| 质粒名称: | p52sheLL |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 46950 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 博来霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV52L1+L2,p52LLw |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p52sheLL was a gift from John Schiller (Addgene plasmid # 46950 ; http://n2t.net/addgene:46950 ; RRID:Addgene_46950) |
| 参考文献部分: | Modification of human papillomavirus-like particle vaccine by insertion of the cross-reactive L2-epitopes. Kondo K, Ochi H, Matsumoto T, Yoshikawa H, Kanda T. J Med Virol. 2008 May;80(5):841-6. doi: 10.1002/jmv.21124. 10.1002/jmv.21124 PubMed 18360909 |
买家导航
p52sheLL载体质粒基本信息
p52sheLL质粒图谱载体图谱和p52sheLL载体序列质粒序列多克隆位点信息
p52sheLL质粒载体简介
p52sheLL质粒序列载体序列
LOCUS p52sheLL 10725 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION HPV52 L1 and L2 Bicistronic, too large to self-package.
ACCESSION .
VERSION .
KEYWORDS p52sheLL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10725)
AUTHORS Kondo K, Ochi H, Matsumoto T, Yoshikawa H, Kanda T
TITLE Modification of human papillomavirus-like particle vaccine by
insertion of the cross-reactive L2-epitopes.
JOURNAL J Med Virol. 2008 May;80(5):841-6. doi: 10.1002/jmv.21124.
PUBMED 18360909
REFERENCE 2 (bases 1 to 10725)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10725
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3662..4250
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3715..3735)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4133..4144)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4287..4304)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4293..4517
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4563..4991
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4650..4669)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4860..4881
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5000..5020)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 6949..7317
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 7447..7568
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7484..7503)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7538..7557
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7617..7633)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7617..7633)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7630..7652)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7641..7657
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7665..7695)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7710..7731
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7848..7865)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8019..8604)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8099..8118)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8775..9635)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9398..9417
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9636..9740)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9815..9834)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 10006..10385
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 10386..10589
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 10540..10560
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 10591..10609
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10612..10630
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 10640..10664
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 tccggagggc cgcgccacca tgagcgtgtg gaggcccagc gaggccaccg tgtacctgcc
61 ccccgtgccc gtgagcaagg tggtgagcac cgacgagtac gtgagcagga ccagcatcta
121 ctactacgcc ggcagcagca ggctgctgac cgtgggccac ccctacttca gcatcaagaa
181 caccagcagc ggcaacggca agaaggtgct ggtgcccaag gtgagcggcc tgcagtacag
241 ggtgttcagg atcaagctgc ccgaccccaa caagttcggc ttccccgaca ccagcttcta
301 caaccccgag acccagaggc tggtgtgggc ctgcaccggc ctggagatcg gcaggggcca
361 gcccctgggc gtgggcatca gcggccaccc cctgctgaac aagttcgacg acaccgagac
421 cagcaacaag tacgccggca agcccggcat cgacaacagg gagtgcctga gcatggacta
481 caagcagacc cagctgtgca tcctgggctg caagcccccc atcggcgagc actggggcaa
541 gggcaccccc tgcaacaaca acagcggcaa ccccggcgac tgcccccccc tgcagctgat
601 caacagcgtg atccaggacg gcgacatggt ggacaccggc ttcggctgca tggacttcaa
661 caccctgcag gccagcaaga gcgacgtgcc catcgacatc tgcagcagcg tgtgcaagta
721 ccccgactac ctgcagatgg ccagcgagcc ctacggcgac agcctgttct tcttcctgag
781 gagggagcag atgttcgtga ggcacttctt caacagggcc ggcaccctgg gcgaccccgt
841 gcccggcgac ctgtacatcc agggcagcaa cagcggcaac accgccaccg tgcagagcag
901 cgccttcttc cccaccccca gcggcagcat ggtgaccagc gagagccagc tgttcaacaa
961 gccctactgg ctgcagaggg cccagggcca caacaacggc atctgctggg gcaaccagct
1021 gttcgtgacc gtggtggaca ccaccaggag caccaacatg accctgtgcg ccgaggtgaa
1081 gaaggagagc acctacaaga acgagaactt caaggagtac ctgaggcacg gcgaggagtt
1141 cgacctgcag ttcatcttcc agctgtgcaa gatcaccctg accgccgacg tgatgaccta
1201 catccacaag atggacgcca ccatcctgga ggactggcag ttcggcctga cccccccccc
1261 cagcgccagc ctggaggaca cctacaggtt cgtgaccagc accgccatca cctgccagaa
1321 gaacaccccc cccaagggca aggaggaccc cctgaaggac tacatgttct gggaggtgga
