cggccgctgg ccgcaataaa atatctttat
     2041 tttcattaca tctgtgtgtt ggttttttgt gtgaggatct aaatgagtct tcggacctcg
     2101 cgggggccgc ttaagcggtg gttagggttt gtctgacgcg gggggagggg gaaggaacga
     2161 aacactctca ttcggaggcg gctcggggtt tggtcttggt ggccacgggc acgcagaaga
     2221 gcgccgcgat cctcttaagc acccccccgc cctccgtgga ggcgggggtt tggtcggcgg
     2281 gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc cccagagtgt gaccttttcg
     2341 gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg acgggctcgc tgggtcctag
     2401 gctccatggg gaccgtatac gtggacaggc tctggagcat ccgcacgact gcggtgatat
     2461 taccggagac cttctgcggg acgagccggg tcacgcggct gacgcggagc gtccgttggg
     2521 cgacaaacac caggacgggg cacaggtaca ctatcttgtc acccggaggc gcgagggact
     2581 gcaggagctt cagggagtgg cgcagctgct tcatccccgt ggcccgttgc tcgcgtttgc
     2641 tggcggtgtc cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc
     2701 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     2761 ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     2821 gcgacccgct taaaagcttg gcattccggt actgttggta aagccaccat ggccgatgct
     2881 aagaacatta agaagggccc tgctcccttc taccctctgg aggatggcac cgctggcgag
     2941 cagctgcaca aggccatgaa gaggtatgcc ctggtgcctg gcaccattgc cttcaccgat
     3001 gcccacattg aggtggacat cacctatgcc gagtacttcg agatgtctgt gcgcctggcc
     3061 gaggccatga agaggtacgg cctgaacacc aaccaccgca tcgtggtgtg ctctgagaac
     3121 tctctgcagt tcttcatgcc agtgctgggc gccctgttca tcggagtggc cgtggcccct
     3181 gctaacgaca tttacaacga gcgcgagctg ctgaacagca tgggcatttc tcagcctacc
     3241 gtggtgttcg tgtctaagaa gggcctgcag aagatcctga acgtgcagaa gaagctgcct
     3301 atcatccaga agatcatcat catggactct aagaccgact accagggctt ccagagcatg
     3361 tacacattcg tgacatctca tctgcctcct ggcttcaacg agtacgactt cgtgccagag
     3421 tctttcgaca gggacaaaac cattgccctg atcatgaaca gctctgggtc taccggcctg
     3481 cctaagggcg tggccctgcc tcatcgcacc gcctgtgtgc gcttctctca cgcccgcgac
     3541 cctattttcg gcaaccagat catccccgac accgctattc tgagcgtggt gccattccac
     3601 cacggcttcg gcatgttcac caccctgggc tacctgattt gcggctttcg ggtggtgctg
     3661 atgtaccgct tcgaggagga gctgttcctg cgcagcctgc aagactacaa aattcagtct
     3721 gccctgctgg tgccaaccct gttcagcttc ttcgctaaga gcaccctgat cgacaagtac
     3781 gacctgtcta acctgcacga gattgcctct ggcggcgccc cactgtctaa ggaggtgggc
     3841 gaagccgtgg ccaagcgctt tcatctgcca ggcatccgcc agggctacgg cctgaccgag
     3901 acaaccagcg ccattctgat taccccagag ggcgacgaca agcctggcgc cgtgggcaag
     3961 gtggtgccat tcttcgaggc caaggtggtg gacctggaca ccggcaagac cctgggagtg
     4021 aaccagcgcg gcgagctgtg tgtgcgcggc cctatgatta tgtccggcta cgtgaataac
     4081 cctgaggcca caaacgccct gatcgacaag gacggctggc tgcactctgg cgacattgcc
     4141 tactgggacg aggacgagca cttcttcatc gtggaccgcc tgaagtctct gatcaagtac
     4201 aagggctacc aggtggcccc agccgagctg gagtctatcc tgctgcagca ccctaacatt
     4261 ttcgacgccg gagtggccgg cctgcccgac gacgatgccg gcgagctgcc tgccgccgtc
     4321 gtcgtgctgg aacacggcaa gaccatgacc gagaaggaga tcgtggacta tgtggccagc
     4381 caggtgacaa ccgccaagaa gctgcgcggc ggagtggtgt tcgtggacga ggtgcccaag
     4441 ggcctgaccg gcaagctgga cgcccgcaag atccgcgaga tcctgatcaa ggctaagaaa
     4501 ggcggcaaga tcgccgtgta ataattctag agtcggggcg gccggccgct tcgagcagac
     4561 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4621 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4681 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     4741 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccaggtggc
     4801 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4861 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4921 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4981 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5041 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5101 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5161 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5221 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5281 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5341 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5401 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5461 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5521 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5581 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5641 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5701 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5761 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5821 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6601 atggctcgac
//