pKSE-vCas9-ipB7RKD

LOCUS       pKSE-vCas9-ipB7RKD           18262 bp DNA     circular SYN 17-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pKSE-vCas9-ipB7RKD
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 18262)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..18262
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    127..551
                     /label=U6-26p
     misc_feature    830..1621
                     /label=SpR
     misc_feature    1773..1848
                     /label=gRNA scaffold
     misc_feature    1849..2040
                     /label=U6-26t
     misc_feature    2051..2941
                     /label=2x35Sp
     CDS             2998..3063
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3070..3090
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3115..7215
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDRGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVAEINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFRLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     variation       3274..3276
                     /label=S55R
     variation       6037..6039
                     /label=R976A
     variation       6118..6120
                     /label=K1003A
     variation       7051..7053
                     /label=T1314R
     RBS             7128..7136
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7216..7263
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    7270..7365
                     /label=32aa linker
     misc_feature    7366..9147
                     /label=piggyBac
     variation       8488..8490
                     /label=K375A
     CDS             9151..9171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9850..9866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9874..9890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9898..9928)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9943..9964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10155..10831
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             10894..11691
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    11748..11922
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12000..12024
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12449..13243
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13330..13918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    14104..14244
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      14592..14786
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14852..15925)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16354..16983)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc taagctcacg tgacggaatt
      121 aagcttcgac ttgccttccg cacaatacat catttcttct tagctttttt tcttcttctt
      181 cgttcataca gttttttttt gtttatcagc ttacattttc ttgaaccgta gctttcgttt
      241 tcttcttttt aactttccat tcggagtttt tgtatcttgt ttcatagttt gtcccaggat
      301 tagaatgatt aggcatcgaa ccttcaagaa tttgattgaa taaaacatct tcattcttaa
      361 gatatgaaga taatcttcaa aaggcccctg ggaatctgaa agaagagaag caggcccatt
      421 tatatgggaa agaacaatag tatttcttat ataggcccat ttaagttgaa aacaatcttc
      481 aaaagtccca catcgcttag ataagaaaac gaagctgagt ttatatacag ctagagtcga
      541 agtagtgatt gggagaccaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
      601 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      661 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
      721 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      781 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      841 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      901 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      961 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1021 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1081 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1141 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1201 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     1261 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     1321 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     1381 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     1441 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     1501 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1561 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1621 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     1681 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     1741 tatttttaag cgtgcataat aagccggtct cggttttaga gctagaaata gcaagttaaa
     1801 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttttgcaa
     1861 aattttccag atcgatttct tcttcctctg ttcttcggcg ttcaatttct ggggttttct
     1921 cttcgttttc tgtaactgaa acctaaaatt tgacctaaaa aaaatctcaa ataatatgat
     1981 tcagtggttt tgtacttttc agttagttga gttttgcagt tccgatgaga taaaccaata
     2041 agcttgcatg cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata
     2101 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
     2161 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
     2221 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
     2281 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     2341 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc
     2401 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
     2461 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
     2521 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
     2581 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
     2641 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     2701 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     2761 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg acctcaacac
     2821 aacatataca aaacaaacga atctcaagca atcaagcatt ctacttctat tgcagcaatt
     2881 taaatcattt cttttaaagc aaaagcaatt ttctgaaaat tttcaccatt tacgaacgat
     2941 actcgagggg gatccccaat acttgtatgg ccgcggccgc tctaggccta ctagatggat
     3001 tacaaggacc acgacgggga ttacaaggac cacgacattg attacaagga tgatgatgac
     3061 aagatggctc cgaagaagaa gaggaaggtt ggcatccacg gggtgccagc tgctgacaag
     3121 aagtactcga tcggcctcga tattgggact aactctgttg gctgggccgt gatcaccgac
     3181 gagtacaagg tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc
     3241 aagaagaatc tcattggcgc tctcctgttc gacagaggcg agacggctga ggctacgcgg
     3301 ctcaagcgca ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag
     3361 gagattttct ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag
     3421 tcattcctcg tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc
     3481 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg
     3541 gactccacag ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag
     3601 ttcaggggcc atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag
     3661 ctgttcatcc agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg
     3721 tcaggcgtcg acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gcgcctcgag
     3781 aacctgatcg cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg
     3841 ctcagcctgg ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag
     3901 ctgcagctct ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc
     3961 gatcagtacg cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct
     4021 gatattctca gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag
     4081 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc
     4141 cccgagaagt acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt
     4201 gacggcgggg cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg
     4261 gacggcacgg aggagctgct ggtgaagctc aatcgcgagg acctcctgag gaagcagcgg
     4321 acattcgata acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg
     4381 aggaggcagg aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt
     4441 ctgactttca ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct
