| 出品公司: | ATCC |
|---|---|
| 载体名称: | pRS304 |
| 质粒类型: | 酵母表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T3 |
| 载体大小: | 4.3 kb |
| 5’测序引物: | M13-F：TGTAAAACGACGGCCAGT |
| 3’测序引物: | M13-R：CAGGAAACAGCTATGACC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 亮氨酸筛选 |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 酿酒酵母 |
| 产品目录号: | 77139 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 备注: | pRS304载体是酿酒酵母表达载体。 |
买家导航
pRS304载体质粒基本信息
pRS304质粒图谱载体图谱和pRS304载体序列质粒序列多克隆位点信息
pRS304质粒载体简介
pRS304质粒序列载体序列
LOCUS pRS304 4267 bp DNA circular SYN 07-MAR-2024
DEFINITION Yeast integrative vector with a TRP1 marker and an MCS derived from
pBLUESCRIPT.
ACCESSION U03436
VERSION .
KEYWORDS pRS304
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4267)
AUTHORS Sikorski RS, Hieter P.
TITLE A system of shuttle vectors and yeast host strains designed for
efficient manipulation of DNA in Saccharomyces cerevisiae.
JOURNAL Genetics 1989;122:19-27.
PUBMED 2659436
REFERENCE 2 (bases 1 to 4267)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 14, 2024 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics";
date: "1989"; volume: "122"; pages: "19-27"
FEATURES Location/Qualifiers
source 1..4267
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 185..465
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
/note="color: #ffffff; direction: RIGHT"
CDS 466..1140
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/note="color: #ff7f50"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
rep_origin complement(1237..1692)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
/note="color: #ffff00"
CDS complement(1475..2050)
/codon_start=1
/gene="lacZ fragment"
/product="fragment of beta-galactosidase"
/label=lacZ'
/note="color: #993366"
/translation="MTMITPSSELTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPLV
LERPPPRWSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG
FPRQALNRGLPLGFRFSALRHLDPKKLD"
primer_bind 1837..1853
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
promoter 1860..1878
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: RIGHT"
misc_feature 1887..1994
/label=MCS
/note="pBluescript multiple cloning site"
/note="color: #99ccff"
promoter complement(2007..2025)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
primer_bind complement(2046..2062)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
protein_bind 2070..2086
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
promoter complement(2094..2124)
/label=lac promoter
/note="promoter for the E. coli lac operon"
/note="This reverse directional feature has 3 segments:
1: 2094 .. 2100 / #ffffff / -10
2: 2101 .. 2118 / #ffffff
3: 2119 .. 2124 / #ffffff / -35"
rep_origin complement(2448..3036)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(3207..4067)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 3207 .. 3998 / #ccffcc
2: 3999 .. 4067 / #ccffcc / signal sequence
Cleavage site after base 3998"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4068..4172)
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: LEFT"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accaaacgac attactatat atataatata ggaagcattt aatagacagc atcgtaatat
241 atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa
301 atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg ccgattaaga
361 attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
421 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat
481 ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca
541 gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag
601 agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat
661 agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt
721 ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg
781 caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac
841 tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat
901 tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga
961 aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac gccagaaaat
1021 gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag
1081 acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag
1141 gttattactg agtagtattt atttaagtat tgtttgtgca cttgcctgcg gtgtgaaata
1201 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt
1261 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
1321 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
1381 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
1441 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
1501 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
1561 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
1621 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
1681 tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
1741 tgcgggcctc ttcgctatta cgccagctgg cgaagggggg atgtgctgca aggcgattaa
1801 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
1861 aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaacta
1921 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
1981 gggggcccgg tacccagctt ttgttccctt tagtgagggt taattccgag cttggcgtaa
2041 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
2101 ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta
2161 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
2221 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
2281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
2341 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
2401 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
2461 ggcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
2521 ggactataaa gataccaggc gttcccccct ggaagctccc tcgtgcgctc tcctgttccg
2581 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
2641 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
2701 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
2761 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
2821 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
2881 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
2941 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
3001 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
3061 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
3121 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
3181 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
3241 gcgatctgtc tatttcgttc atccatagtt gcctgactgc ccgtcgtgta gataactacg
3301 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
3361 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
3421 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
3481 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
3541 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
3601 tgatccccca tgttgtgaaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
3661 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
3721 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
3781 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
3841 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
3901 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
3961 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
4021 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
4081 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
4141 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
4201 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
4261 tttcgtc
//
