| 质粒名称: | MSCV JMJD3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21212 |
| 存储实验室: | Paul Khavari |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCVpuro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6300 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | JMJD3 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
Flag（插入N端子）
HA（插入件上的N端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pLXSN-5 |
| 3' 测序引物 | MSCV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | MSCV JMJD3 was a gift from Paul Khavari (Addgene plasmid # 21212 ; http://n2t.net/addgene:21212 ; RRID:Addgene_21212) |
| 参考文献部分: | Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA. Genes Dev. 2008 Jul 15. 22(14):1865-70. 10.1101/gad.1673508 PubMed 18628393 |
买家导航
MSCV JMJD3载体 MSCVJMJD3质粒基本信息
MSCV JMJD3质粒图谱 MSCVJMJD3载体图谱和MSCV JMJD3载体序列 MSCVJMJD3质粒序列多克隆位点信息
MSCV JMJD3质粒 MSCVJMJD3载体简介
MSCV JMJD3质粒序列 MSCVJMJD3载体序列
LOCUS MSCV JMJD3 11521 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS MSCV JMJD3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11521)
AUTHORS Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA
TITLE Control of differentiation in a self-renewing mammalian tissue by
the histone demethylase JMJD3.
JOURNAL Genes Dev. 2008 Jul 15. 22(14):1865-70.
PUBMED 18628393
REFERENCE 2 (bases 1 to 11521)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11521
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(63..80)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(234..822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(314..333)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(993..1853)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1616..1635
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1854..1958)
/gene="bla"
/label=AmpR promoter
primer_bind 2026..2044
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2082..2104)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2204..2223
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2417..2439
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2567..2586
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 2786..3302
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 3366..3707
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 3774..4190
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 4120..4142
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 4158..4174
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 4203..4212
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4212..4235
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 4248..4274
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind 4290..4314
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(4735..4746)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 5095..5118
/codon_start=1
/product="8xHis affinity tag"
/label=8xHis
/translation="HHHHHHHH"
CDS 5098..5121
/codon_start=1
/product="8xHis affinity tag"
/label=8xHis
/translation="HHHHHHHH"
CDS 6585..6614
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 6588..6617
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
protein_bind complement(9408..9432)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 9462..10014
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(9606..9623)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 9833..9852
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 10026..10625
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(10026..10045)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 10522..10542
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 10701..11215
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 11377..11393
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11401..11431)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11446..11467
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
1981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
2041 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
2101 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
2161 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
2221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
2281 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
2341 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
2401 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
2461 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
2521 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
2581 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
2641 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
2701 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
2761 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
2821 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
2881 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
