| 质粒名称: | pAAV-CAG-mScarlet-H-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000715 |
| 产品用途: | AAV, experess mScarlet-H and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mScarlet-H, EGFP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
买家导航
pAAV-CAG-mScarlet-H-P2A-EGFP载体质粒基本信息
pAAV-CAG-mScarlet-H-P2A-EGFP质粒图谱载体图谱和pAAV-CAG-mScarlet-H-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-mScarlet-H-P2A-EGFP质粒载体简介
pAAV-CAG-mScarlet-H-P2A-EGFP质粒序列载体序列
LOCUS pAAV-CAG-mScarlet-H-P2A-EGFP 6229 bp DNA circular SYN 28-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6229)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6229
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 957..1006
/label=H-F1
regulatory 977..982
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1678
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-H
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
HALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
primer_bind complement(1452..1474)
/label=mScarlet-H-R
primer_bind 1455..1494
/label=mScarlet-H-F
primer_bind complement(1653..1702)
/label=H-R1
CDS 1703..1759
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1772..2488
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 2513..3101
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2984..2995)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3144..3265
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3793..4381)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4552..5412)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5413..5517)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5543..5998)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtgag caagggcgag gcagtgatca aggagttcat
1021 gcggttcaag gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga
1081 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
1141 ccccctgccc ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt
1201 caccaagcac cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa
1261 gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc
1321 cctggaggac ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga
1381 cggccccgta atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc
1441 cgaggacggc gtgctgaagg gcgacattaa gcacgccctg cgcctgaagg acggcggccg
1501 ctacctggcg gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc
1561 ctacaacgtc gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga
1621 acagtacgaa cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagcg
1681 accggttcac gtgggaagtg gggctacaaa cttctcactg ctgaaacagg ccggggatgt
1741 ggaggaaaac ccaggaccac acgtgactag tgtgagcaag ggcgaggagc tgttcaccgg
1801 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
1861 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
1921 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
1981 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
2041 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
2101 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
2161 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
2221 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
2281 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
2341 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
2401 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
2461 tctcggcatg gacgagctgt acaagtaaga attcgatatc aagcttatcg ataatcaacc
2521 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
2581 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
2641 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
2701 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
2761 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
2821 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
2881 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
2941 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
3001 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
3061 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacccgg
3121 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
3181 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
3241 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
3301 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
3361 cgataaggat cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta
3421 actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
3481 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
3541 gcgagcgagc gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
3601 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
3661 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
3721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
3781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
3841 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
3901 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
3961 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
4021 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
4081 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
4141 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
4201 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
4261 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
4321 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
4381 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
4441 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
4501 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
4561 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4621 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
4681 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
4741 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
4801 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
4861 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
4921 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
4981 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
5041 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
5101 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
5161 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
5221 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
5281 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
5341 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
5401 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
5461 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5521 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
5581 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
5641 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
5701 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
5761 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
5821 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
5881 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
5941 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
6001 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
6061 ttacgccagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg
6121 gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac
6181 tccatcacta ggggttcctt gtagttaatg attaacccgc catgctact
//
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