| 质粒名称: | pAAV.CMV.LacZ.bGH |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 105531 |
| 存储实验室: | James M. Wilson |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LacZ |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV.CMV.LacZ.bGH was a gift from James M. Wilson (Addgene plasmid # 105531 ; http://n2t.net/addgene:105531 ; RRID:Addgene_105531) |
买家导航
pAAV.CMV.LacZ.bGH载体质粒基本信息
pAAV.CMV.LacZ.bGH质粒图谱载体图谱和pAAV.CMV.LacZ.bGH载体序列质粒序列多克隆位点信息
pAAV.CMV.LacZ.bGH质粒载体简介
pAAV.CMV.LacZ.bGH质粒序列载体序列
LOCUS pAAV.CMV.LacZ.bGH 7152 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION AAV expression of LacZ from CMV promoter.
ACCESSION .
VERSION .
KEYWORDS pAAV.CMV.LacZ.bGH
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7152)
TITLE AAV plasmids
REFERENCE 2 (bases 1 to 7152)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7152
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 31..61
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 69..85
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 74..96
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 93..109
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 93..109
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
repeat_region 147..276
/label=AAV2 ITR
enhancer 417..720
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 721..924
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 875..895
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 965..4039
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/label=lacZ
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
primer_bind complement(985..1001)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4047..4064)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4053..4260
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4348..4477
/label=AAV2 ITR
primer_bind complement(4496..4512)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4654..5109
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4741..4760)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4951..4972
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 5135..5239
/gene="bla"
/label=AmpR promoter
CDS 5240..6100
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5458..5477)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6271..6859
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6760..6779
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7013..7030
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7147..16
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
61 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
121 cagatttaat taaggcctta attaggctgc gcgctcgctc gctcactgag gccgcccggg
181 caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca
241 gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat
301 gctacttatc tacgtagcca tgctctagga agatcggaat tcgcccttaa gctagctagt
361 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
421 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
481 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
541 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
601 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
661 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
721 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
781 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
841 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
901 tgggaggtct atataagcag agctggttta gtgaaccgtc agatcctgca gataagcggc
961 cgccatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg actgggaaaa
1021 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
1081 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
1141 gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct
1201 tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc
1261 catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa
1321 tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca
1381 gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg
1441 ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg
1501 cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga
1561 agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc
1621 gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc
1681 tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt
1741 ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat
1801 tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc
1861 gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc
1921 cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga
1981 aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga
2041 gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct
2101 gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg
2161 gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca
2221 cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga
2281 acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct
2341 ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt
2401 cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat
2461 tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg
2521 gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata
2581 cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca
2641 gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata
2701 tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga
2761 tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac
2821 ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt
2881 gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct
2941 ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca
3001 gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt
3061 acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca
3121 gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc
3181 gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt
3241 taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac
3301 gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc
3361 gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc
3421 cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac
3481 cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat
3541 tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca
3601 tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct
3661 cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg
3721 ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg
3781 ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa
3841 catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc
3901 ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc
3961 ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt
4021 ggtctggtgt caaaaataag gatctgcctc gactgtgcct tctagttgcc agccatctgt
4081 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
4141 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
4201 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
4261 ctcgagttaa gggcgaattc ccgataagga tcttcctaga gcatggctac gtagataagt
4321 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
4381 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
4441 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct aattcactgg
4501 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
4561 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
4621 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
4681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
4741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
4801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
4861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
4921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
4981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
5041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
5101 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
5161 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
5221 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
5281 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
5341 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
5401 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
5461 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
5521 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
5581 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
5641 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
5701 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
5761 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
5821 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
5881 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
5941 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
6001 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
6061 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
6121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
6181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
6241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
6301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
6361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
6421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
6481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
6541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
6601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
6661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
6721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
6781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
6841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
6901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
6961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
7021 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
7081 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
7141 cgcaattaat gt
//
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