pAAVLINKv2-SCP1-Cre-UDeg3a-DSP-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-DSP-C-FLAG            7052 bp DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3765
                     /codon_start=1
                     /label=DSP
                     /translation="GV**TL*TVSFCQKMSPWPGG*LIEICIDP*MIPEIVRKTLWIQS
                     PKRRSVTCS*RNGAESNHILVCSCFQYRREACPSKESDNL*PSLS**ILVY*DRPLSMN
                     WNLVRFLMTRLVRELRTSSRVQAA*QAYTMRPQNRSLAFMRP*KLA*SDLVLLWSCWKP
                     KQLLAL*WILLAT*GYQWRKPTREVWWALSSKRSSCLQNELSLGIMILKQETSSLCSKP
                     *IRNSSKRATVFAY*KHRSQLGGSLTQRRAIVYQLT*HIRGAISMRNSVRFSQIQVMIP
                     KDFLTPTLKKILPICN*KKDALRMRKQGSVFCL*KKRRNRCRHHKRIPSGSVEWS*LTQ
                     KPIKKCLFRRPTRRA*LIMKPSKNCVSRNVNGKK*PSRDQMAPPGWSW*IERQAVSMIF
                     KMLLTRALLTGSSLISTDPAASASLNLLT*SP*KMVSAPAAAWAVVSAMMFLAAPDMNQ
                     *VRFPPYPASGI*P*GAALFQTPWKNRAPLQPSLTQKTWRKSPLQKV*SGASLTASRVR
                     GFWRLRPAQVASSTQPRARSCHFRTQSPRV*LTKTWPPG*SLLRKPS*ASRV*RERRRC
                     QQQRQ*KKNGSRMRLASASWSSSTSREVLLTRKCMGG*APKKPSGRGS*MAAPHRGCKT
                     PAAMPKS*PAPKPN*KYPIRMP*IAPW*KISLGCAFWKPPPCRPRAYPALTTCLRLRAP
                     APAPARDLAPDLAPGPAVGPGEEALTPQGILPTLIPTHLAVVLLG"
     misc_feature    2591..2596
                     /label=SDS
     CDS             3772..3837
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3841..4272
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3985..3990
                     /label=SDS
     repeat_region   4315..4455
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4315..4444
                     /label=AAV2 ITR
     rep_origin      4530..4985
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5267..5371
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5372..6232
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6403..6991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggg tgtagtagac
     1561 cctgtgaaca gtgtcttttt gccaaaagat gtcgccttgg cccgggggct gattgataga
     1621 gatttgtatc gatccctgaa tgatccccga gatagtcaga aaaactttgt ggatccagtc
     1681 accaaaaaga aggtcagtta cgtgcagctg aaggaacggt gcagaatcga accacatact
     1741 ggtctgctct tgctttcagt acagaagaga agcatgtcct tccaaggaat cagacaacct
     1801 gtgaccgtca ctgagctagt agattctggt atattgagac cgtccactgt caatgaactg
     1861 gaatctggtc agatttctta tgacgaggtt ggtgagagaa ttaaggactt cctccagggt
     1921 tcaagctgca tagcaggcat atacaatgag accacaaaac agaagcttgg catttatgag
     1981 gccatgaaaa ttggcttagt ccgacctggt actgctctgg agttgctgga agcccaagca
     2041 gctactggct ttatagtgga tcctgttagc aacttgaggt taccagtgga ggaagcctac
     2101 aagagaggtc tggtgggcat tgagttcaaa gagaagctcc tgtctgcaga acgagctgtc
     2161 actgggtata atgatcctga aacaggaaac atcatctctt tgttccaagc catgaataag
     2221 gaactcatcg aaaagggcca cggtattcgc ttattagaag cacagatcgc aactgggggg
     2281 atcattgacc caaaggagag ccatcgttta ccagttgaca tagcatataa gaggggctat
     2341 ttcaatgagg aactcagtga gattctctca gatccaagtg atgataccaa aggatttttt
     2401 gaccccaaca ctgaagaaaa tcttacctat ctgcaactaa aagaaagatg cattaaggat
     2461 gaggaaacag ggctctgtct tctgcctctg aaagaaaaga agaaacaggt gcagacatca
     2521 caaaagaata ccctcaggaa gcgtagagtg gtcatagttg acccagaaac caataaagaa
     2581 atgtctgttc aggaggccta caagaagggc ctaattgatt atgaaacctt caaagaactg
     2641 tgtgagcagg aatgtgaatg ggaagaaata accatcacgg gatcagatgg ctccaccagg
     2701 gtggtcctgg tagatagaaa gacaggcagt cagtatgata ttcaagatgc tattgacaag
     2761 ggccttgttg acaggaagtt ctttgatcag taccgatccg gcagcctcag cctcactcaa
     2821 tttgctgaca tgatctcctt gaaaaatggt gtcggcacca gcagcagcat gggcagtggt
     2881 gtcagcgatg atgtttttag cagctcccga catgaatcag taagtaagat ttccaccata
     2941 tccagcgtca ggaatttaac cataaggagc agctcttttt cagacaccct ggaagaatcg
     3001 agccccattg cagccatctt tgacacagaa aacctggaga aaatctccat tacagaaggt
     3061 atagagcggg gcatcgttga cagcatcacg ggtcagaggc ttctggaggc tcaggcctgc
     3121 acaggtggca tcatccaccc aaccacgggc cagaagctgt cacttcagga cgcagtctcc
     3181 cagggtgtga ttgaccaaga catggccacc aggctgaagc ctgctcagaa agccttcata
     3241 ggcttcgagg gtgtgaaggg aaagaagaag atgtcagcag cagaggcagt gaaagaaaaa
     3301 tggctcccgt atgaggctgg ccagcgcttc ctggagttcc agtacctcac gggaggtctt
     3361 gttgacccgg aagtgcatgg gaggataagc accgaagaag ccatccggaa ggggttcata
     3421 gatggccgcg ccgcacagag gctgcaagac accagcagct atgccaaaat cctgacctgc
     3481 cccaaaacca aattaaaaat atcctataag gatgccataa atcgctccat ggtagaagat
     3541 atcactgggc tgcgccttct ggaagccgcc tccgtgtcgt ccaagggctt acccagccct
     3601 tacaacatgt cttcggctcc gggctcccgc tccggctccc gctcgggatc tcgctccgga
     3661 tctcgctccg ggtcccgcag tgggtcccgg agaggaagct ttgacgccac agggaattct
     3721 tcctactctt attcctactc atttagcagt agttctattg ggcactgtac agactacaaa
     3781 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa
     3841 ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3901 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3961 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg
     4021 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4081 acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct
     4141 agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4201 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     4261 gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
     4321 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4381 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4441 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4501 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     4561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     4621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4741 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4861 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     4921 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4981 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5041 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5101 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5161 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5221 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5281 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5341 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5401 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5461 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5521 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5581 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5641 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5701 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5761 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5821 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5881 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5941 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6001 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6061 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6121 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6181 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6241 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6301 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6361 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6421 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6481 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6541 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6601 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6661 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6721 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6781 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6841 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6901 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6961 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7021 cctggccttt tgctggcctt ttgctcacat gt
//