pQL166

LOCUS       pQL166              5560 bp DNA     circular SYN 08-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5560
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     174..227
                     /label=QL229 99-F
     primer_bind     247..269
                     /label=Oscl23-1
     primer_bind     251..272
                     /label=引物 1
     misc_feature    253..400
                     /label=hcmvmin
     misc_feature    322..336
                     /label=New Feature
     primer_bind     complement(391..411)
                     /label=ql228 98-R
     primer_bind     392..431
                     /label=QL 431-F
     CDS             412
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation=""
     CDS             415..1119
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1099..1131)
                     /label=QL138-R
     primer_bind     complement(1102..1122)
                     /label=QL144-R
     primer_bind     complement(1102..1122)
                     /label=引物 2
     primer_bind     complement(1103..1142)
                     /label=QL 430-R
     terminator      1157..1384
                     /label=bGH PA
     misc_feature    2052..2129
                     /label=SV40 ORI
     CDS             2271..3062
                     /label=Kan/neoR
     primer_bind     complement(2766..2775)
                     /label=oYW232
                     /note="fLuc-qPCR-F"
     primer_bind     complement(3402..3422)
                     /label=M13-rev
     misc_binding    complement(3428..3450)
                     /label=LacO
     promoter        complement(3455..3484)
                     /label=lac
     CDS             complement(4567..5226)
                     /label=AmpR
     promoter        complement(5466..5494)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttttcgcg
      241 cggtttgcta gctcgagctc ggtacccggg tcgagtaggc gtgtacggtg ggaggcctat
      301 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      361 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt catggtgagc
      421 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      481 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      541 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      601 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      661 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      721 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      781 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
      841 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
      901 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
      961 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     1021 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     1081 ggccgccact ccaccggcgg catggacgag ctgtacaagt aatctagagg gcccgtttaa
     1141 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     1201 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     1261 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     1321 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     1381 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     1441 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1501 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1561 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     1621 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     1681 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     1741 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     1801 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     1861 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1921 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     1981 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2041 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2101 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     2161 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     2221 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     2281 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     2341 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     2401 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     2461 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     2521 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     2581 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     2641 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     2701 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     2761 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     2821 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     2881 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     2941 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     3001 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     3061 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     3121 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     3181 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     3241 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3301 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3361 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     3421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3481 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3541 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3601 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4321 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4561 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4621 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4681 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4741 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4801 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4861 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4921 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4981 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5041 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5101 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5161 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5221 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5281 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5341 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5401 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5461 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5521 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//