| 质粒名称: | GST-HA-GFP11-N1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 91953 |
| 存储实验室: | Rong Li |
|
载体骨架基本信息 |
|
| 载体名称: | pAcGFP1-N1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Kan/Neo |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GST-HA-GFP11 |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII（销毁后未知） |
| 3' 克隆位点: | NotI（销毁后未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | GST-HA-GFP11-N1 was a gift from Rong Li (Addgene plasmid # 91953 ; http://n2t.net/addgene:91953 ; RRID:Addgene_91953) |
| 参考文献部分: | Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R. Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695. Epub 2017 Mar 1. 10.1038/nature21695 PubMed 28241148 |
买家导航
GST-HA-GFP11-N1载体质粒基本信息
GST-HA-GFP11-N1质粒图谱载体图谱和GST-HA-GFP11-N1载体序列质粒序列多克隆位点信息
GST-HA-GFP11-N1质粒载体简介
GST-HA-GFP11-N1质粒序列载体序列
LOCUS GST-HA-GFP11-N1 4759 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses GST-HA-GFP11 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS GST-HA-GFP11-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4759)
AUTHORS Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z, Florens L, Li R
TITLE Cytosolic proteostasis through importing of misfolded proteins into
mitochondria.
JOURNAL Nature. 2017 Mar 16;543(7645):443-446. doi: 10.1038/nature21695.
Epub 2017 Mar 1.
PUBMED 28241148
REFERENCE 2 (bases 1 to 4759)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4759
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 124..427
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 428..631
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 582..602
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 691..1344
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
primer_bind 1302..1324
/label=pGEX 5'
/note="pGEX vectors, Glutathione-S-transferase, forward
primer"
CDS 1372..1398
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1438..1485
/codon_start=1
/product="11th beta-strand of superfolder GFP"
/label=GFP11
/note="associates with GFP(1-10), consisting of the first
10 beta-strands, to reconstitute fluorescent GFP (Cabantous
et al., 2005; Kamiyama et al., 2016)"
/translation="RDHMVLHEYVNAAGIT"
polyA_signal 1610..1731
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1647..1666)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1701..1720
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1738..2193)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1875..1896)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2087..2106
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2220..2324
/gene="bla"
/label=AmpR promoter
promoter 2326..2683
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2349..2369)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2534..2669
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2596..2615
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2718..3512
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2772..2791)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3382..3401
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3700..3719)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3744..3791
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4120..4708
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4609..4628
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
61 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
121 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
181 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
241 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
301 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
361 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
421 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
481 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
541 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
601 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
661 ctaccggact cagatctcga gctcaagctt atgtccccta tactaggtta ttggaaaatt
721 aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag
781 catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg
841 gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc
901 atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca
961 gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca
1021 tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg
1081 aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat
1141 cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg
1201 gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat
1261 aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt
1321 ggtggtggcg accatcctcc aaaaggatcc ggtgacggtc cggtcgccac ctacccatac
1381 gatgttccag attacgctgg tcgacggatc ggtggtggtt ctggaggcgg ttctatgaga
1441 gatcatatgg ttttgcatga atatgttaat gctgctggta ttacttaagc ggccgcgact
1501 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
1561 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
1621 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
1681 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
1741 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
1801 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
1861 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
1921 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
1981 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
2041 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
2101 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
2161 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
2221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
2281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
2341 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
2401 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
2461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
2521 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
2581 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
2641 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
2701 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
2761 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
2821 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
2881 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
2941 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3001 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3061 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3121 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3181 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3241 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
3301 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3361 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3421 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3481 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3541 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3601 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3661 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
3721 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
3781 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
3841 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
3901 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
3961 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
4021 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
4081 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
4141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
4201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
4261 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
4321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
4381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
4441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
4501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
4561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
4621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
4681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
4741 ggccttttgc tggcctttt
//
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