2941 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
3001 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
3061 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
3121 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
3181 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
3241 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
3301 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
3361 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
3421 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
3481 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
3541 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
3601 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
3661 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
3721 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
3781 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
3841 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
3901 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
3961 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
4021 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
4081 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
4141 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
4201 ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg
4261 ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagca ggctccgcgg
4321 ccgccccctt caccggatcc atgcatcggg cagtggatcc tccaggggcc cgcgctgcac
4381 gggaagcctt tgcccttggg ggcctgagct gtgctggggc ctggagctcc tgcccgcctc
4441 atccccctcc tcgtagcgca tggctgcctg gaggcagatg ctcagccagc attgggcagc
4501 ccccgcttcc tgctccccta cccccttcac atggcagtag ttctgggcac cccagcaaac
4561 catattatgc tccaggggcg cccactccaa gacccctcca tgggaagctg gaatccctgc
4621 atggctgtgt gcaggcattg ctccgggagc cagcccagcc agggctttgg gaacagcttg
4681 ggcaactgta cgagtcagag cacgatagtg aggaggccac acgctgctac cacagcgccc
4741 ttcgatacgg aggaagcttc gctgagctgg ggccccgcat tggccgactg cagcaggccc
4801 agctctggaa ctttcatact ggctcctgcc agcaccgagc caaggtcctg cccccactgg
4861 agcaagtgtg gaacttgcta caccttgagc acaaacggaa ctatggagcc aagcggggag
4921 gtcccccggt gaagcgagct gctgaacccc cagtggtgca gcctgtgcct cctgcagcac
4981 tctcaggccc ctcaggggag gagggcctca gccctggagg caagcgaagg agaggctgca
5041 actctgaaca gactggcctt cccccagggc tgccactgcc tccaccacca ttaccaccac
5101 caccaccacc accaccacca cccctgcctg gcctggctac cagcccccca tttcagctaa
5161 ccaagccagg gctgtggagt accctgcatg gagatgcctg gggcccagag cgcaagggtt
5221 cagcaccccc agagcgccag gagcagcggc actcgctgcc tcacccatat ccatacccag
5281 ctccagcgta caccgcgcac ccccctggcc accggctggt cccggctgct cccccaggcc
5341 caggcccccg ccccccagga gcagagagcc atggctgcct gcctgccacc cgtccccccg
5401 gaagtgacct tagagagagc agagttcaga ggtcgcggat ggactccagc gtttcaccag
5461 cagcaaccac cgcctgcgtg ccttacgccc cttcccggcc ccctggcctc cccggcacca
5521 ccaccagcag cagcagtagc agcagcagca acactggtct ccggggcgtg gagccgaacc
5581 caggcattcc cggcgctgac cattaccaaa ctcccgcgct ggaggtctct caccatggcc
5641 gcctggggcc ctcggcacac agcagtcgga aaccgttctt gggggctccc gctgccactc
5701 cccacctatc cctgccacct ggaccttcct cacccccccc cccccccctg tccccgcctc
5761 ttacgccccc caccaccccc tgcctggttg aagggtccgg cctgccgggc agcccgagag
5821 gatggagaga tcttagaaga gctcttcttt gggactgagg gacccccccg ccctgcccca
5881 ccacccctcc cccatcgcga gggcttcttg gggcctccgg cctcccgctt ttctgtgggc
5941 actcaggatt ctcacacccc tcccactccc ccaaccccaa ccaccagcag tagcaacagc
6001 aacagtggca gccacagcag cagccctgct gggcctgtgt cctttccccc accaccctat
6061 ctggccagaa gtatagaccc ccttccccgg cctcccagcc cagcacagaa cccccaggac
6121 ccacctcttg tacccctgac tcttgccctg cctccagccc ctccttcctc ctgccaccaa
6181 aatacctcag gaagcttcag gcgcccggag agcccccggc ccagggtctc cttcccaaag
6241 acccccgagg tggggccggg gccaccccca ggccccctga gtaaagcccc ccagcctgtg
6301 ccgcccgggg ttggggagct gcctgcccga ggccctcgac tctttgattt tccccccact
6361 ccgctggagg accagtttga ggagccagcc gaattcaaga tcctacctga tgggctggcc
6421 aacatcatga agatgctgga cgaatccatt cgcaaggaag aggaacagca acaacacgaa
6481 gcaggcgtgg ccccccaacc cccgctgaag gagccctttg catctctgca gtctcctttc
6541 cccaccgaca cagcccccac cactactgct cctgctgtcg ccgtcaccac caccaccacc
6601 accaccacca ccaccacggc cacccaggaa gaggagaaga agccaccacc agccctacca
6661 ccaccaccgc ctctagccaa gttccctcca ccctctcagc cacagccacc accaccccca
6721 ccccccagcc cggccagcct gctcaaatcc ttggcctccg tgctggaggg acaaaagtac
6781 tgttatcggg ggactggagc agctgtttcc acccggcctg ggcccttgcc caccactcag
6841 tattcccctg gccccccatc aggtgctacc gccctgccgc ccacctcagc ggcccctagc
6901 gcccagggct ccccacagcc ctctgcttcc tcgtcatctc agttctctac ctcaggcggg
6961 ccctgggccc gggagcgcag ggcgggcgaa gagccagtcc cgggccccat gacccccacc
7021 caaccgcccc cacccctatc tctgccccct gctcgctctg agtctgaggt gctagaagag
7081 atcagccggg cttgcgagac ccttgtggag cgggtgggcc ggagtgccac tgacccagcc
7141 gacccagtgg acacagcaga gccagcggac agtgggactg agcgactgct gccccccgca
7201 caggccaagg aggaggctgg cggggtggcg gcagtgtcag gcagctgtaa gcggcgacag