1381 cctgaaggag aagttcagcg ccgacctgga ccagttcccc ctgggcagga agttcctgct
1441 gcaggccggc ctgcaggcca ggcccaagct gaagaggccc gccagcagcg cccccaggac
1501 cagcaccaag aagaagaagg tgaagaggtg aaagcttttt gaattctttg gatccactag
1561 ggctcgagga agcttaaaac agctctgggg ttgtacccac cccagaggcc cacgtggcgg
1621 ctagtactcc ggtattgcgg tacccttgta cgcctgtttt atactccctt cccgtaactt
1681 agacgcacaa aaccaagttc aatagaaggg ggtacaaacc agtaccacca cgaacaagca
1741 cttctgtttc cccggtgatg tcgtatagac tgcttgcgtg gttgaaagcg acggatccgt
1801 tatccgctta tgtacttcga gaagcccagt accacctcgg aatcttcgat gcgttgcgct
1861 cagcactcaa ccccagagtg tagcttaggc tgatgagtct ggacatccct caccggtgac
1921 ggtggtccag gctgcgttgg cggcctacct atggctaacg ccatgggacg ctagttgtga
1981 acaaggtgtg aagagcctat tgagctacat aagaatcctc cggcccctga atgcggctaa
2041 tcccaacctc ggagcaggtg gtcacaaacc agtgattggc ctgtcgtaac gcgcaagtcc
2101 gtggcggaac cgactacttt gggtgtccgt gtttcctttt attttattgt ggctgcttat
2161 ggtgacaatc acagattgtt atcataaagc gaattggatt gcggccgcgc caccatgagg
2221 tacaggagga gcaccaggca caagagggcc agcgccaccc agctgtacca gacctgcaag
2281 gccagcggca cctgcccccc cgacgtgatc cccaaggtgg agggcaccac catcgccgac
2341 cagctgctga agtacggcag cctgggcgtg ttcttcggcg gcctgggcat cggcaccggc
2401 gccggcagcg gcggcagggc cggctacgtg cccctgagca ccaggccccc caccagcagc
2461 atcaccacca gcaccatcag gccccccgtg accgtggagc ccatcggccc cctggagccc
2521 agcatcgtga gcatgatcga ggagaccacc ttcatcgaga gcggcgcccc cgcccccagc
2581 atccccagcg ccaccggctt cgacgtgacc accagcgcca acaacacccc cgccatcatc
2641 aacgtgacca gcatcggcga gagcagcgtg cagagcgtga gcacccacct gaaccccacc
2701 ttcaccgagc ccagcatcat ccagcccccc gcccccgccg aggccagcgg ccacgtgctg
2761 ttcagcagcc ccaccatcag cacccacacc tacgaggaga tccccatgga caccttcgtg
2821 accagcaccg acagcagcag cgtgaccagc agcaccccca tccccggcag caggcccacc
2881 accaggctgg gcctgtacag cagggccacc cagcaggtga aggtggtgga ccccgccttc
2941 atgagcagcc cccagaagct ggtgacctac aacaaccccg tgttcgaggg cgtggacacc
3001 gacgagacca tcatcttcga caggagccag ctgctgcccg cccccgaccc cgacttcctg
3061 gacatcatcg ccctgcacag gcccgccctg accagcagga ggggcaccgt gaggttcagc
3121 aggctgggca acaaggccac cctgaggacc aggagcggca agcagatcgg cgccagggtg
3181 cactactacc acgacatcag ccccatccag cccgccgagg tgcaggagga catcgagctg
3241 cagcccctgc tgccccagag cgtgagcccc tacaccatca acgacggcct gtacgacgtg
3301 tacgccgaca gcctgcagca gcccaccttc cacctgccca gcaccctgag cacccacaac
3361 aacaccttca ccgtgcccat caacagcggc atcgacttcg tgtaccagcc caccatgagc
3421 atcgagagcg gccccgacat ccccctgccc agcctgccca cccacacccc cttcgtgccc
3481 atcgccccca ccgcccccag caccagcatc atcgtggacg gcaccgactt catcctgcac
3541 cccagctact tcctgctgag gaggaggagg aagaggttcc cctacttctt caccgacgtg
3601 agggtggccg cctgaaagct ttgaattctt tggatccact agcgatatca agcttatcga
3661 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
3721 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
3781 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
3841 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
3901 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
3961 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
4021 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
4081 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
4141 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
4201 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
4261 tcggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
4321 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
4381 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
4441 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
4501 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
4561 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
4621 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
4681 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
4741 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
4801 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
4861 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
4921 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
4981 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
5041 ccagcaggca gaagtatgca aagcatgcag aattctatca aatatttaaa gaaaaaaaaa
5101 ttgtatcaac tttctacaat ctctttcaga agacagaagc agagggaata cttcctaaat
5161 cattcaacta ggccagcatt accttaatac cggaactaga aaatgacatt acaagaaaag
5221 aaaacaacag accaatatct ctcatgaaca aagatacaaa cattttcaac aaaatattag
5281 caaaaagaat ccaagaatgt atcaaaaaat atacaccaca accaagtaga atttattcca
5341 gatatgtaag ggtggttcaa cgtttgaaaa tcaattaacg taatttgtcc catcaacagg
5401 ttaaagaaga aaatcacatg gtcatattga tagacacaga aaaagcattt gacaaaattt
5461 aacacccatt catgatgcaa tctctcagta aactaggaat agaggaaaac ttcctcagct
5521 tgaatgtacc ttcctctcaa ttttgctatg aacctgaaac tcctcttaaa aaataaagtt
5581 tttcatttaa aaagaaaaca aaaaacatgg aggagcgttg atgtatctca ttttagacca
5641 atcagctatg gatagttagg cgacagcaca gatagctgct gtacttctgt ttctggcaat
5701 gttccagact acatttaaaa aatttttaat tatagacttg tacttaatgt tcaagaaaaa
5761 tatgaaaatg gctttgccgt gttaatgcta ctctttttta aaaaaaacta aagttcaaac
5821 tttatttata tttcattagt tttttagcta ctgttctttt tctgttctgg gatctcattc