     4501 tggatgaccc gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac
     4561 aagggcgcta gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca
     4621 aatgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag
     4681 ctgactaagg tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag
     4741 cagaagaagg ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag
     4801 ctcaaggagg actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt
     4861 gaggaccgct tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat
     4921 aaggacttcc tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc
     4981 actctgttcg aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc
     5041 gatgacaagg tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc
     5101 aggaagctca tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg
     5161 aagagcgatg gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca
     5221 ttcaaggagg atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat
     5281 atcgcgaacc tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt
     5341 gtggacgagc tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg
     5401 gcccgggaga atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg
     5461 atcgaggagg gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac
     5521 acgcagctgc agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat
     5581 gtggaccagg agctggatat taacaggctc agcgattacg acgtcgatca tatcgttcca
     5641 cagtcattcc tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac
     5701 cggggcaagt ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg
     5761 cgccagctcc tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct
     5821 gagaggggcg ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag
     5881 acacggcaga tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac
     5941 gatgagaatg acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc
     6001 tccgacttca ggaaggattt ccagttctac aaggttgcgg agatcaacaa ttaccaccat
     6061 gcccatgacg cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccagcg
     6121 ctcgagagcg agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc
     6181 aagtcggagc aggagattgg caaggctacc gccaagtact tcttctactc taacattatg
     6241 aatttcttca agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc
     6301 gagacgaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc
     6361 aggaaggttc tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc
     6421 gggttctcta aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag
     6481 aaggactggg atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc
     6541 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg
     6601 ctggggatca cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag
     6661 gcgaagggct acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc
     6721 ttcgagctgg agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg
     6781 aacgagctgg ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag
     6841 aagctcaagg gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag
     6901 cattacctcg acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc
     6961 gacgcgaatc tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg
     7021 gagcaggctg agaatatcat tcatctcttc cgcctgacga acctcggcgc ccctgctgct
     7081 ttcaagtact tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg
     7141 gacgcgaccc tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct
     7201 cagctcgggg gcgacaagcg gccagcggcg acgaagaagg cggggcaggc gaagaagaag
     7261 aagcttaaga gcggaggatc ttccggagga tctagcggct ccgagacacc aggaacatcc
     7321 gaaagcgcta caccagaatc tagcggaggc tcttccggag gatctatggg atccagtctc
     7381 gacgacgagc acatcttgtc cgcactactg caatccgacg acgaactggt cggggaagac
     7441 agtgattctg aagtaagcga ccatgtctcg gaggacgatg tgcagtcgga taccgaagag
     7501 gccttcattg acgaggttca tgaggttcag cccacgtctt caggatctga gattcttgat
     7561 gaacaaaatg ttattgagca gccgggctcg agcttggctt caaatagaat tttaacactg
     7621 cctcaaagga caatccgcgg caagaataag cactgctggt caacatctaa accaacgcgt
     7681 cgcagccgcg tgtctgcctt aaatatagtc aggtcccagc gcgggcctac aaggatgtgc
     7741 agaaacattt atgatcctct tctctgcttc aagctgttct tcaccgacga gataatctcc
     7801 gagatcgtca agtggactaa tgctgaaata tcgctcaagc gtagggagtc gatgacgtcc
     7861 gcaacattta gggatacgaa tgaggatgaa atttatgcat tttttgggat cctggttatg
     7921 actgctgtca ggaaggataa ccacatgtca actgatgacc tctttgatcg atcgttgtcc
     7981 atggtgtatg tttccgtcat gagtcgggac aggtttgatt tccttatacg gtgtctccgc
     8041 atggatgaca agagcatacg accaacatta agggaaaatg acgtcttcac gcccgttaga
     8101 aagatatggg acctcttcat tcatcagtgc attcagaact acacacccgg cgcccacttg
     8161 accattgatg agcaactttt gggttttcgc ggcagatgtc cgttcagagt gtacatcccc
     8221 aacaagccat ctaagtacgg tatcaaaata ctgatgatgt gtgactcggg aactaaatat
     8281 atgataaacg gcatgccata tcttgggcgg ggtactcaaa ccaatggtgt gccactaggc
     8341 gagtactatg tgaaagagct gtcgaagccg gtccacggaa gctgccgcaa catcacctgc
     8401 gacaactggt tcacttcgat ccctctcgcg aagaacttgc tacaggagcc ttacaagctc
     8461 acgatcgttg gaactgtggc cagcaacgca cgtgagatac cagaagtact gaaaaattca
     8521 cgaagtcggc ccgttggcac ttctatgttc tgctttgacg gcccgctcac tctcgtgtct
     8581 tataaaccga agcctgccaa gatggtttat cttttatcct cctgtgatga agatgcatcc
     8641 atcaatgaat ctacaggaaa gccacaaatg gtcatgtact acaaccaaac aaaagggggt
     8701 gtcgacactt tgaaccagat gtgcagcgtc atgacatgtt caaggaaaac caaccggtgg
     8761 ccgatggccc ttctctacgg catgattaac attgcctgta tcaactcctt catcatttac
     8821 tctcacaatg tgtcatcaaa gggagagaag gtacagagtc ggaagaagtt catgcgtaat
     8881 ctctacatgg gccttaccag tagcttcatg agaaagagac tggaggcgcc gaccctaaaa
     8941 aggtacctca gggataatat ctcaaacatc ttgcctaagg aagtaccagg aacgtcagat
     9001 gattccaccg aggagcctgt gatgaagaaa aggacctact gcacatattg cccgagcaaa
     9061 atacgcagaa aggcgtcggc ttcatgtaag aagtgcaaga aggtgatttg ccgggagcat
     9121 aatatcgaca tgtgccagtc ttgcttcgcg cccaaaaaaa aacgaaaagt gggcattcat
     9181 ggcgtgccgg cggcctgagc agagctttcg ttcgtatcat cggtttcgac aacgttcgtc
     9241 aagttcaatg catcagtttc attgcgcaca caccagaatc ctactgagtt tgagtattat
     9301 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     9361 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     9421 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     9481 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     9541 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta
     9601 tgcttattca ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga
     9661 atacaagtat gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca
     9721 gaatccttgt cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag
     9781 ttgagtatga aaatattttt taatgcattt tatgacttgc caattgattg acaacgaatt
     9841 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9901 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9961 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10021 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
    10081 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
    10141 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
    10201 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
    10261 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
    10321 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
    10381 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
    10441 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
    10501 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
    10561 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
    10621 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
    10681 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
    10741 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
    10801 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
    10861 tctctcgagc tttcgcagat ctgtcgatcg accatgggga ttgaacaaga tggattgcac
