| 质粒名称: | pcDNA3-myc-CUL3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 19893 |
| 存储实验室: | Yue Xiong |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3-myc |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5512bp |
| 完整质粒大小: | 8193bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | cullin 3、CUL3 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2304bp |
| GenBank编号 | NM_003590 |
| 标签/融合蛋白: | 3xmyc (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | KpnI (not destroyed) |
| 3’克隆位点: | XhoI (not destroyed) |
| 5’测序引物 | T7 |
| 3’测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3-myc-CUL3 was a gift from Yue Xiong (Addgene plasmid # 19893 ; http://n2t.net/addgene:19893 ; RRID:Addgene_19893) |
| 参考文献部分: | ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Mol Cell. 1999 Apr . 3(4):535-41. 10.1016/S1097-2765(00)80482-7 PubMed 10230407 |
买家导航
pcDNA3-myc-CUL3载体质粒基本信息
pcDNA3-myc-CUL3质粒图谱载体图谱和pcDNA3-myc-CUL3载体序列质粒序列多克隆位点信息
pcDNA3-myc-CUL3质粒载体简介
pcDNA3-myc-CUL3质粒序列载体序列
LOCUS pcDNA3-myc-CUL3 8175 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8175)
AUTHORS Ohta T, Michel JJ, Schottelius AJ, Xiong Y
TITLE ROC1, a homolog of APC11, represents a family of cullin partners
with an associated ubiquitin ligase activity.
JOURNAL Mol Cell. 1999 Apr . 3(4):535-41.
PUBMED 10230407
REFERENCE 2 (bases 1 to 8175)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.
1999 Apr . 3(4):535-41."
COMMENT Sequence Label: pcDNA3-myc-CUL3
FEATURES Location/Qualifiers
source 1..8175
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 663..692
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 699..728
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 735..764
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
promoter complement(3494..3512)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3495..3512)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3532..3549)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3538..3762
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3808..4236
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3895..3914)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4105..4126
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4245..4265)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4250..4579
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4430..4565
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4492..4511
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4646..5440
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4700..4719)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5310..5329
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5614..5735
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5651..5670)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5705..5724
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5784..5800)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5784..5800)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5797..5819)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5808..5824
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5832..5838,5839..5856,5857..5862))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5877..5898
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6015..6032)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6186..6774)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6266..6285)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6945..7736,7737..7805))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7568..7587
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7806..7910)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7985..8004)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagcta
661 tggagcaaaa gctcatctca gaggaagatc tggaattaga gcaaaagctc atctcagagg
721 aagatctgga attagagcaa aagctcatct cagaggaaga tctggaattc aggatcctga
781 tatccgcggt accgagcacc atgtcgaatc tgagcaaagg cacgggcagc cggaaggaca
841 ccaagatgcg gatccgggcc tttccgatga ccatggatga aaaatatgta aacagcattt
901 gggaccttct gaaaaatgca attcaagaaa tccagcgtaa gaataacagt ggtcttagtt
961 ttgaggagct ctatagaaat gcatatacaa tggttttgca taaacatgga gaaaagctct
1021 acactggact aagagaagtt gttaccgaac atctcataaa taaggtgcga gaagatgtac
1081 taaattcatt gaataacaac tttcttcaaa cgctaaatca agcttggaat gatcatcaaa
1141 cagctatggt gatgattaga gacatactaa tgtacatgga ccgtgtgtat gtacaacaaa
1201 ataatgtgga gaacgtctac aatttgggat taattatttt tcgagatcaa gttgtacgtt
1261 atgggtgtat tagggatcat ctacggcaaa ctctattgga tatgattgca agagagcgga
1321 aaggagaagt cgtagacaga ggcgcaataa gaaatgcttg ccagatgtta atgattttag
1381 gtctcgaagg aagatcagtc tgtgaagaag attttgaggc tccttttttg gaaatgtctg
1441 cagaattttt tcagatggaa agccagaaat ttttagcaga aaatagtgct tcagtatata
1501 taaagaaagt agaagctaga attaatgaag aaatagaacg agtgatgcac tgccttgaca
1561 aatcaacgga agaaccaatt gtaaaggtgg ttgaaaggga actcatttcc aagcacatga
1621 agactatagt agaaatggag aattctgggc tagtacatat gttgaaaaat ggaaagacag
1681 aagaccttgg ttgcatgtac aagttattta gtcgtgtgcc aaatggtttg aaaacaatgt
1741 gtgagtgtat gagttcctat ttgagggagc aaggtaaagc tcttgtttct gaagaaggag
1801 aaggaaagaa tcctgttgac tatatccagg gcttattgga tctgaagagt aggttcgatc
1861 gcttcctcct ggaatcattc aacaatgacc gtctctttaa acaaactatt gcgggtgact
1921 ttgagtattt tctcaacctc aactccaggt ctcctgaata cctctcatta tttattgatg
1981 ataagctgaa aaagggagtc aaagggctaa cagaacaaga agtagaaaca atattggata
2041 aagcaatggt cctttttagg tttatgcaag aaaaagatgt atttgaacgt tattataaac
2101 aacacttggc aaggagactt ctcacaaata aaagtgtttc tgatgactct gaaaaaaaca
2161 tgatatctaa gttaaagact gaatgtggat gtcagttcac gtcaaaactg gaaggaatgt
2221 ttagggatat gagcatctca aacacaacga tggatgaatt caggcaacat ctacaggcaa
2281 ctggtgtatc tttaggtggt gttgatctta cagtccgggt gctcacgaca ggatattggc
2341 ccactcagtc agccacacca aagtgcaaca tcccaccagc accaagacat gcttttgaga