5881 agaatgccac attacatata attctcatgt ctccttgggt tcctcttagt tttgacagtt
5941 cctcagactt ttcttatttt tgatgacctt gacagttttg aggagtactg gttagatata
6001 gggtaatggt ttttaaagta tatttgtcat gatttatact ggggtaaggg tttggggagg
6061 aagcccatgg ggtaaagtac tgttctcatc acatcatatc aaggttatat accatcaata
6121 ttgccacaga tgttacttag ccttttaata tttctctaat ttagtgtata tgcaatgata
6181 gttctctgat ttctgagatt gagtttctca tgtgtaatga ttatttagag tttctctttc
6241 atctgttcaa atttttgtct agttttattt tttactgatt tgtaagactt ctttttataa
6301 tctgcatatt acaattctct ttactggggt gttgcaaata ttttctgtca ttctatggcc
6361 tgacttttct taatggtttt ttaattttaa aaataagtct taatattcat gcaatctaat
6421 taacaatctt ttctttgtgg ttaggacttt gagtcataag aaatttttct ctacactgaa
6481 gtcatgatgg catgcttcta tattattttc taaaagattt aaagttttgc cttctccatt
6541 tagacttata attcactgga atttttttgt gtgtatggta tgacatatgg gttccctttt
6601 attttttaca tataaatata tttccctgtt tttctaaaaa agaaaaagat catcattttc
6661 ccattgtaaa atgccatatt tttttcatag gtcacttaca tatatcaatg ggtctgtttc
6721 tgagctctac tctattttat cagcctcact gtctatcccc acacatctca tgctttgctc
6781 taaatcttga tatttagtgg aacattcttt cccattttgt tctacaagaa tatttttgtt
6841 attgtctttt gggcttctat atacatttta gaatgaggtt ggcaagttaa caaacagctt
6901 ttttggggtg aacatattga ctacaaattt atgtggaaag aaagtaccaa gttgaccagt
6961 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
7021 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
7081 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
7141 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
7201 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
7261 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
7321 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
7381 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
7441 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
7501 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
7561 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
7621 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
7681 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
7741 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
7801 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
7861 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
7921 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
7981 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
8041 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
8101 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
8161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
8221 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
8281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
8341 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
8401 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
8461 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
8521 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt
8581 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
8641 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
8701 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
8761 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
8821 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
8881 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
8941 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
9001 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
9061 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
9121 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
9181 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
9241 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
9301 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
9361 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
9421 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
9481 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
9541 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
9601 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
9661 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
9721 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
9781 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
9841 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
9901 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
9961 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
10021 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
10081 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
10141 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
10201 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
10261 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
10321 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
10381 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
10441 gatttccaag tctccacccc attgacgtca atgggagttt gttttggaac caaaatcaac
10501 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
10561 tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat ctccctatca
10621 gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
10681 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcc
//
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