7261 aaggagcatc agaaggagca tcggcggcac aggcgggcct gtaaggacag tgtgggtcgt
7321 cggccccgtg agggcagggc aaaggccaag gccaaggtcc ccaaagaaaa gagccgccgg
7381 gtgctgggga acctggacct gcagagcgag gagatccagg gtcgtgagaa gtcccggccc
7441 gatcttggcg gggcctccaa ggccaagcca cccacagctc cagcccctcc atcagctcct
7501 gcaccttctg cccagcccac acccccgtca gcctctgtcc ctggaaagaa ggctcgggag
7561 gaagccccag ggccaccggg tgtcagccgg gccgacatgc tgaagctgcg ctcacttagt
7621 gaggggcccc ccaaggagct gaagatccgg ctcatcaagg tagagagtgg tgacaaggag
7681 acctttatcg cctctgaggt ggaagagcgg cggctgcgca tggcagacct caccatcagc
7741 cactgtgctg ctgacgtcgt gcgcgccagc aggaatgcca aggtgaaagg gaagtttcga
7801 gagtcctacc tttcccctgc ccagtctgtg aaaccgaaga tcaacactga ggagaagctg
7861 ccccgggaaa aactcaaccc ccctacaccc agcatctatc tggagagcaa acgggatgcc
7921 ttctcacctg tcctgctgca gttctgtaca gaccctcgaa atcccatcac agtgatccgg
7981 ggcctggcgg gctccctgcg gctcaacttg ggcctcttct ccaccaagac cctggtggaa
8041 gcgagtggcg aacacaccgt ggaagttcgc acccaggtgc agcagccctc agatgagaac
8101 tgggatctga caggcactcg gcagatctgg ccttgtgaga gctcccgttc ccacaccacc
8161 attgccaagt acgcacagta ccaggcctca tccttccagg agtctctgca ggaggagaag
8221 gagagtgagg atgaggagtc agaggagcca gacagcacca ctggaacccc tcctagcagc
8281 gcaccagacc cgaagaacca tcacatcatc aagtttggca ccaacatcga cttgtctgat
8341 gctaagcggt ggaagcccca gctgcaggag ctgctaaagc tgcccgcctt catgcgggta
8401 acatccacgg gcaacatgct gagccacgtg ggccacacca tcctgggcat gaacacggtg
8461 cagctgtaca tgaaggtgcc cggcagccga acgccaggcc accaggagaa taacaacttc
8521 tgctccgtca acatcaacat tggcccaggc gactgcgagt ggttcgcggt gcacgagcac
8581 tactgggaga ccatcagcgc tttctgtgat cggcacggcg tggactactt gacgggttcc
8641 tggtggccaa tcctggatga tctctatgca tccaatattc ctgtgtaccg cttcgtgcag
8701 cgacccggag acctcgtgtg gattaatgcg gggactgtgc actgggtgca ggccaccggc
8761 tggtgcaaca acattgcctg gaacgtgggg cccctcaccg cctatcagta ccagctggcc
8821 ctggaacgat acgagtggaa tgaggtgaag aacgtcaaat ccatcgtgcc catgattcac
8881 gtgtcatgga acgtggctcg cacggtcaaa atcagcgacc ccgacttgtt caagatgatc
8941 aagttctgcc tgctgcagtc catgaagcac tgccaggtgc aacgcgagag cctggtgcgg
9001 gcagggaaga aaatcgctta ccagggccgt gtcaaggacg agccagccta ctactgcaac
9061 gagtgcgatg tggaggtgtt taacatcctg ttcgtgacaa gtgagaatgg cagccgcaac
9121 acgtacctgg tacactgcga gggctgtgcc cggcgccgca gcgcaggcct gcagggcgtg
9181 gtggtgctgg agcagtaccg cactgaggag ctggctcagg cctacgacgc cttcacgctg
9241 gtgagggccc ggcgggcgcg cgggcagcgg aggagggcac tggggcaggc tgcagggacg
9301 ggcttcggga gcccggccgc gcctttccct gagcccccgc cggctttctc cccccaggcc
9361 ccagccagca cgtcgcgatg actcgaggcg aaagggtggg cgcgccgacc cagctttctt
9421 gtacaaagtg gttcgataac gaattccgcc ccccccccct aacgttactg gccgaagccg
9481 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
9541 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
9601 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
9661 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
9721 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
9781 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
9841 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
9901 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
9961 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
10021 caaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
10081 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
10141 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
10201 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
10261 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
10321 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
10381 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
10441 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
10501 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
10561 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
10621 cctgatgcat cgatgtcgac ctgcagccaa gcttatcgat aaaataaaag attttattta
10681 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
10741 taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag
10801 gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
10861 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
10921 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
10981 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
11041 caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc
11101 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
11161 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta
11221 acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc
11281 tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa tagttcatta
11341 tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca caattccaca
11401 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
11461 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
11521 g
//
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