2401 tattcagaag gttctactta gccaaacaca gtggtcgaca gctcacactc cagcatcata
2461 tgggttctgc agatctcaat gccacatttt atggaccagt taaaaaggaa gatggatctg
2521 aagttggtgt tggaggtgca caagtaactg gctctaatac acggaagcac atattgcaag
2581 tttccacttt ccagatgacc atattaatgc tctttaataa tagagaaaaa tacacatttg
2641 aggaaattca gcaagagaca gatatccctg aaagagagct tgttagagcc ctacagtccc
2701 tcgcctgtgg taaaccaaca cagcgggttc ttacaaaaga acccaaatca aaggaaatag
2761 aaaatggtca tatatttaca gttaatgatc aattcacatc caaactacac agagtcaaga
2821 ttcaaacagt tgctgccaaa caaggtgaat ccgacccaga gaggaaagaa acaaggcaga
2881 aagtagacga cgacagaaaa catgagatag aagctgctat agtgcggata atgaaatcta
2941 gaaagaagat gcagcacaat gttctagtag cggaggtaac tcagcagttg aaggcgcgat
3001 tcttaccaag tccagttgtt attaagaaac gtattgaagg acttattgag agagaatatt
3061 tggcacgaac acctgaggat cgcaaagtat acacatatgt agcataaaat gcgttcagaa
3121 atttgattta ttcttggact gtactcttcg catggactgg gaagttcttt taaatcatta
3181 aatattaaga cgaccatctc ttctattaaa ttacagtaca tgttctagac cattgagatc
3241 aagcctttac tccctttgag agtttccaac atcagttgat tgagcttcag gctttacaac
3301 gtttatccct gtagagatca tctttacagt tcctcgggaa aatgtgaatg tgctgcgttt
3361 tgttttcttt actgtatgaa aacaggaaaa taaaagagaa atttagaaaa tacagctcat
3421 tacaataaaa ttgttggatt tcatttcaaa aaaaaaaaaa aaaaaactcg agcatgcatc
3481 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
3541 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
3601 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
3661 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
3721 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
3781 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
3841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
3901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
3961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4021 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
4141 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
4201 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
4261 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
4321 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
4381 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
4441 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
4501 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
4561 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
4621 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
4681 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
4741 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
4801 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
4861 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
4921 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
4981 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
5041 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
5101 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
5161 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
5221 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
5281 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
5341 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
5401 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
5461 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
5521 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
5581 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
5641 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
5701 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
5761 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
5821 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
5881 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
5941 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
6001 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
6061 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
6121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
6181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
6241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
6301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
6361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
6421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
6481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
6541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
6601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
6661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
6721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
6781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
6841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
6901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
6961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
7021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
7081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
7141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
7201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
7261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
7321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
7381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
7441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
7501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
7561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
7621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
7681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
7741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
7801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
7861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
7921 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
7981 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
8041 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
8101 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
8161 ccagatatac gcgtt
//