    10921 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
    10981 atcggctgct ctgatgccgc cgtgtttcgg ctgtcagcgc aggggcgccc ggttcttttt
    11041 gtcaagaccg acctgtccgg tgccctgaat gaactccagg acgaggcagc gcggctatcg
    11101 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
    11161 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
    11221 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
    11281 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
    11341 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
    11401 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacacat
    11461 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
    11521 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
    11581 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
    11641 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
    11701 tggggttcgg atcgatcctc tagctagagt cgatcgacaa gctcgagttt ctccataata
    11761 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
    11821 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
    11881 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg
    11941 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    12001 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    12061 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    12121 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    12181 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    12241 agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat
    12301 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
    12361 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
    12421 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
    12481 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
    12541 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
    12601 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
    12661 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
    12721 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
    12781 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
    12841 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
    12901 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
    12961 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
    13021 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
    13081 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
    13141 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
    13201 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac
    13261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    13321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    13381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    13441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    13501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    13561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    13621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    13681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    13741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    13801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    13861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    13921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    13981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    14041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    14101 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    14161 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    14221 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    14281 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    14341 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
    14401 gcgccggccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
    14461 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
    14521 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
    14581 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
    14641 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
    14701 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
    14761 ggttcccaat gtacgtgcta tccacaggaa acagaccttt tcgacctttt tcccctgcta
    14821 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
    14881 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
    14941 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
    15001 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
    15061 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
    15121 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
    15181 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
    15241 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
    15301 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
    15361 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
    15421 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
    15481 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
    15541 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    15601 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    15661 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    15721 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    15781 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    15841 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    15901 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    15961 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    16021 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    16081 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    16141 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    16201 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    16261 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    16321 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    16381 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    16441 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    16501 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    16561 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    16621 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    16681 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    16741 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    16801 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    16861 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    16921 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    16981 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    17041 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    17101 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    17161 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    17221 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    17281 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    17341 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    17401 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    17461 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    17521 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    17581 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    17641 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    17701 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    17761 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    17821 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    17881 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    17941 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    18001 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    18061 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    18121 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag
    18181 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc
    18241 ttttaaatat ccgttattct aa
//